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Citations for abcam :
1901 - 1950 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Microbiology 2022Quote: The obtained proteins were separated by 12% SDS–PAGE gel and immunoblotted using anti-GFP (1:5000 dilution, Abcam, ab183734), anti-RFP (1:5000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-G3BP1 beads were generated by incubating 200 μL Protein-G beads with 20 μL anti-G3BP1 antibody (Abcam, ab290) in 1 mL Buffer B (200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Microbiology 2023Quote: ... the cells were then incubated with the following primary antibodies in 0.1% BSA solution (1X PBS) overnight in the dark at 4 °C: mouse monoclonal (1:1000) Respiratory Syncytial Virus (F-protein) (Abcam), rabbit monoclonal (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with the polyclonal rabbit anti- myelin basic protein (MBP) antibody (1:200, ab40390, Abcam) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to PVDF membranes and then immunoblotted with the following antibodies: cleaved Caspase-3 (1:500, Abcam, ab13847), WAVE1 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Molecular Biology 2022Quote: ... The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211) because detection via our anti-DnaD antibody (Eurogentec ...
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bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...
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bioRxiv - Pathology 2023Quote: ... Five micrograms of extracted protein was used for western blot analysis with primary antibody against MMP2 (1:200; ab92536, Abcam). The signal intensity of bands was quantified using the Image J densitometry software (NIH).
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Systems Biology 2023Quote: ... The protein expression and solubility were evaluated by both Coomassie stain and western blot (WB) of the GST and MBP fusion proteins using anti-GST (abcam) and anti-MBP (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: Concentrations of plasma LPS-binding protein (LBP) were examined via Mouse LBP SimpleStep ELISA according to the manufacturer’s protocol (Abcam, #ab269542). Plasma samples were prepared at a 1:16,000 dilution ...
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bioRxiv - Genetics 2023Quote: ... and Atg18 knockdown flies with the expression of mCherry-Atg8a fusion protein were counterstained with diluted CytoPainter Phalloidin-iFlor 488 (from Abcam) for F- actin at room temperature for 15 minutes before the tissues were mounted in phosphate-buffered saline solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Cell Biology 2023Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Genetics 2024Quote: ... Protein G beads (80 uL) and lysates were washed with RIPA buffer and immunoprecipitated with antibodies targeting H3K18ac (Abcam #ab40888), H3K18pr (PTM Bio #PTM-213) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stained for 10 minutes at 37°C with a chicken anti-green fluorescent protein (GFP) antibody (cross-reactive with YFP; ab13970, AbCam) diluted 1:1000 in neurobasal medium to stain for surface GluN2B ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and size of the protein has been evaluated by Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Synthetic Biology 2024Quote: ... Percent purity of each sample was determined by running diluted aliquots of each purified protein on a 4-12% Bis-Tris gel and staining with Optiblot Blue (Abcam). After destaining ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-voltage-dependent anion-selective channel protein 1 (VDAC1)/porin (mouse monoclonal, #ab14734, RRID: AB_443084, Abcam; 1:2000 for WB), anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The co-immunoprecipitated and immunoprecipitated proteins were then characterized by Western Blotting using anti-HA monoclonal antibody (ab18181, Abcam, USA) and anti-myc monoclonal antibody (ab18185 ...
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bioRxiv - Microbiology 2024Quote: Lysates were incubated over night at 4°C with 40 µL of Dynabeads protein G beads conjugated with 2 µg rabbit anti-DDX5 antibody (ab21696; Abcam), or rabbit anti-DDX17 antibody (19910-1-AP ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... NAD and NADH extractions were performed on 20mg PBS washed fresh tissue on wet ice homogenised with a Dounce homogeniser with 400uL of NADH/NAD extraction buffer (Abcam NAD/NADH Assay kit-ab65348). For details ...
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bioRxiv - Cell Biology 2022Quote: ... the Apoptosis and DNA damage (H2A.X(S139) + cleaved PARP1 + Anti-GAPDH) Western Blot Cocktail (ab131385) was obtained from Abcam. Mouse and Rabbit polyclonal secondary antibodies were purchased from Abcam (ab97023 and ab97051 respectively).
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bioRxiv - Cell Biology 2020Quote: ... Between 300 and 500 µg of proteins were incubated with 1 µg of antibody (when using Santa Cruz or Abcam antibodies) or with the concentration recommended by the manufacturer (when using Cell Signaling antibodies ...
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bioRxiv - Biochemistry 2021Quote: ... Resin was washed 4X in 800 uL EMB 300 Buffer and then protein association was analyzed by Western blot using antibody for hnRNP A2/B1 (Abcam #ab6102). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Our antibody against DARPP-32 binds to a 16 AA segment on a region of the protein that does not contain any phosphorylation sites (Abcam ab40801) and therefore it labels DARPP-32 irrespective of phosphorylation state and cellular location ...
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bioRxiv - Immunology 2020Quote: ... For selected experiments T cells were cultured in serum-depleted medium with or without native human C1q protein (100ug/ml, Abcam, ab96363).
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bioRxiv - Cancer Biology 2019Quote: ... and incubated overnight at 4 °C with Protein G magnetic beads (Dynabeads: ThemoFisher) that had been pre-incubated with anti-H3K27Ac (Abcam, ab4729) or anti-Sox2 (R&D ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were blocked with 5% fish gelatine before incubation with the primary antibodies for myelin basic protein (MBP, Abcam, 1:500) and protein gene product 9.5 (PGP ...
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bioRxiv - Systems Biology 2020Quote: ... The blot was probed with the antibody against the viral nucleocapsid protein (Cambridgebio, USA) and the horseradish peroxidase-conjugated Goat Anti-Rabbit IgG (Abcam, USA) as the primary and the secondary antibodies ...
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bioRxiv - Genetics 2020Quote: ... Reactions were stopped by addition of 6x SDS Laemmli buffer and proteins were resolved by 15% SDS-PAGE and analysed by western blotting with anti-ubiquitin antibodies (1:2000 Abcam P4D1) and anti-Rad51 (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... Staining was completed per manufacturer’s instructions for RNAScope® multiplex V2 detection kit (ACD Bio) coupled with immunofluorescent protein staining for either Cytokeratin19 (ab52625 Abcam) at 1:250 dilution ...
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bioRxiv - Bioengineering 2020Quote: Quantification of proteins via C-terminal GFP tags was performed using a GFP quantification kit from AbCam (Cambridge, UK, product # ab171581) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The monoclonal anti-VGLUT2 antibody was raised in Mice immunized to a full length recombinant protein corresponding to rat VGLUT2 (Abcam, ab79157) and has been previously shown to stain VGLUT2 in zebra finches (86) ...
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bioRxiv - Neuroscience 2020Quote: ... The signal intensity from the examined proteins were evaluated on free-floating sections using the standard method of simultaneously combined staining (Abcam Protocol). Brain slices (50 μm ...