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Citations for abcam :
1751 - 1800 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were analyzed via ICC for the expression of smooth muscle protein 22-alpha (SM22A, Abcam, Cambridge, UK), ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2 ...
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bioRxiv - Microbiology 2023Quote: ... The immunohistochemistry technique was performed with a commercial antibody anti-SARS-CoV-2 spike protein (Abcam, Cambridge, UK) and the in-house mAb 1F7 ...
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bioRxiv - Pathology 2023Quote: ... antibodies against the following proteins were used to probe membranes: Collagen type I (0.9 µg/mL, ab138492, abcam), p-SMAD2 (0.1 µg/mL ...
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bioRxiv - Genomics 2023Quote: ... OXPHOS complex component proteins were probed for with an OXPHOS antibody cocktail (1:250, Abcam ab110412, RRID: AB_2847807). Protein levels were determined by band densitometry and quantified by normalizing to the most stable complex component (V).
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bioRxiv - Cancer Biology 2023Quote: ... AHA-labeled newly synthesized proteins were analyzed by electrophoresis and detected with anti-biotin antibody (Cat# ab201341, abcam). For visualizing total proteins ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared products were collected and incubated in fresh Protein A agarose beads and anti-huntingtin EPR5526 (Abcam) for 2 hours rotating at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein samples were separated by SDS-PAGE and Western blots were performed with following antibodies: UCP1 (Abcam, #ab209483), PPARγ (Cell Signaling Techonology ...
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bioRxiv - Molecular Biology 2023Quote: ... the plates (UltraCruz ELISA Plate sc-204463) were coated with purified recombinant human c-Myc protein (Abcam ab84132), diluted in three different concentrations (0.12 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein G Sepharose® 4 Fast Flow beads (Cytiva) bound to 1 µg of anti-TRIB3 (abcam, ab75846) were incubated with 500 µg of cell lysate for 2 hours at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were immunostained with anti-Arl13b (Protein Tech, 17711-1-AP or Abcam, ab136648; both 1:1000), anti-pericentrin (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Protein complexes were blotted on a nitrocellulose membrane and incubated with rabbit anti-ubiquitin primary antibody (Abcam # ab134953) to look at protein ubiquitination in a western blot.
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bioRxiv - Microbiology 2023Quote: ... identical protein lysate aliquots were incubated with mouse monoclonal anti-alpha tubulin antibody ((Abcam Cat# ab15246, RRID: AB_301787) with secondary antibody rabbit anti-Mouse IgG H&L (Abcam Cat# ab6721 ...
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bioRxiv - Biophysics 2024Quote: ... The proteins were transferred to nitrocellulose membrane by capillary methods and probed with anti-CPEB4 antibody (Abcam ab224162) to follow the aggregation of WT nCPEB4 in a time-dependent manner.
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bioRxiv - Molecular Biology 2021Quote: ... we analyzed 10 pmol of the complex to be used in the assay by SDS-PAGE and staining with Instant Blue (Abcam, cat. no. 119211). We measured the band intensity of Ysh1 (for cleavage assays ...
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bioRxiv - Immunology 2021Quote: ... Free fatty acid concentration in plasma (diluted 1:25) of clamped mice was determined using a quantitative Free Fatty Acid Assay Kit (Abcam, Cambridge, Great Britain). Absorbance was measured with the Filter Max F5 device (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... TEER was measured again and antibody extravasation through the endothelial barrier was quantified by enzyme-linked immunosorbent assay (ELISA) (Abcam, cat. no. ab157709) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... felis C4 butanol extract were measured with 2’,7’-dichlorofluorescein diacetate (DCFDA) following the manufacturer’s instructions (DCFDA/H2DCFDA Abcam cellular ROS assay kit). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The CTB signal was detected by combining the RNAScope Multiplex Fluorescent v2 assay with immunofluorescence using a rabbit anti-CTB primary antibody (Abcam, ab34992, 1:200). The integrated RNA protein co-detection workflow was followed using the RNA-Protein Co-detection Ancillary Kit (Advanced Cell Diagnostics ...
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bioRxiv - Molecular Biology 2022Quote: ... The cells were rinsed with phosphate-buffered saline (PBS) and then stained in the culture well using the Adipogenesis Assay Kit (cell-based, Abcam Catalog No. ab133102). The dye was eluted using the kit’s elution buffer and quantified using a microplate reader at 490 nm wavelength ...
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bioRxiv - Immunology 2022Quote: ... media was collected and LDH activity measurement was carried out in 96-well plate (Costar, cat no. 3370) according to LDH assay kit (Abcam, cat. no ab102526). Absorbance measurement at 450 and 570 nm was carried out using microplate reader (BioTek ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were collected after 24 h and the caspase activity was determined using the Caspase-3 Assay Kit (Colorimetric) (Abcam ab39401, Cambridge, UK).
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bioRxiv - Molecular Biology 2021Quote: ... The serum is at the top layer and was collected for subsequent colorimetric analysis of the total cholesterol using the Cholesterol/ Cholesteryl Ester Assay Kit (Abcam, Cambridge, MA, USA) following manufactures protocol.
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bioRxiv - Immunology 2020Quote: ... cell supernatant was removed and evaluated to determine cytokine levels using the FirePlex®-HT assay system (Abcam, Cambridge, MA, USA; described below). To label mitochondria in live cells for Cell Painting Palette 2 ...
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bioRxiv - Cell Biology 2023Quote: The chymotrypsin-like activity of the 20S proteasome was assessed using a commercially available fluorometric proteasome activity assay kit (Abcam, catalog number: ab107921) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2022Quote: Chromatin accessibility in isolated cardiac fibroblasts or whole heart from C57BL/6J mice was measured using the Chromatin Accessibility Assay Kit (Abcam, cat. no. ab185901) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Unless specified below, all reagents were from MilliporeSigma (St. Louis, MO, USA). LDH assay kit (catalog no. ab65393) was from Abcam (Cambridge, MA, USA). P ...
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bioRxiv - Cell Biology 2024Quote: The amount of collagen in liver tissues was determined by measuring the content of hydroxyproline using a Hydroxyproline assay kit (Abcam, Cat#MAK008-1KT) as described by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... and the quantification of intracellular and extracellular total cholesterol and its fractions was measured using the Cholesterol Assay Kit (Abcam, Cambridge, United Kingdom) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: LB samples that were autoclaved or not autoclaved were subjected to quantification of NADH/NAD+ using an NAD+/NADH Colorimetric Assay Kit (ab65348, Abcam, Cambridge, United Kingdom). Autoclaved samples were measured before and after either chronoamperometry or cycle voltammetry treatment ...
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bioRxiv - Biochemistry 2022Quote: ... The DNA was stained with antibodies that recognize IdU and CldU (Abcam Cat#ab6326, BD Cat#347580) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was stained with antibodies that recognize IdU and CldU (Abcam Cat#ab6326, BD Cat#347580) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM EDTA) and 1mg of protein lysate was subjected to IP with 5ug of anti-BRD4 antibody (Abcam, #ab128874) or anti-NFκB p65 antibody (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... For subsequent interrogation of complement protein levels all samples were employed in either the C3 (Abcam ab108822 and 108823), iC3b ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were prepared in 1% donkey serum (in PBS) for the following proteins: GFP (ab13970, Abcam, 1:200), HA-tag (ab9110 ...
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bioRxiv - Cancer Biology 2021Quote: ... When using recombinant proteins such as PSME4 [purified as described in(Toste Rêgo and da Fonseca, 2019)] or PSME1 (AB-206168, abcam) 1 nM of each protein was add to 100 ul final reaction ...
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bioRxiv - Neuroscience 2021Quote: ... [APOE4/E4]-Ki astrocytes were incubated for 24 hours with the pro-inflammatory cocktail and different doses (0.5 and 20 µg/ml) of TAGLN3 as human recombinant protein (Abcam) prior to be analysed ...
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bioRxiv - Biochemistry 2021Quote: ... and the purified protein band was also confirmed by Western blot analysis by using an anti-His antibody (Abcam).
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bioRxiv - Cell Biology 2021Quote: ... Supernatant (1 mg protein) was incubated overnight with rotation at 4°C with either nesprin-2 antibody (Abcam, #ab217057) or anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation was conducted using 50 seedlings for each sample with Dynabeads protein A and anti-myc (Abcam, Cambridge, MA). Western blots using anti-myc (Cell Signaling Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... for 30 min and probed with mAb or pAb at 4°C overnight: anti-NPC proteins mAb (1:2,000, ab24609, abcam); anti-NUP98 pAb (1:2,000 ...
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bioRxiv - Neuroscience 2020Quote: ... and probed with antibodies against rabbit monoclonal growth associated protein-43 (GAP-43; 1:2000; #ab75810, Abcam, Cambridge, MA), mouse monoclonal neurofilament light polypeptide (NF-L ...
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bioRxiv - Biophysics 2020Quote: ... 1 mg Protein A dynabeads (2.8 m) were prepared and loaded with 10 g Anti-GFP antibody (Abcam ab290) or Anti-HaloTag (Promega G9281 ...
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bioRxiv - Biophysics 2020Quote: ... 1 mg Protein A dynabeads (2.8 m) were prepared and loaded with 10 μg Anti-RFP antibody (Abcam ab62341) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: 1 mg Protein G dynabeads (2.8 μm) were prepared and loaded with 10 g Anti-Digoxigenin antibody (Abcam ab6212) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... co-immunoprecipitated proteins were eluted from the magnetic beads by incubating with 10 µL 4X LDS Sample Buffer (Abcam) at 70 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... protein expression was determined by Western blotting using as primary antibodies anti-FLAG or anti-V5 tag antibody (AbCam; 1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... Human NQO-1 (#ab28947) and HO-1 (#ab133064) protein level changes were quantified by using ELISA kits from Abcam.
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bioRxiv - Biophysics 2022Quote: ... The following primary antibodies were used to detect the presence of indicated proteins in this study: SETD3 (ab176582; 1:5,000; Abcam), Actin (A2066 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were analyzed by Western Blot using anti-UBF and anti-Top2a antibodies (sc-9131, Santa Cruz; ab12318, Abcam).
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bioRxiv - Neuroscience 2021Quote: 16 μg of protein extracted from frozen human tissue samples was employed in a hemoglobin sandwich ELISA (Abcam Ab157707). The same lot of this kit was employed for the analysis of all samples and the assay was performed according to the manufacturer’s instructions.