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Citations for abcam :
1951 - 2000 of 2348 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... or recombinant primary rabbit anti-ionised calcium binding adaptor molecule 1 (Iba1) antibody (microglia, 1:1000 in 2% BSA and 0.1% Triton [TBS]; Abcam) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked with 2% BSA and incubated with primary rabbit anti-XBP1 antibody (ab37152, 1:200, Abcam, Cambridge, MA, USA), ATF6 antibody (ab203119 ...
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bioRxiv - Microbiology 2023Quote: ... 2 x 106 parasites were collected and incubated with 200ul FITC conjugated VSG2WT antisera (FITC conjugation kit, Abcam ab102884) (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... recovery aCSF included (R,S)-3-(2-Carboxypiperazin-4-yl)propyl-1-phosphonic acid (CPP, 5 μM; Abcam, ab120160) and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline (NBQX ...
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bioRxiv - Neuroscience 2023Quote: ... pUb (pS65-Ub, Cell Signalling Technologies, 62802S, 1:250) and/or ref(2)P/p62 (Abcam, ab178440, 1:1000), diluted in 1% BSA in 0.3% Triton X-100 PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rehydrated and washed with ddH2O for 2 minutes and mounted using Mounting Medium with DAPI (Abcam, USA), and edges sealed with clear enamel once the mounting medium set.
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bioRxiv - Neuroscience 2023Quote: ... 100□μl of supernatant was saved as “Input.” The remaining supernatant was transferred to a 2-ml round-bottom tube and incubated with 5Lμg/μl of anti-GFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for 1 h ...
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bioRxiv - Cell Biology 2023Quote: Intrinsic contractility was increased by treating 90-100% confluent Caco-2 cell monolayers with 100nM/mL calyculinA (Abcam #ab141784) for 10-15 min at 37°C.
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bioRxiv - Molecular Biology 2022Quote: ... Staining was performed using antibodies against 8-Oxoguanine (8-OG or 8-hydroxy-2’-deoxyguanosine; Abcam, Cambridge, UK, ab206461), F4/80 (Santa Cruz Biotechnology Inc ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice with PBS and lysed with ice-cold lysis buffer containing NP-40 (2%, ab142227, Abcam), Triton X-100 (2%) ...
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bioRxiv - Neuroscience 2023Quote: ... The antibodies used for western blotting are as follows: rabbit α-LRRK2 (MJFF2 c41-2, ab133474, Abcam, 1:300); rabbit α-phospho-Ser935 LRRK2 (ab133450 ...
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bioRxiv - Microbiology 2023Quote: ... ~10% of each sample by volume was set aside as input and 2 μL of antibody HA (Abcam ab9110) was added to the remaining sample and incubated overnight at 4°C with rotation ...
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bioRxiv - Genomics 2023Quote: ... 100Lμl of supernatant was saved as “Input.” The remaining supernatant was transferred to a 2-ml round-bottom tube and incubated with 5Lμg/μl of anti-EGFP antibody (ab290; Abcam) at 4°C with end-over-end rotation for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... Prior to DENV-2 infection cells were pre-treated with 1 µM fatty acid synthase (FAS) inhibitor C75 (Abcam) or DMSO diluent only for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: mRNA was isolated as described above and 1 μg was precipitated with 2 μl anti-m6A antibody (ab151230, Abcam) or normal rabbit IgG (sc-2027 ...
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bioRxiv - Bioengineering 2024Quote: ... sections were incubated with a mouse anti-RUNX2 (runt-related transcription factor 2) primary antibody (1:20 in 2.5% NGS, Cat. No. ab76956; Abcam) and a rabbit anti-Sp7/Osterix (OSTERIX ...
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bioRxiv - Cancer Biology 2024Quote: ... and QH1 antibody (Hybridoma Bank, AB_531829, 1:200),or (2) anti-laminin rabbit polyclonal antibody (Abcam, ab11575, 1:250) and QH1 antibody (Hybridoma Bank ...
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bioRxiv - Neuroscience 2024Quote: ... The AMPA receptor mediated currents were recorded at -70 mV for at least 2 min of stable baseline and then blocked with 6,7-dinitroquinoxaline-2,3(1H,4H)-dione (DNQX, Abcam, ab120169) 40 µM ...
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bioRxiv - Bioengineering 2024Quote: ... No. 30-2300) were purchased from ATCC. 4,6-diamidino-2-phenylindole (DAPI) (Cat. No. ab228549) was purchased from Abcam. EGFP mRNA-lipid nanoparticle (LNP ...
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bioRxiv - Synthetic Biology 2024Quote: ... MSD GOLD 96-well streptavidin SECTOR plate was coated with 2 μg/mL biotinylated anti-C5b-9 antibody (Abcam) and blocked with PBS containing 2% BSA 0.05% Tween 20 (v/v) ...
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bioRxiv - Neuroscience 2024Quote: ... for 3 hrs at room temperature on an orbital shaker before being incubated in the primary antibody (1:1,000, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in 1% non-fat milk in TBST overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... ARGX-119 was detected using a HRP-conjugated goat F(ab’)2 anti-human IgG-Fc polyclonal antibody (Abcam) for 1h at RT followed- by addition of the TMB substrate ...
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bioRxiv - Neuroscience 2024Quote: ... Then primary antibodies were applied in PBS containing 0.3% Triton and 2% NGS: chicken anti-GFP (1:1000; ab13970, Abcam), rabbit anti-RFP (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were rinsed TBS and nuclei were counterstained with 0.5 µg/mL of 4’,6-Diamidino-2-phenylindole (DAPI; Abcam) at RT for 10 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were held at a constant 100 V for 2 h prior to staining with InstantBlue Coomassie Stain (AbCam).
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bioRxiv - Cell Biology 2024Quote: ... membranes were stripped (Thermo; cat# 21059) and re-blotted with anti-alpha-actinin 4 (Abcam EPR2533[2]; rabbit monoclonal) and anti-GFP (Millipore 11811460001 ...
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bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of total proteins as determined by BCA Protein Assay were analyzed by SDS-polyacrylamide gel electrophoresis with antibodies specific to IL-8 (Cat. No. 18672, Abcam, Cambridge, MA) and prostate specific antigen (Cat ...
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bioRxiv - Neuroscience 2021Quote: ... and labelled for amyloid precursor protein (APP) (Chemicon, 1:1000), MAC3 (Pharmingen, 1:400), glial fibrillary acidic protein (GFAP) (Novo Castra, 1:200) or IBA1 (abcam, 1:1000). Images were acquired at 40x magnification using a bright-field light microscope (Zeiss AxioImager Z1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C overnight in the primary antibodies: chicken anti-glial fibrillary protein GFAP (targeting astrocytes, 1:200, Abcam #ab4674, USA), goat anti-ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2021Quote: ... slices were then incubated at 4 °C for 5-7 days in the primary antibodies containing: chicken anti-glial fibrillary acidic protein (GFAP) (targeting astrocytes, 1:200, Abcam #ab4674, USA) and/or and NeuN (targeting nuclei of neurons ...
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bioRxiv - Neuroscience 2021Quote: ... the brain slides were incubated with primary antibody rabbit monoclonal anti-amyloid precursor protein (APP) [clone Y188] (#AB32136, Abcam, USA; 1:500) in blocking solution at 4 °C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... autoradiography recorded overnight using a phospho-imaging system and protein bands detected by Western blot using anti-TRIM25 (ab167154, Abcam, 1:2000).
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bioRxiv - Neuroscience 2020Quote: ... cell lysates were collected in parallel with the supernatants from the same wells and then tested for levels of a housekeeping protein beta-actin (Ab8227, Abcam, RRID: AB_2305186) by western blot ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then fixed with 4% paraformaldehyde and processed for immunofluorescence analysis using GAP SH3 Binding Protein 1 (G3BP1) antibody (green; Abcam, Cambridge, MA) and fluorophore-conjugated secondary antibody ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Astrogliosis was visualized after staining with rabbit Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody at a 1:1000 dilution (Abcam, Cambridge, MA). All samples were analyzed on an inverted epifluorescent microscope (Olympus IX71) ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated over night with primary antibody rabbit-anti-Ca2+/calmodulin-dependent protein kinase II (1:200, #PA5-38239, Thermo Fisher, MA, USA; 1:500, #ab52476, Abcam, UK), rabbit-anti-parvalbumin (1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... and analyzed by western blot using anti-HBP1 (11746-1-AP, Protein Tech Group, 1:500) or anti-ZMYND19 antibody (ab86555, Abcam, 1:500).
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bioRxiv - Neuroscience 2020Quote: Lysates from DRG neurons were run on 4-12% Bis-Tris gradient gels and proteins were transferred to PVDF membranes that were incubated with antibodies to REST (Abcam, 1:1000), using anti-β-actin as a loading control ...
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bioRxiv - Biophysics 2021Quote: ... threonine and tyrosine phosphorylation of the wt and mutant T654A proteins was detected by western blotting using rabbit anti-pT654 (ab75986; Abcam, Cambridge, UK) and rabbit anti-pY1086 antibody (#4407 ...
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bioRxiv - Cell Biology 2022Quote: ... The relative amount of each of these muscle proteins in each lane was normalized to the amount of Histone H3 detected using anti-Histone H3 (abcam, cat. ab1791) at 1:40,000 dilution ...
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bioRxiv - Pathology 2021Quote: ... tissue proteins (20 μg) were separated by 4-20% SDS-PAGE and immunoblots were developed using antibodies against pSMAD3 (Abcam, 1:1000), SMAD3 (Abcam ...
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bioRxiv - Biochemistry 2021Quote: ... The homogenate was clarified by centrifugation at 21,130 x g for 15 minutes before analysis of protein levels in the supernatant by immunoblotting using antibodies including anti-PKA pRIIα (Abcam, RRID: AB_779040), anti-GFP (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... a pair of monoclonal antibodies (clone EPR16588, ab178846; clone EPR16589, ab178847) and a recombinant human protein (ab105593) are available from Abcam (Waltham, MA). Similarly ...
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bioRxiv - Immunology 2021Quote: ... Concentration of circulating LPS-binding protein (LBP) was determined in plasma samples (diluted 1:5) using Mouse LBP ELISA Kit (Abcam, Cambridge, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used for indirect immunofluorescence microscopy at a dilution of 1:300 and mouse monoclonal antibody against nuclear pore complex proteins (Abcam, Cambridge, MA) was used for indirect immunofluorescence microscopy at a dilution of 1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Electroporated cells were seeded in StemMACS iPS-Brew XF with Rho-associated protein kinase (ROCK) inhibitor Y-27632 (10 μM, Abcam, Cambridge, UK) and 1x CloneR (Stemcell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... the supernatant calibrated into the same protein concentration was used for the measurement of oxytocin levels using an ELISA kit (Abcam, lot. 133050), according to the manufacturer’s instructions.
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Apicosome: newly identified cell-type-specific organelle in mouse cochlear and vestibular hair cellsbioRxiv - Neuroscience 2022Quote: The following primary antibodies were used in this study: two rabbit polyclonal antibodies against human fibronectin leucine-rich transmembrane protein 1 (FLRT1) (ab97825 and ab103839; Abcam, Cambridge, UK), rabbit polyclonal anti-human FLRT2 (ab154023 ...
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bioRxiv - Molecular Biology 2022Quote: Nuclear protein isolations from frozen muscle tissue were performed using a commercial kit (Nuclear Extraction Kit, catalog# ab113474; Abcam; Waltham, MA, USA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... 30mg of protein from scraped cells at day N1 and N14 were analyzed with the Cathepsin D Activity assay kit (ab65302; Abcam, Waltham, MA) following the manufacturer’s instructions.