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Citations for abcam :
2101 - 2150 of 2348 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The medium in each well was replaced with HBSS with 10 μM DCF-DA (2’,7’-dichlorofluorescein diacetate; D6883, Abcam) or propidium iodide staining solution (P4864 ...
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bioRxiv - Developmental Biology 2023Quote: EdU (5-ethynyl-2′-deoxyuridine) labeling assay was performed according to the instruction of EdU Assay / EdU Staining Proliferation Kit (ab219801, Abcam). Proliferating cells were labeled by injecting 10mg/kg BW EdU to mice intraperitoneally 2 hours before sacrifice ...
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bioRxiv - Developmental Biology 2023Quote: ... BSA (blocking agent) was applied on slides for 35 minutes followed by overnight incubation with primary antibodies [MMP-2 (Abcam) at a dilution of 1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 2 hours at room temperature with the following primary antibodies: BUB1 (mouse, Abcam, ab54893, 1:400), BUBR1 (rabbit ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in PBS and incubated for 2 hr at room temperature with secondary antibodies 594-donkey-anti-rabbit (1:50; Abcam) and 488-donkey-anti-chicken (1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated m6A-enriched RNA was immunoprecipitated a second time using 2 µg of a second anti-m6A antibody (Abcam ab151230). Final cDNA Libraries were prepared from twice immunoprecipitated RNA and fragmented input total RNA using the Smarter® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio 634411 ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μg of antibodies ChIP-grade (H3K4me2, Abcam ab32356; H3K4me3, Diagenode C15410003; H3, Abcam ab1791; Normal Rabbit IgG, Abcam ab37415) were used per ChIP and were bound to DiaMag protein G-coated magnetic beads (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: The 5-ethynyl-2’-deoxyuridine (EdU) incorporation assay was performed using the EdU Proliferation Assay Kit (iFluor 488) (ab219801, Abcam). Cells were seeded onto coverslips and incubated with 10 μM EdU for 4 h at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam, 1:3000 dilution) and EP1536Y (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... All slides then were incubated at 4°C overnight with a single IF stain of ACE-2 (1:100, ab15348, Abcam) or ACE-2 (1:100 ...
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bioRxiv - Bioengineering 2024Quote: The second subset of islets was evaluated for oxidative stress by 2’,7’-Dichlorodihydrofluorescein diacetate (DCFDA) (Abcam, cat. no. ab113851) staining of reactive oxygen species (ROS) ...
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bioRxiv - Neuroscience 2024Quote: ... Patient IgG4 was detected using 25 ng/mL of an HRP-conjugated goat F(ab’)2 anti-human IgG-Fc polyclonal antibody (Abcam) in assay buffer for one hour at room temperature followed by adding TMB substrate ...
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bioRxiv - Microbiology 2024Quote: Anti-HIV-1 Pr55Gag + p24 + p17 antibody (ab63917) and anti-tubulin antibodies (B-5-1-2) were purchased from Abcam and Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... Cells were permeabilized with 0.1% Triton X-100 / 2% BSA in 1x PBS for 5 min and F-actin was stained by Phalloidin-iFluor488 (1:1,000, Abcam #ab176753) for 1h room temperature prior to slides mounting (Fluoromount/DAPI).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the sections were incubated with primary antibodies SOX 2 Mouse (Sta. Cruz) with a dilution of 1:200 and Ki-67 rabbit (Abcam) with a dilution of 1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min at room temperature and then blocked in a solution of 0.3% PBS-T and 5% normal donkey serum (NDS) for 2 hours before being incubated with primary antibodies (1°Abs included: chicken anti-GFP [1:1000, Abcam, ab13970] ...
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bioRxiv - Cancer Biology 2024Quote: ... Sheared chromatin was diluted to contain 0.16% SDS for antibody binding and incubated overnight at 4 °C with 2 ug anti-H3K27Ac (Abcam, ab4729), anti-H3K4ME3 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibody solution for Aldh1l1 and vimentin staining: 2 mL blocking buffer containing 20 μL anti-Aldh1l1 antibody (Abcam, AB56777) and 20 μL anti-vimentin (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... Assays were performed using the confor-mation-specific α-synuclein antibody MJF-14 (MJFR-14-6-4-2; Abcam, #ab214033) or the total α-synu-clein antibody syn211 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were rotated for 2 hours at 4 °C in 200 μL D-Wash with 1:200 LMNB2 (Abcam, ab8983) followed by a wash step with D-Wash ...
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bioRxiv - Bioengineering 2024Quote: ... The primary antibodies and respective concentrations used in this study are the following: anti-Ki67 (2 µg/mL, ab15580, abcam), anti-p21Cip1/Waf1 (0.48 µg/mL ...
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bioRxiv - Neuroscience 2024Quote: Dot blots were used to confirm immunoreactivity of generated PFFs with a commonly used conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam). 1.5 µl of serially diluted monomer or (un)sonicated PFF samples were loaded onto nitrocellulose membrane (0.45 µm ...
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bioRxiv - Neuroscience 2024Quote: ... brain tissue was quantified by dot blot (described above) using a conformation-specific anti-αSyn antibody ([MJFR-14-6-4-2], Abcam), and diluted PFFs as a standard series ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 hrs at room temperature before incubation with primary antibody (1:500, conformation-specific anti-αSyn antibody [MJFR-14-6-4-2], Abcam) in blocking solution overnight at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 488-conjugated anti-chicken secondary antibody (goat, 2 mg/ml, dilution 1:500; catalog no. ab150169, Abcam) were used for GFlamp2 and ChR2-EYFP.
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bioRxiv - Neuroscience 2024Quote: ... for 2 h and immediately incubated with a rabbit anti-c-Fos antibody (1:2,000; ab190289, abcam, Cambridge, UK; RRID:AB_2737414) at RT overnight (16 h) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 1-2 h at room temperature with the indicated primary antibodies: rabbit anti-lamin B1 (ab16048, Abcam), mouse anti-separase (6h6 ...
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bioRxiv - Biophysics 2024Quote: ... The cells were then washed twice in ice-cold PBS and blocked for 30 minutes in PBS containing 2% donkey serum (ab7475, Abcam), 300 mM glycine (50046 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the dissected intestines were incubated for 2 hours at 25°C in antibodies against GFP (Abcam, Waltham, MA; Cat#: ab6556) and nuclear pore complex (NPC ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed three times with PBS and stained with 2 μg/mL Alexa488-labeled secondary antibody for mouse IgG (ab150077, Abcam) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were blocked in 10% normal goat serum + 5% DMSO in PBT overnight at 4°C then incubated at 4°C for 24h in primary antibodies (1.25 µg/ml E7 anti-β tubulin (DSHB) and 2 µg/ml ab1791 anti-H3 (abcam) in PBT + 10% normal goat serum) ...
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitation of α-tubulin interacting proteins was performed from total cell lysates using α-Tubulin specific antibodies (rabbit anti-α-tubulin, ab52899, Abcam) and Protein A/G Sepharose beads (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... The eluates were boiled with LDS protein loading buffer (Thermo, NP0008) and loaded on NuPAGE for gel electrophoresis and Western blotting with antibody to ANT1 (Abcam, ab102032, 1:3000). In some experiments ...
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bioRxiv - Genetics 2021Quote: ... ran side by side in a Criterion Precast Gradient 4-15% SDS-PAGE gel for western blotting probing of key intracellular organellar markers and proteins of interest: HSP60 (Abcam, cat. number ab46798), Calnexin (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were transferred to 0.2 um nitrocellulose or PVDF membranes and detected with primary antibodies targeting LRRK2 (ab133474, Abcam, RRID: AB_2713963, 1:500), pS935 LRRK2 (ab133450 ...
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bioRxiv - Plant Biology 2021Quote: ... For each ChIP/re-ChIP assay, 20 μg DNA/protein complexes were immunoprecipitated with anti-H3K27me3 (Milipore, Cat No 07-449) and anti-H3K18ac (Abcam, Cat No ab1191) antibodies using the Re-ChIP-IT kit (Active Motif ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were then processed through an ELISA based kit for detecting p65 (NF-κB p65 Transcription Factor Assay Kit, abcam, Cambridge, MA, #ab133112). Absorbance at 450 nm was read with SpectraMax iD3 plate reader (Molecular Devices ...
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bioRxiv - Neuroscience 2020Quote: ... sections were preincubated (1-hour) at RT in a protein blocking solution (Expose Rabbit-Specific HRP/DAB detection IHC Kit, Abcam, Cambridge, UK, ab80437). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Co-labeling for astrocytes was performed using the same anti-glial fibrillary acidic protein antibody described above (GFAP; Abcam, ab53554; 1:250 dilution). Secondary antibody incubation labeling GFAP positive cells was performed for 1 hour at room temperature using Alexa Fluor 488-conjugated Donkey anti-Goat (ThermoFisher ...
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bioRxiv - Physiology 2021Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then probed to determine protein levels using the following antibodies diluted in 5% non-fat milk in 1x TBST: matrix metalloproteinase 9 (MMP9) (Abcam ab38898, 1:1000) and MMP2 (Abcam ab92536 ...
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bioRxiv - Physiology 2020Quote: ... Nuclear sirtuin 1 content and activity was assessed using 10 µg (5 μL) of isolated nuclear protein fractions through a fluorometric assay (Sirt1 Activity Assay Kit, #ab156065, Abcam, Whitby, Ontario, Canada). Another set of samples weighing 5-10 mg were homogenized in RIPA buffer (150 mM sodium chloride ...
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bioRxiv - Microbiology 2020Quote: ... the fixed cells were labeled with primary antibodies including goat anti-major outer-membrane protein (MOMP; Meridian, Memphis, TN) and mouse or rabbit anti-six histidine tag (Genscript, Nanjing, China and Abcam, Cambridge, UK, respectively). For experiments using CRISPR interference ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... while sections of caspase and protein S staining were incubated with goat anti-rabbit IgG secondary antibody conjugated with HRP (Abcam ab6721, 1:1,000). ImmPACT DAB Peroxidase (HRP ...
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bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:250 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Developmental Biology 2023Quote: Expression of Br-Z1 and Blimp-1 proteins was inferred from staining knock-in strains with chicken anti-GFP (Abcam Ab13970 1:500) and an anti-chicken secondary antibody (Invitrogen A-21437 1:1000).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001; 1:500 α-nsp3, abcam ab283958) followed by a 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer quality and total protein were assessed by Ponceau-S staining before the membranes were probed for Myc-tag (Abcam, #ab9106, 1:1000). Fluorophore-conjugated secondary antibodies were used for detection using the Licor Odyssey CLx system (anti-rabbit IgG ...
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bioRxiv - Molecular Biology 2024Quote: ... supernatants containing soluble proteins were resolved on polyacrylamide gel with 10% SDS and analyzed by WB with anti-FBL (Abcam, 1:1,000 dilution), anti-DDX21 (ProteinTech ...