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Citations for abcam :
1751 - 1800 of 2348 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Chromatin immunoprecipitation was performed by incubating the pre-cleared chromatin with 75 μL Dynabeads Protein G bound with 7.5 μg H3K27ac antibody (Abcam ab4729) overnight at 4°C rotating ...
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bioRxiv - Immunology 2021Quote: ... and hLIGHT-6×His tag proteins (made by the methods described above) in the mixtures with anti-6×His tag PE-labeled antibody (Abcam) for 20 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against S14 phosphorylated Syx (Abcam; ab63571), using the ECL detection system (Pierce Protein Research Products ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoprecipitated proteins were separated by SDS-PAGE and subjected to Western blotting with antibody against Syx phospho-S14 (Abcam; ab63571). Digitized scans were derived by PhosphorImager (Molecular Dynamics ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... mCherry was identified based on its auto fluorescent signal while the luciferase protein was immuno-stained with anti-firefly luciferase antibody (Abcam) labeled with Zenon AF647 Mouse Labeling Kit (Life Technology) ...
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bioRxiv - Biochemistry 2021Quote: ... Clones were validated for GPC1/5 knockouts via Western analysis following heparinase III digestion using a monoclonal antibody (3G10) direct against the glycosylation attachment site in the core protein structure (370260, abcam) or via sequencing (GPC1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lgr6 protein levels were analyzed by standard western blot analysis using anti-LGR6 (ab126747, 1:1000; Abcam, Cambridge, MA, USA) and anti-GAPDH (ADI-CSA-335-E ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates containing 200 µg of total protein were incubated with anti-ERK (CST) or anti-DUSP6 (Abcam, Cambridge, UK) antibody at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... The co-immunoprecipitated protein complexes were detected using western blotting with the following antibodies: anti-TCFL5 (1:1000, ab188075, Abcam), anti-FXR1 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), CLIC4 (Santa Cruz) ...
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bioRxiv - Microbiology 2022Quote: The obtained proteins were separated by 12% SDS–PAGE gel and immunoblotted using anti-GFP (1:5000 dilution, Abcam, ab183734), anti-RFP (1:5000 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... and a cocktail of biotinylated detection antibody (~1 ng/ml per each protein panel) and phycoerythrin (PE, 532 nm emission) conjugated anti-human IgG antibody (10 ng/ml, Abcam) was loaded onto the barcoded array slide after removing excess serum using BSA solution ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-G3BP1 beads were generated by incubating 200 μL Protein-G beads with 20 μL anti-G3BP1 antibody (Abcam, ab290) in 1 mL Buffer B (200 mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were run in MOPS buffer at 200 V for 45 minutes and stained with InstantBlue Coomassie protein stain (Abcam). Protein bands intensities were quantified using ImageJ76 and then plotted against fraction numbers using GraphPad Prism version 9.0.1.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The virus titer was quantified by an ELISA assay against the lentiviral envelope protein p24 (HIV1 p24 ELISA Kit, Abcam). The Cas9 expressing MEF cells were infected in presence of 8 µg/ml Polybrene with the lentiviral particles at a multiplicity of infection of 0,3 to avoid multiple gene knock outs in one cell ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were separated by 12 % SDS-PAGE and immunoblotted with anti-GST or anti-His antibody (Abcam, Cambs, England).
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bioRxiv - Microbiology 2023Quote: ... the cells were then incubated with the following primary antibodies in 0.1% BSA solution (1X PBS) overnight in the dark at 4 °C: mouse monoclonal (1:1000) Respiratory Syncytial Virus (F-protein) (Abcam), rabbit monoclonal (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with the polyclonal rabbit anti- myelin basic protein (MBP) antibody (1:200, ab40390, Abcam) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were transferred to PVDF membranes and then immunoblotted with the following antibodies: cleaved Caspase-3 (1:500, Abcam, ab13847), WAVE1 (1:1000 ...
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bioRxiv - Plant Biology 2022Quote: ... The separated proteins were then transferred to a nitrocellulose membrane and blotted with an anti-RFP antibody (Abcam No. 34767) at a dilution of 1/2,000.
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bioRxiv - Neuroscience 2022Quote: ... The expression of ACE2 Receptor protein (Rabbit Anti-Human Polyclonal, Citrate Buffer HIER, dilution 1:2000, Abcam, Code Number: ab15348) and TMPRSS-2 protein (Rabbit Anti-Human Monoclonal ...
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bioRxiv - Physiology 2022Quote: ... Samples were transferred to PVDF membranes that were subsequently probed with the following antibodies for protein and phosphoprotein detection: anti-PIK3CA (ab40776, Abcam), anti-PDPK1 (ab52893 ...
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bioRxiv - Molecular Biology 2022Quote: ... The same samples without BS3 were run as a loading control and stained using a coomassie protein stain (Abcam ab119211) because detection via our anti-DnaD antibody (Eurogentec ...
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bioRxiv - Microbiology 2024Quote: ... Pan-flavivirus envelope protein antibody 4G2 was conjugated to APC using Lightning-Link® APC Conjugation Kit (abcam, Cat# ab201807). Cells were then blocked with Human TruStain FcX in FACS on ice for 10 minutes followed by staining with 4G2-APC in 1x Flow Cytometry Perm Buffer on ice for 20 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-voltage-dependent anion-selective channel protein 1 (VDAC1)/porin (mouse monoclonal, #ab14734, RRID: AB_443084, Abcam; 1:2000 for WB), anti-α-tubulin (rabbit polyclonal ...
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bioRxiv - Immunology 2024Quote: ... Expression of gE protein on cell surface was determined by staining cells with anti-gE mAb (1:200 dilution, Abcam) for 1 hour prior to incubation with PE-anti-human IgG Fc (1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stained for 10 minutes at 37°C with a chicken anti-green fluorescent protein (GFP) antibody (cross-reactive with YFP; ab13970, AbCam) diluted 1:1000 in neurobasal medium to stain for surface GluN2B ...
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bioRxiv - Cell Biology 2024Quote: ... Total 500 μg of cellular extract was precleared with protein A-agarose beads and was incubated with anti-vinculin (Abcam) antibody overnight at 4°C in a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... The purity and size of the protein has been evaluated by Sodium Dodecyl Sulfate Polyacrylamide gel electrophoresis (SDS-PAGE) using RunBlue (#ab270467 Abcam) 4-12% Bis-Tris polyacrylamide gel ...
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bioRxiv - Neuroscience 2023Quote: ... PFC sections were incubated overnight at 4°C in a blocking solution containing primary antibodies against green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam). VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2023Quote: ... VTA sections were incubated overnight in a blocking solution containing antibodies for green fluorescent protein (GFP; 1:1000 dilution, raised in goat, AB6673, Abcam) and tyrosine hydroxylase (TH ...
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bioRxiv - Pathology 2023Quote: ... Five micrograms of extracted protein was used for western blot analysis with primary antibody against MMP2 (1:200; ab92536, Abcam). The signal intensity of bands was quantified using the Image J densitometry software (NIH).
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bioRxiv - Pathology 2023Quote: ... The expression of the MMP2 proteins in cell lysate and culture medium were confirmed by western blot analyses using antibody against MMP2 (ab92536, Abcam).
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bioRxiv - Systems Biology 2023Quote: ... The protein expression and solubility were evaluated by both Coomassie stain and western blot (WB) of the GST and MBP fusion proteins using anti-GST (abcam) and anti-MBP (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of protein A magnetic beads pre adsorbed with 0.5 µg of anti-lamin A/C antibody (Abcam, #ab108595) were added to the protein mixture and incubated for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... pT320-pp1 (1:1000 for the detection of the endogenous protein, 1:30000 for the kinase assay, rabbit, Abcam ab62334), pY15-Cdk1 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: Concentrations of plasma LPS-binding protein (LBP) were examined via Mouse LBP SimpleStep ELISA according to the manufacturer’s protocol (Abcam, #ab269542). Plasma samples were prepared at a 1:16,000 dilution ...
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bioRxiv - Genetics 2023Quote: ... and Atg18 knockdown flies with the expression of mCherry-Atg8a fusion protein were counterstained with diluted CytoPainter Phalloidin-iFlor 488 (from Abcam) for F- actin at room temperature for 15 minutes before the tissues were mounted in phosphate-buffered saline solution (PBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred onto Hybond nitrocellulose membranes (Amersham biosciences) and probed with antibodies specific for alpha smooth muscle actin (Abcam), BACE1 (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein carbonylation was detected by preparing skeletal muscle lysates from approximately 50 mg of muscle by homogenizing in lysis buffer provided by the Protein Carbonyl Content Assay Kit (Abcam). The resulting supernatants were subjected to western blotting following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Gamma-irradiated infected and non-infected tissue specimens were homogenized on ice using Dounce Glass Manual Tissue Grinder (Etcon) following the Plasma Membrane Protein Extraction Kit protocol (Cat# ab65400, Abcam). Total protein concentration was quantified using Pierce™ BCA Protein Assay Kit (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: APOB protein levels in RPE and retinal lysates were measured using an Apo B ELISA Kit (ab230932, Abcam Cambridge, UK). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... The respective concentration of each sample was calculated based on the standard curve generated by known protein concentrations and measured optical density (OD) at 450 nm following background subtraction according to manufacturer’s instructions (Abcam; ab277082).
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bioRxiv - Biochemistry 2023Quote: ... The tri-methylated lysine 9 and 27 analogs on H3K9C/C110A and H3K27C/C110A proteins were confirmed by western blot assays using monoclonal anti-Histone H3K9me3 and H3K27me3 antibody (Abcam).
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bioRxiv - Molecular Biology 2024Quote: ... A total of 3 μg of the peptide was loaded on an SDS–polyacrylamide gel and stained with InstantBlue Coomassie Protein Stain (Abcam). Intensity of the remaining peptide bands were quantified using the ImageJ and plotted.
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bioRxiv - Neuroscience 2024Quote: ... 5mg per sample of lysate was added to 50ul Protein G beads (Cytiva 28951379) pre-bound with 10ul anti-PCB (Abcam 110314 ...
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bioRxiv - Molecular Biology 2024Quote: Protein-nucleosome binding assays were performed by incubating the abovementioned purified Pioneer-seq nucleosome library with human TP53α (Abcam ab84768), TAp63α (Origene TP710041) ...
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bioRxiv - Cell Biology 2024Quote: ... The gels were run in MOPS buffer at 200 V for 45 min and stained with Instant- Blue Coomassie protein stain (Abcam).
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bioRxiv - Cell Biology 2024Quote: ... Lifted cells were washed 2x with cold dPBS (HyClone, SH30264.02) and incubated with lectins at 10 µg/mL or MBL2 protein (Abcam, ab151947) at 1:50 in 3% bovine serum albumin (BSA ...
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bioRxiv - Pathology 2024Quote: ... Each pre-cleared chromatin sample was then incubated with 35μl of protein A/G agarose beads pre-bound with 10μl of ChIP-grade rabbit anti-ESR1 antibody (Abcam, #ab32063) at 4℃ overnight ...