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Citations for abcam :
2151 - 2200 of 2348 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: The lipase activity of recombinant codon-optimized RLip-WT-CO and RLip-S138A-CO proteins was calorimetrically measured using a lipase assay kit (Abcam, Cat-No.: ab102524) following manufacturer’s instruction ...
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bioRxiv - Physiology 2024Quote: ... A commercially available monoclonal antibody was used to detect electron transport chain proteins (rodent OXPHOS Cocktail, ab110413; Abcam, Cambridge, UK, 1:500 dilution), including V-ATP5A (55kDa) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed in 2% formaldehyde in PBS for 10 min at room temperature and stained with Phalloidin-iFluor 594 (abcam, ab176757) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the chromatin fraction was solubilized in EBC-150 buffer with 500 U/mL Benzonase and 2 ug of antibody directed to endogenous RNAPII-S2 (Abcam; ab5095) or RNAPII-S5 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound primary antibody was removed the following day by washing in 0.2% Tween 20 in PBS and blastocysts incubated for 2 hr at RT with goat anti-rabbit Alexa-488 conjugated secondary antibodies (Abcam, ab150077). Following washing ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with secondary antibody for 2 h at room temperature before DAB reaction using the DAB detection kit (Abcam #ab64238). Slides were counterstained with hematoxylin to stain nuclei ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed in PBST as above to remove the excess of the secondary antibody and then one drop of Fluoroshield Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Abcam, #ab104139) was added on the immunostained samples.
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 2 d at 4°C in primary rabbit anti-TH antibody (Abcam, 1:200, in TBSMT + 5% DMSO). After 6 washes x 1 h with TBST ...
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bioRxiv - Neuroscience 2021Quote: ... end stage high- titer viral aliquots or 2) from cell culture media used for rabies virus rescue after PEG- based precipitation (Abcam, ab102538) and quantified using the High Sensitivity RNA ScreenTape assay (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were again washed thrice in 0.1 M PB and then incubated with the secondary antibody for 2 h (goat anti-chicken-IgY conjugated to Alexa Fluor 594, Abcam, ab150172). Following the incubation with the secondary antibody ...
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bioRxiv - Biochemistry 2021Quote: ... for 10 min and then incubated with the following secondary antibody for 2 hrs: goat anti-mouse IRDYe® 680 conjugated antibody (1:10,000; abcam; ab216776). The membrane was washed again with PBST three times for 10 min and imaged on the Odyssey Li-Cor.
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bioRxiv - Neuroscience 2020Quote: ... Each section was then washed three times in PB for 5 minutes each and incubated for 2 hours at 4°C in a 1:500 dilution of Alexa Fluor 488 goat anti-rabbit secondary antibody (Abcam, Ab150077). From this step on ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times with PB and then incubated with the secondary antibody for 2 hours (goat anti chicken Alexa Fluor 594, abcam, ab150172). After incubation with the secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was then incubated for 2 hours at room temperature in a PBTGS secondary antibody solution including goat anti-mouse Alexa568 (1:200; Abcam [ab175473]), goat anti-rabbit Cy2 (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by a 2 hr incubation with a mixture of the secondary antibody (goat anti-mouse Alexa Fluor® 488–conjugated IgG, ab150113 Abcam) and Alexa Fluor 568 conjugated Phalloidin (A12380 ...
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bioRxiv - Cell Biology 2021Quote: ... for 2 hours at room temperature (Chicken anti-GFP, Aves cat#GFP-1020, RRID:AB_10000240; Rabbit anti-NUP153, Abcam cat#ab84872, RRID:AB_1859766), followed by washing 3x 5 min in 1x PBS ...
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bioRxiv - Microbiology 2021Quote: ... Platelets were then permeabilized with 0.25% saponin for 30 min and stained using recombinant anti-Bcl-2 antibodies (AF647, Abcam, Berlin, Germany) for 30 min before being measured by flow cytometry ...
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bioRxiv - Microbiology 2021Quote: ... Gels were either stained with Coomassie brilliant blue or subjected to Western blot using appropriate antibodies: rabbit polyclonal anti-SARS-CoV-2 S antibody (Abcam ab272504) or rabbit polyclonal anti-SARS-CoV/CoV2 M antibody (Novus Biologicals NB100-56569).
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bioRxiv - Molecular Biology 2021Quote: ... cells were permeabilized using 0.1% Triton-X and incubated for 2 hours at room temperature with mouse monoclonal antibody against sarcomeric α-actinin (1:100; Abcam), followed by an incubation of 1 hour with the secondary antibody conjugated to Alexa Fluor-488 (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated with filipin III (1:100) in PBS for 2 h as described in the manufacturer’s protocol of the cholesterol assay kit (Abcam Biochemicals, ab133116). Filipin III was also added in all following incubation steps with the primary and secondary antibody to enable proper staining ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Cell Biology 2021Quote: Total intracellular ROS contents of cultured HCs were measured using the fluorescent probe 2’,7’-dichlorofluorescin diacetate (DCFDA) (ab113851, Abcam, UK) (Lv et al. ...
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bioRxiv - Bioengineering 2022Quote: ... samples were washed x3 times with PBS and incubated for 2 h at room temperature with corresponding secondary antibody (α-mouse. 488, 1:500, ab150077, abcam; α-rabbit ...
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bioRxiv - Microbiology 2022Quote: ... low or no ACE2 were resuspended in FACS buffer (PBS+2% BSA) and incubated for 1 h with 1:500 rabbit anti-ACE2 antibody (Abcam ab272500). Cells were washed with FACS buffer and labeled for 1 h with 1:3000 Alexa-Fluor-488 goat anti-rabbit IgG H&L (Abcam ab150077) ...
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bioRxiv - Cell Biology 2022Quote: ... Epitope detection was conducted with a primary monoclonal mouse antibody against NONO at 1:500 dilution in PBST and a polyclonal rabbit antibody against RNA Pol II phosphorated at Serine 2 (Abcam, ab5095) at 1:500 dilution ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were washed in distilled water and stained for 15 min in 2% w/v Alizarin Red (ab146374, Abcam, Cambridge, UK) in distilled water at a pH of ~4.2 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.7% Triton X-100 in 1X PBS) for 2 hours at room temperature and incubated in blocking solution with anti-GFP antibody (1:500, Abcam, ab6658) overnight at 4 ºC ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated for 1 h with primary antibodies (dilution in 10% goat serum in PBS) to p-Pol 2(S5) (1:1000 dilution; Abcam, #ab5408) and H3K27ac (1:2000 ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies used were α-syn aggregate-specific antibody MJF-14 (rabbit monoclonal MJFR-14-6-4-2, #ab209538, Abcam, 1:25,000) and mouse monoclonal pS129-α-syn (11A5 ...
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bioRxiv - Cell Biology 2021Quote: ... and reacted with an antibody to synaptic vesicle 2 (SV2 1:50; Developmental Studies Hybridoma Bank Ab2315387) and neurofilament 200 (NF200 1:500, Abcam Ab72996) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were blocked and permeabilized with 0.5% TBS-T and 5% BSA and incubated overnight at 4°C with primary antibodies anti-MAP-2 (1:500, Abcam; ab32454) and anti-GFAP (1:500 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sections were rinsed with PBS and blocked for 2 hours at room temperature using 10% normal donkey serum (NDS; Abcam, #ab7475) prepared in 1% BSA in PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated 2 hours at RT in secondary antibody (1:250; in block): AlexaFluor 488 goat α-rabbit (abcam, ab150077) and AlexaFluor 568 goat α-mouse (abcam ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Laminin (1:500, DSHB 3H11), mouse anti-Msx1/2 (1:100, DSHB 4G1) and rabbit anti-RFP (1:500, Abcam ab62341).
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bioRxiv - Bioengineering 2021Quote: ... diluted at 1:100 followed by 2 h of incubation with 1:500 AlexaFluor 568 goat anti-mouse IgG (abcam, UK) diluted at 1:500 ...
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bioRxiv - Biochemistry 2021Quote: ... Standard Western Blot analysis using α-B3GLCT (IHC-plus antihuman B3GALTL antibody, 1:500) and α-rabbit-HRP (2°, 1:5,000, Abcam)] was performed 9 ...
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bioRxiv - Genomics 2021Quote: ... were used to capture 400 μL of SUMO-2/3 antibody (8A2, acquired from Abcam, ab81371; ∼5-10 μg/μL antibody). All washing and handling steps were followed by centrifugation of the beads at 500g for 3 min in a swing-out centrifuge with delayed deceleration and careful aspiration of buffers ...
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bioRxiv - Genetics 2021Quote: ... Cells were incubated with primary antibody (1:400 RNAPII [pSer2] Novus Biologicals NB100-1805, 1:400 PCNA [5E6/2] abcam ab70472) overnight at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Baltimore PKD Center), anti-PC-2 monoclonal antibody (3374 CT-1 414, Baltimore PKD Center) and anti-CD31 primary monoclonal antibody (Abcam 7388) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was used to solubilize N-(1-(2′-(4-Isopropoxyphenoxy)-2,5’-bithiazol-5-yl)ethyl)acetamide (“ACC2 inhibitor 1”; ab142090; purchased from Abcam, Cambridge, UK), 5-[1’-(1-cyclopropyl-4-methoxy-3-methylindole-6-carbonyl)-4-oxospiro[3H-chromene-2,4’-piperidine]-6-yl]pyridine-3-carboxylic acid (“ACC2 inhibitor 2” known as MK-407439 ...
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bioRxiv - Cell Biology 2023Quote: ... U-2 OS transiently overexpressing mCherry-Sec61β were prepared for immunofluorescence as described above using a rabbit anti-Rtn4 primary (Abcam; ab47085) with a goat anti-rabbit secondary conjugated to Alexa Fluor 647 (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated for 2 h at 25°C with appropriate secondary florescence labelled antibodies (donkey anti-goat IgG, Alexa fluor 488 (Abcam, ab150129) & donkey anti-rabbit IgG ...
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bioRxiv - Microbiology 2023Quote: ... the cell lysate was split into two equal volumes and combined with 2 μg of rabbit CCT2 specific monoclonal antibody (Abcam, ab92746), 2 µg of rabbit anti-CCT3 (Ray Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were then washed 3 times in 1x PBS for 10 minutes then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 minutes before being washed 3 more times in 1x PBS for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... blocked with 1% normal goat serum (NGS) for 1 hour and incubated overnight with primary recombinant anti-prolactin receptor antibody [EPR7184(2)] (abcam, ab170935) at 1:50 in 1% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... Grids were washed in blocking buffer 5x 2 mins and then incubated in 10 nm colloidal gold-conjugated anti-rabbit secondary antibody (Abcam, ab27234) diluted 1:20 in blocking buffer for 30 min ...
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bioRxiv - Immunology 2023Quote: ... or phenol red-free RPMI-1640 were serially diluted (2-fold) and mixed 1:1 with 0.2 mM nitrocefin (Abcam, Cambridge, UK). Samples were incubated 1–16 h at room temperature and absorbance at 490 nm was read on a SpectraMax M2 plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: ... 5 s at 60% amplitude) in 320 µL of buffer A containing 2 % (v/v) NP-40 (Abcam, Cat. No. ab142227). The aggregated proteins were sedimented at 15,000 x g for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Floating sections were captured onto gelatin slides in serial order from caudal to rostral brainstem and mounted with 4’6’-diamidino-2-phenylindole dihydrochloride (DAPI) (Abcam, Cambridge, MA).
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed three times in PBS for 15 min and incubated in secondary antibody for 2 h at room temperature: anti-mouse IgG (1/1,000, HRP-conjugated, Abcam, No. ab205719), anti-guinea pig IgG (H + L ...