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Citations for abcam :
1501 - 1550 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with respective assay diluents from each kit to 2.5 μg/ml in 50 μL for the GFAP ELISA kit (Abcam ab233621), 50 μg/ml in 50 μL for the S100B ELISA kit (Abcam ab234573) ...
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bioRxiv - Cell Biology 2022Quote: Cell culture supernatant of differentiated Hoxb8 macrophages was collected and the amount of soluble Galectin-3 was quantitatively assessed using the Galectin-3 Mouse SimpleStep enzyme-linked immuno-sorbent assay (ELISA) kit (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: The Dihydroethidium (DHE) assay kit for the measurement of intracellular superoxide and hydrogen peroxide was purchased from Abcam (Cat#ab236206). The MitoSOXTM for the measurement of mitochondria superoxide was purchased from ThermoFisher Scientific (Cat#M36008) ...
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bioRxiv - Cancer Biology 2023Quote: Changes in mitochondrial membrane potential produced by I3C and BNF in the five cancer cell lines was determined with the tetramethylrhodamine ethyl ester (TMRE) Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: We measured mitochondrial membrane potential (MtMP) using the JC-1(tetraethylbenzimidazolylcarbocyanine iodide) mitochondrial membrane potential assay kit (Abcam; Cat #113850) following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: The acetyl-CoA content of the cell culture medium was determined with the Acetyl-CoA Assay Kit (Reference: ab87546, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Serine racemase treatment of serum was performed with the addition of 5 µl of proprietary serine racemase solution from a DL-serine assay kit (Abcam) to 1 ml of serum for 3 h ...
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bioRxiv - Immunology 2023Quote: ... mouse PGE2 ELISA Kit (ab287802and Cytokine & Chemokine 36- Plex Mouse ProcartaPlexTM Panel 1A (EPX360-26092-901) for Luminex assay were obtained from Abcam and Thermofisher respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were lysed in 80 μL of ice-cold buffer from Glutathione Assay Kit (catalog no: ab239709, Abcam). The lysed cells were kept on ice for 10 minutes followed by mixing thoroughly with 20 μL of 5% SSA (sulfosalicylic acid ...
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bioRxiv - Cell Biology 2023Quote: Total cholesterol (TC) and free cholesterol (FC) were determined using the Cholesterol/Cholesteryl Ester Quantitation Assay (Abcam plc., Cambridge, UK), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Following secondary antibodies were used in this assay: Donkey anti-rabbit IgG Alexa Fluor 647 (ab150075, Abcam, diluted 1:1000) for phopho-Dsn1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Pyruvate concentration in the centrifuged extracellular medium samples (5 min 18000 g, 4°C) was measured in microplates using Pyruvate Assay kit (Abcam) or as described in 36.
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bioRxiv - Neuroscience 2023Quote: ... Human temporal cortex samples were lysed in CB Cell Lysis Buffer from the Cathepsin B Activity Assay Kit (Abcam, ab65300). Mouse cortex samples were lysed in RIPA buffer (150 mm sodium chloride ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Blood samples were collected by terminal cardiac puncture and plasma was separated by centrifugation and analysed using a colorimetric assay (ab65390, Abcam) for detection of total plasma cholesterol and non-HDL cholesterol ...
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bioRxiv - Immunology 2024Quote: ... The amount of cholesterol in cells and supernatants was assessed using the cholesterol/cholesteryl ester assay quantification kit (ab65359, abcam) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Cellular ROS was also detected using the DCFDA/H2DCFDA cellular ROS assay kit according to the manufacturer’s instructions (Abcam; ab113851). To analyze cell populations ex vivo ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... that occurred per minute of incubation with each substrate at 37°C by Aspartate Aminotransferase Activity Assay Kit (Abcam ab105135) and the Alanine Transaminase Activity Assay Kit (Abcam ab105134) ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellet was homogenized on ice with 150 µL of cold assay buffer containing 1 X protease inhibitor cocktail (Abcam), and centrifuged at 12,000 x g for 15 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Cancer Biology 2024Quote: Free glycerol was measured in the infranatant fraction of the isolated adipocyte culture using a colorimetric assay kit (ab65337, Abcam) following the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... Mitochondrial membrane potential in C2C12 myotubes was measured in a fluorescence plate reader (535 nm/580 nm (ex./em, respectively) with 50 nM TMRE and following the TMRE Mitochondrial Membrane Potential Assay Kit (ab113852, Abcam) manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: EA.hy926 cells or EZH2 WT/mutant overexpressing HEK-293 treated with NO donor SNP (500 µM) for 2 hours were processed with the help of a Biotin Switch Assay kit (Abcam) using the standard manufacturer protocol ...
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bioRxiv - Microbiology 2023Quote: ... the resulting supernatant fractions were boiled for 10 min to inactivate enzymatic activity and their glycogen content measured using a Glycogen Assay Kit (ab65620, Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... cellular lysates were incubated for 1h with LND or vehicle with was measured using a hexokinase assay kit (Abcam, ab211103) according to manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity within periosteal MSC was quantified using a Cell Counting Kit 8 assay (WST-8 / CCK8; Abcam, Cambridge, UK). Water soluble tetrazolium salts are used to quantify cell viability through the production of formazan dye similar to that of an MTT assay ...
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bioRxiv - Immunology 2024Quote: ... and were assessed in BMDM and RAW264.7 macrophage cells following exposure to 2-AA at the times indicated and by utilizing the ATP Assay kit (cholorimetric/fluorometric) (#ab83355, Abcam) and the PicoProbe AcCoA assay kit (ab87546 ...
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bioRxiv - Bioengineering 2024Quote: ... hADSCs cultured on a thin layer of hydrogel for 21 days were subjected to Dimethylmethylene Blue Assay (DMMB, ABCAM, ab289846) based on the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 2 ml of cells were harvested by centrifugation and treated according to the standard protocol (TUNEL Assay Kit – FITC, ab66108, Abcam). Measurement of FITC fluorescence was performed with CytoFLEX cytometer (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... Equal micrograms of protein extracts were mixed with 2 µg of Rabbit anti-mCherry antibody (Abcam) for 6 h at 4°C under end-over-end rotation ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl Protein-A Dynabeads were used with 2.5 µg anti-GFP (rb pAb, Abcam ab290), or anti-mCh (rb pAb ...
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bioRxiv - Microbiology 2019Quote: ... 250 mg of total protein was then used to probe Murine antibody inflammation arrays (Abcam; ab133999) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were tested for expression of the fusion protein by Western blot analysis for ApoA1 (Abcam), ApoM (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... against hCMV immediate early protein 1 and 2 (1 μg/ml; Abcam IE1/IE2 CH160 ab53495), nestin (4 μg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... rabbit anti-oligodendrocyte specific protein (OSP; rabbit anti-OSP antibody, 1:100, Abcam, Ab53041, Cambridge, MA) or rat anti-F4/80 (rat anti-F4/80 antibody ...
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bioRxiv - Immunology 2021Quote: ... Labelling of recombinant proteins was carried out using APC and PE conjugation lightning-link kits (Abcam). When using biotinylated antibodies ...
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bioRxiv - Microbiology 2019Quote: ... or goat anti-human IgG (Licor, USA) or mouse anti-surfactant protein A antibody (abcam, ab51891). Antibodies were diluted 1:50 in 0.1M of phosphate buffer containing 0.1% normal goat serum overnight in a humidified chamber at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... as microglia marker and mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (1:1000, Abcam) as astrocyte marker ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were analyzed with primary antibodies against HSF1 (Spa-901; Enzo) and β-tubulin (ab6046; Abcam). The secondary antibodies were HRP conjugated (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred onto nitrocellulose membranes and incubated with antibodies against GSDMD (1:1000, ab219800, Abcam), GSDME (1:1000 ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were diluted in PBS as follows: anti-Octamer-Binding Protein 4 (OCT4, Abcam, ab184665) 1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies bound to ZIKV envelope protein were detected using HRP conjugate anti-rabbit IgG 7090 (Abcam) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2019Quote: ... and anti-Influenza A Virus M2 protein monoclonal antibody 14C2 from mice (abcam, ab5416, 1:2000). Monoclonal antibodies against HA of Influenza B virus (a 1:1 mixture of clones 6D12 and 1B5m ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins of interest were labeled with the following primary antibodies: NDUFS3 (mouse, abcam, ab110246, 1:100), SDHA (mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... depletion of DDX39B protein was confirmed by western blot using anti-UAP56 (DDX39B) antibody (ab18106, Abcam) and α-Tubulin (AB_1904178 ...
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bioRxiv - Pathology 2021Quote: ... Equal protein loading for immunoblotting was verified by the β-actin blot’s intensity (ab8226, Abcam, USA). Concentration of 8-Isoprostane was assessed by the 8-isoprostane ELISA Kit (516351 ...
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bioRxiv - Microbiology 2020Quote: ... The mouse monoclonal antibody directed against the Influenza A Virus NP Protein (clone C43; ab128193, Abcam) and polyclonal rabbit anti-ZO1 (Tight junctions ...
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bioRxiv - Plant Biology 2021Quote: ... Total proteins were extracted and immunoprecipitated with agarose beads conjugated with anti-GFP antibodies (Abcam, #ab1240) as described above ...
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bioRxiv - Cell Biology 2021Quote: ... The protein bands were blotted onto PVDF membrane and probed with anti-Nup155 antibody (abcam EPR17111), which is specific for C-terminus residues (900-1050 aa) ...