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Citations for abcam :
1451 - 1500 of 2076 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: DNA replication sites were detected using EDU Staining Proliferation Kit (iFluor 647, ABCAM # ab222421) on GFP-SET8 full-length transfected COS-7 cells according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Sheared DNA was incubated overnight with anti-histone H3 acetyl K27 antibody (ab4729; Abcam) pre-coupled to protein A/G magnetic beads ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies and dilutions were used: DNA/mtDNA (Abcam, ab27156, 1:1000), LAMP1 (Cell signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Complex I activity was determined by following change in 450 nm absorbance per minute (mOD/min) for 30 minutes following the addition of Assay Solution (Abcam).
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bioRxiv - Molecular Biology 2021Quote: ... to avoid bias by color changes in the medium and inhibition of growth respectively total cellular viability was assayed using the MTS Assay Kit (Abcam). 100% viability was defined as the readout from non-treated cells (addition of PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Pro-inflammatory prostaglandin E2 (PGE2) was also analyzed from media samples (n=3/condition/cell-type/time-point) via enzyme-linked immunosorbent assay (Abcam) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: Cathepsin D activity (used as a proxy for total lysosomal hydrolase activity) was measured by using Cathepsin D Activity Assay Kit (Abcam). Cells were collected ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed and the level of PKC-specific kinase activity was measured in 30 μg cell lysate using the PKC Kinase Activity Assay Kit (AbCam) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2019Quote: ... 21 Gclc KO and Gclc WT mice were anesthetized and eyes enucleated and immediately assayed using the GSH/GSSG Ratio Detection Assay Kit (Fluorometric-Green) (Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and M2 activated (n = 3) was reported as fold-change from control using a commercial intracellular ROS fluorescence assay kit (Abcam, used according to manufacturer’s instructions) ...
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bioRxiv - Neuroscience 2021Quote: Real time reactive oxygen and/or nitrogen species (ROS/RNS) production was monitored in pericytes exposed to various experimental conditions by using the cellular ROS/RNS assay (Abcam). The assay was conducted following manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated with 10 μM TG100-115 for 3 days and conditioned medium was collected as described above followed by ELISA assay using human IL11 ELISA kit (Abcam).
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bioRxiv - Cancer Biology 2020Quote: The relative MDA concentration in cell or tumor lysates was assessed using a Lipid Peroxidation (MDA) Assay Kit (Abcam, #ab118970) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: The amount of ATP released into the medium was measured with an ATP Assay Kit (Fluorometric, ab83355; Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cytotoxicity and killing potential was measured by LDH amount in the cell supernatant using the LDH-Cytotoxicity Assay Kit (Ab65393, Abcam) following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2022Quote: ... Enzyme activity was assessed on the basis of glutamate production as measured with a glutamate assay kit (Abcam, Cat# 138883). The IC50 value was estimated from biological quadruplicates with a four-parameter logistic model63 and with the use of JMP pro 15 software (version 15.1.0).
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bioRxiv - Molecular Biology 2022Quote: ... terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) staining was performed using TUNEL assay kit (Abcam, ab206386) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... SOD isoenzyme activity was measured according to the manufacturer’s instructions in the SOD activity assay kit (Abcam, cat. n. ab65354).
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bioRxiv - Plant Biology 2021Quote: Quantitative measurement of reactive oxygen species was carried out using DCFDA cellular ROS detection assay kit (Abcam, product number AB113851), and the assay was carried out following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Aconitase activities in cytosolic and mitochondrial fraction of the brain cortex lysates were measured using Aconitase Enzyme Activity Microplate Assay Kit (Abcam) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... early apoptosis was assessed by measuring the levels of phosphatidylserine on the cell surface using the Apoptosis/Necrosis Assay Kit (Abcam) (see Supplemental Methods).
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bioRxiv - Cell Biology 2021Quote: Oxidative stress in male and female PTECs was assessed by measuring the intracellular levels of superoxide ion with the Cellular ROS Assay Kit (Red) (Abcam) following the manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... nuclear extracts were prepared from the cells and subjected to NF-κB transcription factor binding assay according to the instructions of the manufacturer (Abcam).
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bioRxiv - Cell Biology 2022Quote: The enzymatic activity of Cathepsin B was measured by using a Cathepsin B Activity Assay Kit (Fluorometric) (Cat. ab65300, abcam). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... The GST beads were washed and eluted for western blot assays using anti-ArpC2 antibody (Abcam, ab96779, 1:3000 dilution). GST ...
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bioRxiv - Cancer Biology 2019Quote: PDH activity was measured on whole cell lysates using the Pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902, Abcam).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... nuclear extracts were prepared from the cells and subjected to NF-κB transcription factor binding assay according to the instructions of the manufacturer (Abcam).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... PAI-1 standard and each plasma sample were used in duplicate for PAI-1 assays using a mouse PAI-1 ELISA kit (cat no ab197752, Abcam).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 50 μl of TAT standard and each sample were used in duplicate for TAT assays using a mouse ELISA kit for TAT (cat no ab137994, Abcam). Similarly ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted and miRNA levels of miR-84 and let-7a were quantified by FirePlex miRNA assay (Abcam) with customized C.elegans miRNA panel following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Cytokine levels in the cellular supernatant were evaluated using the FirePlex-HT assay system with the Human Cytokines FirePlex-HT Panel 1 (ab234897; Abcam). FirePlex-HT immunoassays quantify up to 10 protein analytes per sample from low sample inputs in 384-well plate format ...
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bioRxiv - Immunology 2021Quote: ... NOS production was measured indirectly by Griess reagent in samples using Nitric Oxide Synthase (NOS) Activity Assay (Abcam, Cambridge, UK) with the addition of Protease Inhibitor Cocktail (Abcam) ...
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bioRxiv - Immunology 2021Quote: Glyceraldehyde 3 Phosphate Dehydrogenase Activity was measured by using Glyceraldehyde 3 Phosphate Dehydrogenase Activity Assay kit (Colorimetric) (Abcam®, #ab204732) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... nuclear extracts were prepared from the cells and subjected to NF-κB transcription factor binding assay according to the instructions of the manufacturer (Abcam).
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bioRxiv - Bioengineering 2021Quote: ... 7′-Dichlorofluorescin diacetate (DCFDA) was used to measure intracellular ROS using the DCFDA Cellular ROS Detection Assay Kit (ab113851, Abcam) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The brain ATP level was measured through an ATP Assay Kit according to the manufacturer’s instructions (Abcam, Catalog No. ab83355)10 ...
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bioRxiv - Neuroscience 2021Quote: Plasma samples were used to determine triglycerides levels with the TG assay kit for quantification (Abcam, ab65336; Cambridge, MA, USA) as previously described (Kalyan-Masih et al. ...
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bioRxiv - Biochemistry 2022Quote: ... The assay was revealed by a 1/2000 diluted goat anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (Abcam, UK). All steps were followed by 5 washes with 50 mM pH 7.5 buffer with 0.05 % tween-20 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... an aliquot of the reaction was used to determine the amount of synthesized ATP using ATP Assay Kit (Colorimetric/Fluorometric) (Abcam). The assay was performed at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Cells were washed twice with PBS and subject to SA-β-Gal assay according to the manufacturer’s protocol (Abcam; ab65351). On the next day ...
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bioRxiv - Systems Biology 2022Quote: ... Protein Assay Kit (Thermo-Fisher) and the activation of the c-Jun transcription factor was assayed by c-Jun Transcription Factor Assay Kit (Abcam) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... Cell viability was assessed after 72 hours using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay kit (Abcam, ab197010) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 45 μL of reaction mixtures were added to 100 μL of glutamate detection reaction mix (Abcam Glutamate Assay Kit, ab83389) in a 96-well plate ...
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bioRxiv - Developmental Biology 2022Quote: ATP synthase activity of wild-type and stat3 mutant zebrafish embryos was measured using the ATP Synthase Specific Activity Microplate Assay Kit (Abcam) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Caspase activation was assayed with the Caspase-3 and Caspase-1 colorimetric assay kits from abcam (ab39401 and ab273268, respectively). At harvest ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were transferred to a 96-well plate and the amount of inorganic phosphate released from the hydrolysis of NTP was determined using Phosphate Assay Kit PiColorLock (Abcam) according to protocol by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... the quantification of changes in mitochondrial membrane potential was determined following the protocol of the TMRE-Mitochondrial Membrane Potential Assay Kit (Abcam). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... and cathepsin D (CatD) endo-peptidase activity was determined using commercial assay kits (Cathepsin B: ab65300, Abcam; Cathepsin D: ab65302, Abcam). Samples were homogenized in Lysis buffer as per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Total nitrite/nitrate was measured as a proxy for nitric oxide concentration using the Nitric Oxide Assay Kit (Abcam, ab65327), with 5 hours of incubation with the enzyme cofactor to permit more than 99% conversion of nitric oxide to nitrite.
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were normalized with respective assay diluents from each kit to 2.5 μg/ml in 50 μL for the GFAP ELISA kit (Abcam ab233621), 50 μg/ml in 50 μL for the S100B ELISA kit (Abcam ab234573) ...