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Citations for Promega :
451 - 500 of 2350 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 10X Primer extension buffer and 2 U AMV Reverse Transcriptase (Promega) were added to the annealing mixture and incubated for 1 hour at 42 °C ...
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bioRxiv - Plant Biology 2024Quote: ... according to the instructions: stock oligo(dT)15 primer (C1101, Promega) concentration was 100 ng/μl ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Biophysics 2022Quote: ... Two PCR reactions using GoTaq G2 PCR mix (Promega) and a pUC19 template were set up ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using PCR cleanup kit (Promega). Sanger sequencing was performed at Psomagen ...
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bioRxiv - Cell Biology 2022Quote: ... qPCR reactions were set up in triplicates with GoTaq® qPCR Master Mix (Promega, United States) and run in a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Molecular Biology 2019Quote: ... and set at room temperature for 30 min prior to reading with a GloMax luminometer (Promega). Integration time was set at 1 second 54,55.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed with an RT cDNA Synthesis Kit (Promega). Real-time PCR was performed with SYBR green SuperMix using an iCycler real-time PCR detection system (Bio-Rad).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generatioplan of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and E SgRNA standards have been outlined previously.20 Both PCR products were serially diluted to produce standard curves in the range of 5 × 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences19 ...
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bioRxiv - Neuroscience 2021Quote: ... and reverse transcribed (RT) using the Reverse Transcription System kit (Promega). The resulting cDNA samples were amplified by TaqMan quantitative PCR using commercially available primer/probe kits from Applied Biosystems for BDNF (Gene Expression Assay Mm00432069_m1 ...
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bioRxiv - Genetics 2020Quote: RNA was reverse-transcribed using the GoScript RT kit (Promega A5000) following the kit protocol ...
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bioRxiv - Genetics 2019Quote: ... Reverse-transcription (RT) was performed using the MMLV retro-transcriptase (Promega) from 500 ng of total RNA in a final volume of 60 µl ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μl reverse transcription enzyme M-MLV-RT (Promega, USA). PCR was performed using each specific primer set in a total volume of 20 μl containing 10 pmol of each primer ...
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bioRxiv - Microbiology 2022Quote: ... treatment and cDNA was synthesized using M-MLV RT (Promega, USA). Gene specific primers used (Table 4) ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription (RT) was performed with M-MLV enzyme kit (Promega) and qPCR was conducted with the Syber GreenER qPCR Super Mix Universal (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... digested overnight at RT with 500 ng of modified trypsin (Promega) in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and sgE RNA standards have been outlined previously.31 Both PCR products were serially diluted to produce standard curves in the range of 5 x 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences,30 and were used at 300 nM and 200 nM ...
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bioRxiv - Physiology 2023Quote: ... cDNA was synthesized using Oligo-dTs and M-MLV RT (Promega). qRT-PCR was performed with the StepOnePlus System (Applied Biosystems ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: ... qPCR was performed using GoTaq 1-Step RT-qPCR (Promega, A6020) reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) was utilized to perform cDNA synthesis and qPCR ...
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bioRxiv - Microbiology 2024Quote: ... The reaction consisted of 0.5 µL Luna RT (Promega Cat#E3005S), 2.5 µL of 2X reaction mix ...
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bioRxiv - Microbiology 2024Quote: ... Turbo-treated RNA was then reverse transcribed with AMV RT (Promega) primed with random 9-mer (IDT ...
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bioRxiv - Developmental Biology 2021Quote: ... using primers JZ294/JZ295 (Table S5) and genomic lambda cl857 DNA (Promega) methylated with 5mC CG methyltransferase M ...
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bioRxiv - Cell Biology 2021Quote: ... and processed for reverse transcription using oligo(dT)15 primers (Promega #C110A) and SuperScript III reverse transcriptase (Thermo Fisher Scientific #18080-044) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.2 µM of each primer and 0.5 unit of GoTaq polymerase (Promega) in a total volume of 25 µl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... random primers (C118A) and MTS (G3581) were obtained from Promega (Courtaboeuf, France). QuantiFast® SYBER® Green PCR Kit (204054 ...
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bioRxiv - Neuroscience 2021Quote: 250ng of Total RNA was reverse transcribed with Random Primers (Promega C1181) and MMLV Reverse Transcriptase (Sigma M1302 ...
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bioRxiv - Cell Biology 2021Quote: ... was reverse transcribed using primer OSV 381 with AMV Reverse Transcriptase (Promega). The JEV NS1 gene was PCR-amplified with Deep Vent polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was prepared from RNA (400 ng) by using random primers (Promega), GoScript buffer (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized by using AMV reverse transcriptase with random primers (Promega). TaqMan gene expression assays (Applied Biosystems ...
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bioRxiv - Pathology 2022Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total RNA with 0.5 μg of random primers (Promega) and distilled water up to 10 μl of final volume were incubated at 70°C for 5 minutes and then kept on ice for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... according to the manufacturer’s instructions and using a random primer mix (Promega). The reactions were assembled in triplicates using the qPCRBio SyGreen Mix Lo-Rox Kit (PCRBiosystems ...
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bioRxiv - Immunology 2021Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
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bioRxiv - Genomics 2020Quote: ... total RNA was annealed with 0.1mg/ml oligo(dT)15 primer (Promega) and cDNA synthesis was performed with M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Pathology 2022Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
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bioRxiv - Pathology 2023Quote: ... The final reaction mixtures contained 150 ng random primers (Promega, Madison, WI), 600 nM each primer and 100 nM probe ...
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bioRxiv - Bioengineering 2023Quote: ... mRNA was reverse-transcribed to cDNA by using oligo-dT primer (Promega) and M-MLV reverse transcriptase (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... then cDNA generated using M-MLV reverse transcriptase and random primers (Promega). Taqman (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µM each primer and GoTaq polymerase reagent (Promega, Madison, WI, USA) were subjected to thermal cycling ...
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bioRxiv - Genomics 2023Quote: ... 200 nM primers and one unit of Taq polymerase (M829B, Promega, USA) was used for various fragment amplifications ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table 3.