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Citations for Promega :
301 - 350 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were rehydrated in 60 μL of 20 μg/mL Trypsin (Promega) in 50 mM ammonium bicarbonate solution and incubated for 2h at 55°C ...
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bioRxiv - Molecular Biology 2022Quote: ... SYBR Green I nucleic acid gel stain and Go Taq Hot Start Polymerase (Promega). qRT-PCRs were carried out in a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: In-gel protein digestion of every spot was performed using sequencing-grade trypsin (Promega) as described elsewhere (26) ...
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bioRxiv - Plant Biology 2022Quote: ... and Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) was used to recover the desired bands from the agarose gel ...
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bioRxiv - Microbiology 2023Quote: ... PCR samples were purified by the Wizard SV Gel and PCR Clean system (Promega) following manufacturers’ instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Gel extracted products were ligated into the pGEM-T Easy Vector System I (Promega). Ligation products were transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
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bioRxiv - Cell Biology 2023Quote: ... de-stained and digested in-gel with 200 ng modified trypsin (sequencing grade, Promega) and Rapigest (TM ...
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bioRxiv - Cell Biology 2023Quote: ... gel pieces were dried and rehydrated with 12.5 ng/mL trypsin (Promega, Madison, WI) solution in 25 mM ammonium bicarbonate on ice for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Nanoluciferase activity was assessed using the Nano-Glo In-Gel Detection kit (Promega, USA) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... the blotted proteins were developed using Nano-Glo® In-Gel Detection System (Promega) and scanned with a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Genetics 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in a mixture of 4 ng/µL trypsin (Promega, Madison, WI) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Genomics 2020Quote: ... and in-gel digested overnight at 37°C with sequencing grade trypsin (Promega Cat# V5111). The peptides were then extracted with extraction buffer (50% acetonitrile (Fisher Chemical Cat#A955-4) ...
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bioRxiv - Cancer Biology 2020Quote: ... from the 1.5% TAE gel and cloned into the pGEM- T Easy Vector System (Promega) for transformation ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins from the gel pieces were digested with trypsin HPLC-grade (Promega AG, Dübendorf, Switzerland) overnight and the resulting peptide solutions were desalted on C18-based STAGE tips as previously described [43,44] ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using Wizard SV Gel and PCR Clean-Up System (Promega) according to the instructions by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified product was purified using Wizard SV Gel and PCR Clean-up system (Promega) and inserted into T-Vector pMD20 (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay ...
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bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated to the pGEM-T easy® vector (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with the Wizard SV Gel and PCR Clean-Up system (Promega Switzerland).
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bioRxiv - Genetics 2022Quote: ... The digested minigenes were purified with Wizard SV Gel and PCR Clean-UP System (Promega) and ligated into a BamHI/MluI opened pCAS2 splicing reporter minigene vector (kindly provided by Dr ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega) and forward and reverse sequences were obtained using an Abi 3130XL Genetic Analyzer (Applied Biosystem ...
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bioRxiv - Synthetic Biology 2019Quote: ... The proteins in the dried gel pieces were then digested with 0.1 μg trypsin (Promega) in 20 μl ABC with incubation overnight at 37°C ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were purified using a Wizard SV Gel and PCR Clean up System kit (Promega) and EcoRI/XhoI digested pGREG533 was gel purified using a Qiaex II kit (Qiagen) ...
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bioRxiv - Biophysics 2019Quote: ... or the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Mannheim, Germany). All primers were obtained from biomers.net ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were gel extracted and cloned with the pGEM-T easy Vector system (Promega) and transfected into XL10Gold competent cells (Agilent) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega). The concentrations of amplified PCR products were measured using the Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were in-gel digested with trypsin (Promega, Madison, WI, USA; ratio 1:100) at 37°C overnight after de-staining ...
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bioRxiv - Genomics 2020Quote: ... DNA quality and quantity was confirmed using gel electrophoresis and QuantiFluor dsDNA Sample kit (Promega) as per the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Tandem mass spectrometry was performed on the in-gel digested proteins with trypsin (Promega, Singapore), as described earlier (42) ...
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bioRxiv - Cell Biology 2022Quote: ... Gel bands were washed in 100mM AmBic/ACN prior to adding 1μg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Resulting cDNAs were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and tailing with dATP and TdT ...
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bioRxiv - Microbiology 2022Quote: ... This DNA extract was then gel purified and quantified using a low-mass ladder (Promega). PCR was performed on ∼50 ng of DNA in a reaction mixture containing 1X Takara ExTaq PCR buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and each slab was subjected to in-gel proteolytic digestion with trypsin (Promega, Mannheim, Germany) overnight as described in (Shevchenko et al. ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were alkylated in-gel prior to digestion with 100ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: ... 1.6kb size amplicon was excised and purified using the SV gel clean up kit (Promega) and sent out for sequencing to confirm target ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were digested at 37°C initially with 0.5 µg of trypsin (Promega) per sample for 6 hours then additionally with 0.3 μg of trypsin overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel slices were pre-incubated with 20 μl of pre-activated trypsin (Promega France, Charbonnières) at room temperature for 15-20 min ...
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bioRxiv - Microbiology 2023Quote: Reduced and alkylated proteins were in-gel digested with 100ng trypsin (modified sequencing grade, Promega) overnight at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Developmental Biology 2023Quote: ... then DNA was purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel-purified PCR fragments were cloned into the pGEM-T Easy vector (Promega, cat# A137A) and sequenced using the Sanger’s method.
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified with the Wizard SV Gel and PCR clean up System (Promega, Madison, USA). PCR products were cloned into pGem-T Easy vectors (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... the linearized DNA was purified using the Wizard® SV Gel PCR purification kit (Promega). For the synthesis of 5’-capped mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR cleanup was conducted using the Wizard SV Gel and PCR Clean-Up System (Promega) with elution in 30 μl of double distilled water ...