Labshake search
Citations for Promega :
551 - 600 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... gel pieces were dried and subsequently rehydrated in solution containing 12 ng/μL Trypsin Gold (mass spectrometry grade from Promega), 0.01% ProteaseMAX surfactant (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... while the purification of the PCR fragments was performed using the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
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bioRxiv - Systems Biology 2023Quote: ... and the bands at the predicted product size were excised and purified with a gel and PCR clean-up system (Promega). Sanger sequencing was completed by the Micromon facility at Monash University ...
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bioRxiv - Neuroscience 2023Quote: ... PCR products with desired length were gel purified and cloned into the MluI and NotI sites of the mammalian expression vector pCI (Promega) that contains rho-tag ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplicons were separated by electrophoresis and the corresponding bands were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) kit ...
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bioRxiv - Biochemistry 2023Quote: The gel pieces were then incubated with 60 µL of a 50 mM ammonium bicarbonate solution of trypsin (MS grade, Promega) at 4°C for 90 minutes and incubated at 37°C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... ∼1/3 of the Coomassie-stained band was cleaved from the gel and samples were prepared with Trypsin Gold (Promega) in accordance with manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Neuroscience 2021Quote: The protein lanes were excised from the gels and proteolytic digestion with trypsin/lys-C mix (V5073, Promega, Southampton, United Kingdom) was performed as described (Shevchenko et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... The remaining volume in the tube (40 µL) was purified using the Wizard® SV Gel and PCR Clean-Up System (Promega) following the manufacturers’ protocol ...
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bioRxiv - Genetics 2019Quote: ... the amplicons were purified from an agarose gel using the Wizard SV Genomic DNA Purification System (Promega Corp., Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... The amplified DNA from each potential off-target site was purified using the Wizard ® SV Gel and PCR Clean-Up System (Promega). The concentration of the purified DNA from the potential off-target sites and the template DNA from the positive control was assessed by using a Nanodrop 2000 Spectrophotometer (Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... 3ul of gel extracted PCR product was used for TA cloning with the pGEM®-T Easy Vector System (Promega, A1360) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean-Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega), ligated and subsequently cloned into E ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Amplified fragment isolated by agarose gel band extraction after electrophoresis and cloned into a pGemT easy vector described by the manufacturer (Promega, Germany). Isolated plasmid DNA was sequenced to confirm the identity and orientation of each clone.
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bioRxiv - Neuroscience 2020Quote: ... and bands of expected length were excised and purified using the Wizard® SV Gel and PCR clean-up system (Promega). Sequencing PCR was performed using the purified PCR products ...
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bioRxiv - Neuroscience 2021Quote: ... The 145-160 bp bands (which correspond to inserts of 24-32 nt cDNAs) were extracted and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). The quality of the library was assessed by the Experion DNA 1K chips (Bio-Rad) ...
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bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al ...
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bioRxiv - Immunology 2022Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified by Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial counts were obtained by comparing the signals from the tested samples with a standard curve prepared by serial dilutions of a standard sample purified using the WizardSV Gel and PCR Clean-Up System Kit (Promega, USA) and subsequently cloned into the pGEM-T vector (Promega ...
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bioRxiv - Microbiology 2019Quote: ... were pooled and library cleanup was performed using a Wizard SV Gel and PCR Clean-Up System A9282 (Promega, Madison, WI). The pooled library was submitted to the UW Madison Biotechnology Center (UW-Madison ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR amplicon from the second-round PCR was gel-purified and ligated to the pGEMT- Easy vector (Promega, USA), as per the manufacturer-recommended protocol (Promega ...
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bioRxiv - Molecular Biology 2019Quote: ... after which fragments between 300-350 base pairs (bp) were excised and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). The size selected library was diluted to 12 pM and sequencing was performed using a 600 cycle (paired-end ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were purified using a HiPure Gel Pure DNA Mini Kit (Magen, D2111) and cloned into the pGEM-T Easy Vector (Promega, A1360). For each sample ...
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bioRxiv - Microbiology 2019Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/μl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified using Quantus™ Fluorometer (Promega, USA). The qualified PCR products were mixed ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified PCR products were purified on 1.5% agarose gels and cloned into the pGEM-T vector (Promega Corporation, Madison, WI) for sequence analysis ...
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bioRxiv - Cell Biology 2021Quote: ... YFP-NLS plasmid was digested by BamHI and HindIII and purified by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding UPF1’s NES with HindIII and BamHI overhangs at the end (UPF1-NES wt ...
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bioRxiv - Cell Biology 2021Quote: ... were generated by digesting UPF1-GFP iso2 plasmid with BsrGI and XbaI (both sites located at the very end of GFP) and purifying it by Wizard® SV Gel and PCR Clean-Up System (Promega). Annealed oligonucleotides encoding the NLS of SV40 or NES of snurportin-1 with a stop codon and BsrGI and XbaI overhangs at the end are ligated into the linearised UPF1-GFP iso2 vector (oligonucleotides listed in Table S1) ...
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bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
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bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... whereafter the resulting DNA fragments were separated by gel electrophoresis and the required 5821 bp band was purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). In this linear form ...
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bioRxiv - Cancer Biology 2023Quote: ... Assessment of DNA fragment length was performed by electrophoretic separation in a 0.4% agarose gel with DNA ladder (Promega, WI, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Plant Biology 2023Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gel slices were then dried again and treated with an ice-cold solution of 12.5 ng/μL trypsin (Promega, Madison, WI) in 100 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... Rehydration of the gel pieces was with 50 mM ammonium bicarbonate solution containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4°C ...