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Citations for Promega :
501 - 550 of 636 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... DNA fragments of approximately the correct size were extracted from 1% agarose gels and ligated into the pGEMT-Easy (Promega) subcloning vector ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicon band was cut and purified from the agarose gel before cloning into pGEM-T easy vector (Promega, USA). The plasmids were transformed into E ...
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bioRxiv - Molecular Biology 2020Quote: ... Bands at the molecular weight of HA-RIOK2 were excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega) at 20 ng/μl as previously described (Shevchenko et al. ...
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bioRxiv - Microbiology 2019Quote: ... All DNA extractions were done with the Wizard® SV Gel and PCR Clean Up system kit (Promega, Madison, USA).
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bioRxiv - Genetics 2019Quote: ... Beads were washed four times with IP buffer and bound nucleic acids were isolated using the Wizard SV gel and PCR clean-up kit (Promega). Collected DNA fragments were quantified using RT-PCR as described above with the following primers ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA quality and quantity were assessed using 1% agarose gel electrophoresis and QuantiFluor dsDNA System in a Quantus fluorometer (Promega). NextRAD libraries were constructed by SNPsaurus (SNPsaurus ...
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bioRxiv - Microbiology 2019Quote: ... The linear amplification product was purified using the Promega Wizard SV Gel and PCR Clean-up System (Promega Corporation, A9282), and 4 μL was ligated at 16°C overnight with the T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Wisconsin, USA, A9281) and annealed (denaturation at 95°C for 5 min and then slow cooling of the samples at the rate of -2°C/sec from 95°C to 85°C and then - 0.1°C/sec from 85°C to 25°C for annealing of heteroduplexes ...
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bioRxiv - Biochemistry 2020Quote: ... Gel pieces were cooled on ice and rehydrated with 100 μ L of 10 ng/μL Trypsin Gold (Promega, UK) for 5 min ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was subjected to DpnI digestion and PCR cleanup (Promega Wizard SV Gel and PCR Clean-Up System). 30 ng of PCR amplified pUC19 ...
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bioRxiv - Plant Biology 2020Quote: ... Amplified products were sent to the Australian Genome Research Facility for sequencing to confirm identity following the use of the Wizard SV Gel and PCR Clean-Up System (Promega).
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bioRxiv - Microbiology 2019Quote: ... Products of PCR reactions were purified using the Wizard® SV Gel and PCR Clean-Up system (Promega, Madison, USA). Sequencing reactions were performed using the Big-DyeTM Terminator Version 3.1 Ready Reaction Cycle Sequencing Kit (Applied BioSystems ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 hour at 37°C and DNA was extracted using the Wizard SV gel and PCR cleanup system (Promega). Input and immunoprecipitated DNA was subjected to quantitative PCR using the oligonucleotides shown in Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were then rehydrated in 50 ul 50 mM ammonium bicarbonate containing 4 ng/µl trypsin (Promega, Madison, WI) and 0.01% proteaseMAX (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... The gels were dehydrated with acetonitrile and rehydrated with 10 mM ammonium bicarbonate containing 13 ng µl-1 trypsin (Promega). The protease digestion was carried out for 12 h at 37 °C following which the peptides were extracted from the gels using 1:2 (v/v ...
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bioRxiv - Biochemistry 2022Quote: A large gel piece covering a wide MW range above 100 kDa was cut out and subjected to reduction/alkylation and in-gel digestion with trypsin (Promega) as previously described [19b] ...
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bioRxiv - Neuroscience 2021Quote: ... Visualization of the amplification products was done with ethidium bromide on a 3% electrophoresis gel and sizes were assessed (Bench Top 100bp DNA Ladder; Promega). Negative controls were performed with H2O or by omitting the reverse transcription phase.
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bioRxiv - Microbiology 2022Quote: ... Gel bands were manually excised and cut in pieces before being subjected to digestion using modified trypsin (Promega, sequencing grade) as previously described (51) ...
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bioRxiv - Molecular Biology 2022Quote: SDS-polyacrylamide gel blocks corresponding to VacA fragments identified by parallel VacA immunoblot analysis were excised and subjected to in-gel digestion using trypsin (Promega) or chymotrypsin (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... then excised and gel slices dehydrated with methanol (100mM) ammonium bicarbonate (100mM) mixture before digestion with 200ng of trypsin (Promega) overnight at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and the mitogenome was extracted from the excised band by using the Wizard SV Gel and PCR Clean-up System (Promega).
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bioRxiv - Plant Biology 2020Quote: ... The overlapping PCR products were separated and purified by the Spin Column PCR Product Purification Kit (Wizard SV Gel and PCR Clean-Up System) following manufacturer’s instruction (Promega, WI). Then ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then excised and purified using the Wizard® SV Gel and PCR Clean-Up System kit (Promega, Madison, WI) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... Amplification products were checked on a 1% agarose gel and were purified using the Wizard PCR clean-up kit (Promega). DNA concentration and purity were measured using a ND-1000 UV-Vis Spectrophotometer (Nanodrop Technologies).
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bioRxiv - Biochemistry 2020Quote: ... PCR products were resolved on a 24% TBE gel and visualized using ethidium bromide or cloned into pGEM-T (Promega) and Sanger sequenced ...
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bioRxiv - Microbiology 2019Quote: ... In-gel protein digestion was performed by O/N incubation of the gel pieces with 1 µg of sequencing grade modified trypsin (Promega) in 67 mM ammonium bicarbonate pH 8 at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins from band slices excised from the entire separation profile were in-gel digested at 37°C overnight with 100 ng trypsin (modified sequencing grade, Promega). Supernatants were dried in a vacuum centrifuge and resuspended in 0.1% TFA ...
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bioRxiv - Genetics 2021Quote: ... Bands purification were made with Wizard® SV Gel and PCR Clean-Up System (Promega, 2800 Woods Hollow Road·Madison, USA). Next ...
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bioRxiv - Genetics 2021Quote: ... 3ul of gel extracted PCR product was used for TA cloning with the pGEM-T Easy Vector System (Promega, A1360) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR product was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA), quantified ...
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bioRxiv - Cell Biology 2021Quote: ... DNA fragments containing the T7 promoter sequence were further amplified by PCR and purified using a Wizard SV Gel and PCR clean-up system (#A9281, Promega) to prepare templates for in vitro transcription ...
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bioRxiv - Biochemistry 2022Quote: ... Gel pieces were rehydrated with 50 mM ammonium bicarbonate containing 12.5 ng/µl modified sequencing-grade trypsin (Promega, Madison, WI) at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The gel pieces of the different samples were washed and digested over night at 37 °C with MS-grade trypsin (Promega). MS/MS analysis was done using a previously described setup of a Waters nanoACQUITY gradient UPLC (www.waters.com ...
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bioRxiv - Neuroscience 2022Quote: ... Digestion of proteins in the gel pieces was done by covering them with a trypsin solution containing (8 ng/µL sequencing-grade trypsin (Promega), dissolved in 50 mM NH4HCO3with 10% ACN) ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified on a 1% agarose gel and ligated into a pGEM-T Easy Vector Systems (Promega) plasmid followed by transformation in E ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were visualized using agarose gel electrophoresis and subsequently cloned into a pGEM-T easy cloning vector (Promega, Australia). Blue/white colonies were screened using standard techniques and a test digestion with endonuclease restriction enzyme EcoR1 was carried out to confirm insertion ...
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bioRxiv - Microbiology 2023Quote: ... The gel lanes corresponding to pulsed-SILAC biological triplicates were cut alike into five bands and subjected to a manual in-gel digestion with modified porcine trypsin (Trypsin Gold, Promega). After destaining ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were subjected to in gel tryptic digest overnight at 37 °C with approximately 2 µg trypsin per 100 µl gel volume (Trypsin Gold, mass spectrometry grade, Promega). Peptides were eluted twice with 100 mM NH4HCO3 followed by an additional extraction with 50 mM NH4HCO3 in 50% acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
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bioRxiv - Molecular Biology 2023Quote: ... Reduction, alkylation and in-gel digestion were performed as described (Shevchenko et al., 2006) except that Trypsin/LysC mix (Promega) was used instead of trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... Northern probes were generated from gel-purified PCR products corresponding to the RNAi inserts and radioactively labelled using the Prime-a-Gene labelling system (Promega).
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bioRxiv - Biochemistry 2022Quote: ... PCR products with desired length were gel purified and cloned into the MluI and NotI sites of mammalian expression vector pCI (Promega) that contains rho-tag ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Cell Biology 2023Quote: ... The gel slices were then reduced and alkylated followed by destaining and in-gel digestion using 125 ng Trypsin/LysC (V5072, Promega) as previously described (Shevchenko ...
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bioRxiv - Biochemistry 2023Quote: ... Selected gel bands that did not bind to the 4’-methylgenistein beads were subjected to in-gel digestion with trypsin (MS grade; Promega). Peptide fragments were analyzed using a Q-TOF/MS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...