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Citations for Promega :
201 - 250 of 725 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... the gel lane was cut into 10 bands and the proteins were subjected to in-gel digestion with trypsin (Promega, Madison, WI, United States) as previously described (Leijon et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) after PCR amplification and extrication from the agarose gel using Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The threshold was set to 0.6 ΔRn ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...
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bioRxiv - Microbiology 2020Quote: ... 85 ul of 10% SDS solution and 40 ul of Proteinase K (15mg/ml, MC500B Promega) were added and samples were incubated for 30 minutes at 60° C ...
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bioRxiv - Physiology 2019Quote: ... SDS was removed by chloroform/methanol precipitation and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... In-gel digestion was performed with trypsin (V5280, Promega Corporation) in 50mM NH4HCO3 at 37 °C overnight ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Neuroscience 2021Quote: ... and in-gel digested using modified trypsin (sequencing grade, Promega) as previously described (Salvetti et al. ...
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bioRxiv - Plant Biology 2020Quote: ... or Wizard SV Gel and PCR Clean-Up System (Promega), and then sequenced.
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bioRxiv - Microbiology 2022Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (74) ...
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bioRxiv - Microbiology 2021Quote: ... or the Wizard SV Gel and PCR Cleanup System (Promega) and sent for commercial Sanger sequencing at either GATC ...
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bioRxiv - Microbiology 2021Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade). The resulting peptides were analysed by online nanoliquid chromatography coupled to tandem mass spectrometry (UltiMate 3000 and LTQ-Orbitrap Velos Pro (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Immunology 2021Quote: ... Proteins in gels were digested with sequencing-grade trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Microbiology 2021Quote: ... before being in-gel digested using trypsin (sequencing grade, Promega) as previously described (Casabona et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were in-gel proteolyzed with trypsin gold (Promega) in the presence of 0.01% Protease Max surfactant (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were in-gel digested using Trypsin (Trypsin Gold, Promega), as previous described in Shevchenko et al ...
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bioRxiv - Microbiology 2022Quote: Excised gel bands were digested with sequencing grade trypsin (Promega) and were dehydrated with 1:1 v/v acetonitrile ...
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bioRxiv - Microbiology 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described1 ...
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bioRxiv - Microbiology 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described1 ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Plant Biology 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (Casabona et al. ...
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bioRxiv - Biophysics 2023Quote: ... The gel was then dried and rehydrated with trypsin (Promega) at an estimated 1:20 w/w ratio in 50mM ammonium bicarbonate ...
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bioRxiv - Genetics 2023Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (Casabona et al. ...
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bioRxiv - Biochemistry 2024Quote: ... and in-gel digested using modified trypsin (sequencing grade, Promega) as previously described (75) ...
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (66) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each gel plug was then digested with Trypsin Gold (Promega) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... In-Gel digestion was carried out using Trypsin gold (Promega). The samples were purified using C18 SepPak columns (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were saturated with 250 ng trypsin (Promega) in 50 μL 10 mM ammonium bicarbonate ...
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bioRxiv - Microbiology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, sequencing grade) as previously described (42) ...
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bioRxiv - Neuroscience 2020Quote: cDNA synthesis was performed in a reaction volume of 25 μl (10 cell pools) or 20 μl (single cells) containing dNTPs (0.5 mM, Promega), random primers (100 ng/per tube ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from a single colony of each isolate with a Wizard® Genomic DNA Purification Kit (Promega), and the quantity and quality were determined with a Quantus Fluorometer (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL) (herring sperm DNA, Promega) were added onto the cell pellet ...
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bioRxiv - Developmental Biology 2023Quote: ... The single-guide RNAs (sgRNAs) were generated with the T7 RiboMAX Express Large Scale RNA Production System (Promega, P1320) and purified with the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Synthetic Biology 2023Quote: ... 36 μL 1.0 M lithium acetate (LiAc) and 50 μL single-stranded carrier DNA (2.0 mg/mL, herring sperm DNA, Promega), was added onto the cell pellet ...
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bioRxiv - Genomics 2023Quote: ... NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells collected by LCM (29,50) and control genomic DNA (gDNA) (Promega), sequenced using conventional sequencing methods (13).
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bioRxiv - Developmental Biology 2023Quote: ... Sense and antisense RNA was synthesized in a single reaction using a T7 RiboMAX Express RNAi System (Promega, P1700). After purification ...
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bioRxiv - Cell Biology 2022Quote: ... SDS was removed and proteins were first digested with Lys-C (Wako) and subsequently with Trypsin (Promega) with an enzyme to protein ratio (1:50) ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by the addition of 5.5μL of a mix containing 5% SDS and 0.25M EDTA and deproteinized with proteinase K (Promega) for 1hour at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... SDS was removed according to previous work (52) and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Genetics 2024Quote: ssDNA constructs generated by M13KO7 helper phage were run on a 1% agarose 1X TBE gel at 100 V for 45 min then size selected ssDNA purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega Madison, WI cat no. PR-A9281) and quantified via Nanodrop 6000 (Thermo-Scientific) ...
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bioRxiv - Genetics 2021Quote: ... RNA was visualized by incubating the gel in Diamond Dye (Promega) and the 20-29 nt area of the gel was cut out for purification ...
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bioRxiv - Pathology 2019Quote: ... following manufacturer’s instruction followed gel electrophoresis using 1 kb ladder (Promega). Sequencing reactions were performed by Fisher Scientific or MyGATC ...
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bioRxiv - Cell Biology 2019Quote: ... followed by in-gel digestion with 20ng/μl of trypsin (Promega) for 16-20 h (overnight ...
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bioRxiv - Biochemistry 2020Quote: ... before being in-gel digested using modified trypsin (Promega, sequencing grade) as previously described (Casabona et al. ...
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bioRxiv - Systems Biology 2021Quote: ... purified using Wizard SV Gel and PCR Clean-Up System (Promega), and all 4 reactions were pooled together ...