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Citations for Promega :
151 - 200 of 725 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The vector and digest were both purified using gel extraction using the Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated using T4 ligase (NEB ...
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bioRxiv - Immunology 2022Quote: ... PCR products were run on a 1 % agarose gel and amplicons of the expected size were purified using the Wizard SV Gel and PCR Clean-Up System (Promega, A9282). The samples were submitted to Sanger Sequencing (sequencing service ...
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The resulting PCR products were extracted from 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences) and were quantified by QuantiFluor™-ST (Promega).
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bioRxiv - Microbiology 2022Quote: ... DNA bands at ∼390bp were excised from gel and purified using Wizard™ SV Gel and PCR Cleanup System (Promega A9282) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The DNA gel bands were excised and purified with a Wizard® SV Gel and PCR Clean-Up System (Promega, WI). The final amplicon products were quantified using the Qubit dsDNA HS Assay kit (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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bioRxiv - Plant Biology 2024Quote: ... the bands of desired sizes were gel-purified using Promega Wizard® SV Gel and PCR Clean-Up System (Promega, USA). PCR fragments were assembled into the Universal Level 0 vector (pAGM9121 ...
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bioRxiv - Genetics 2024Quote: ... Resulting amplicons were purified by gel extraction using Wizard SV Gel and PCR Clean-Up System (Promega cat no. PR-A9281) then cloned into TOPO TA pcR2.1 (Invitrogen cat no ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Genetics 2019Quote: ... DNA fragments were separated by agarose-gel electrophoresis and purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, USA). Sequence authenticity was confirmed at the Massey Genome Centre ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... 10 spots were picked from the gel using an Ettan® SpotPicker™ and were in-gel digested with either trypsin (Promega), chymotrypsin (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... All PCR products were checked on 2% agarose gels and then cleaned with the Wizard SV Gel and PCR Clean-up System from Promega (Madison, Wisconsin) and sent off for Sanger-sequencing along with the amplification primers to Molecular Cloning Laboratories (San Francisco ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were extracted from a 2% agarose gel and purified using AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) and quantified by QuantiFluor™-ST (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Reduced and alkylated samples were digested in-gel using 200 μL (or a sufficient volume to cover all the gel pieces) of MS grade trypsin (Promega – Cat.#V5111), diluted to 10 ng/µL in 40 mM TEABC buffer in 1.5-mL proteomics-compatible tubes ...
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bioRxiv - Cancer Biology 2021Quote: ... One μg of RNA was reverse transcribed into single stranded cDNA (Go Script Reverse Transcription System, Promega) and subsequently used for qPCR analyses on a Step One Plus real time PCR detection system (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: Single cell suspensions obtained from tissues or infected cells were lysed in 1X Passive Lysis Buffer (Promega) for 5 min at 37°C and lysates were added to white-bottom 96-well flat-bottom plates (Costar ...
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bioRxiv - Microbiology 2021Quote: ... Synthesis of single strand cDNA was achieved using ImProm-IITM Reverse-Transcriptase (Promega, Madison, WI, United States).
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bioRxiv - Microbiology 2021Quote: ... Cytotoxicity after treatment with single and combined UPR inhibitors was measured using the Cell Titer Blue (Promega) and trypan blue exclusion kits (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Spike+ cells were then single cell sorted into 96-well PCR plates containing RNase inhibitor (N2611; Promega) and stored at −80 °C.
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bioRxiv - Evolutionary Biology 2023Quote: ... aliquots of a single preparation of the basal firefly vector pGL4.23[luc2/minP] (Promega, Genbank Accession # DQ904455.1) were assayed on each experimental day ...
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bioRxiv - Molecular Biology 2019Quote: ... The gel was developed with Furimazime (Promega, cat#N1120) / TBST (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2022Quote: ... Wizard SV Gel and PCR Clean-Up System (ProMega) was used on cDNA samples ...
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bioRxiv - Genetics 2021Quote: ... Gel pieces were saturated with sequencing grade Trypsin (Promega) at a concentration of 10 ng/μl and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Gel slices were subjected to tryptic digest (Promega V5280) overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: In-gel proteolytic cleavage with sequencing grade trypsin (Promega) was performed as described (Shevchenko et al ...
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bioRxiv - Biochemistry 2022Quote: ... In-gel proteolytic cleavage with sequencing grade trypsin (Promega) was performed robotically ...
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bioRxiv - Biochemistry 2022Quote: ... alkylation and then in-gel digestion by trypsin (Promega). Digested peptides purified using a ZipTip C18 (Merck Millipore ...
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bioRxiv - Cancer Biology 2019Quote: ... Gel bands were digested using trypsin/LysC Mix (Promega) overnight and samples were cleaned up using Reversed-Phase ZipTip (Millipore ...
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bioRxiv - Synthetic Biology 2020Quote: ... and purified with the Wizard SV Gel kit (Promega). The purified DNA was incubated with DpnI (New England BioLabs® ...
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bioRxiv - Plant Biology 2019Quote: ... in-gel digestion was performed using trypsin/LysC (Promega) overnight in 25 mM NH4HCO3 at 30 °C ...
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bioRxiv - Microbiology 2022Quote: ... In-gel thiol reduction/alkylation and trypsin Gold (Promega) digestion were performed as in (40) ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were in-gel digested with trypsin (Promega), five fractions per sample ...
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bioRxiv - Microbiology 2023Quote: ... gel-purified and digested with DpnI restriction endonucleases (Promega) before assembly ...
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bioRxiv - Microbiology 2023Quote: ... In-gel digestion was performed using Trypsin Gold (Promega) and ProteaseMAXTM surfactant (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were in-gel digested with trypsin (Promega, V5113). Tryptic peptides were purified by STAGE tips prior to LC-MS/MS measurements ...
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bioRxiv - Plant Biology 2024Quote: ... before in-gel digestion using modified trypsin (Promega, US) as previously described (56) ...
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bioRxiv - Cell Biology 2022Quote: ... Removal of SDS followed by digestion with sequencing grade modified trypsin (Promega Corp., Madison, WS) were performed using the S-Trap microspin column kit as specified by the manufacturer (Protifi,LLC ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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bioRxiv - Cell Biology 2020Quote: ... Single point mutations were introduced into TLNRD1 using site directed mutagenesis with Pfu DNA polymerase (Promega, Madison US), followed by digestion with DpnI at 37°C for 1 hr and transformation into DH10β E ...
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bioRxiv - Cell Biology 2020Quote: ... and biotinylated in a single reaction with the TnT® SP6 Quick Coupled Transcription/Translation System (Promega, L2080) and Transcend™ tRNA (Promega L5016) ...
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bioRxiv - Microbiology 2021Quote: ... Reactions showing a single band at the appropriate molecular weight based on a 100bp ladder (Promega, Madison, WI) used for reference were purified using QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... Renilla luciferase activity and firefly luciferase activity were measured using GloMax-Multi Jr Single-Tube Multimode Reader (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Biochemistry 2022Quote: The single-stranded form of the phagemid pGEM-11Zf+ and the superspiral pGEM-11Zf+ (Promega, Madison, WI, USA) were used as cofactors in ATP hydrolysis activity assays.
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bioRxiv - Genetics 2020Quote: Genomic DNA was extracted from whole single adult mosquitoes using either the Wizard Genomic DNA Purification Kit (Promega) or the DNeasy Blood & Tissue Kit (Qiagen) ...
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bioRxiv - Neuroscience 2022Quote: ... for the synthesis of sense and antisense single-stranded RNAs (ssRNA) with T7 and T3 RNA polymerase (Promega), respectively ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products of the expected size were eluted from the gel and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). The PCR products were then cloned into a pCR2.1-TOPO vector using the Topo TA Cloning kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... PCR bands at the correct fragment sizes were excised from the gel and extracted using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison WI). PCR products were quantified using a qubit 2.0 fluorometer (Invitrogen ...