Labshake search
Citations for Promega :
1 - 50 of 725 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2019Quote: ... after reducing SDS-PAGE on a gradient (4-15%) polyacrylamide gel (Promega) and transfer onto nitrocellulose membrane ...
-
bioRxiv - Microbiology 2021Quote: Protein bands of ~43 kDa were incised from Coomassie stained SDS-PAGE gel (Fig. S1D) and digested in-gel by trypsin according to the manufacturer’s protocol (Promega). Peptides were then extracted and subjected to LC/MS analysis using an Eksigent nano-HPLC connected to the LTQ Orbitrap XL (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... were stacked in the top of a 12% SDS-PAGE resolving gel before in-gel digestion using modified trypsin (sequencing grade, Promega), as previously described (Casabona et al. ...
-
bioRxiv - Molecular Biology 2023Quote: ... The FgTad3-GFP band cut out from the SDS-PAGE gel was digested with trypsin (Promega, USA) in gel at 37°C for 12 hours ...
-
bioRxiv - Microbiology 2019Quote: ... amyloliquefaciens were determined by SDS-PAGE (10% gel) and was compared with the standard protein marker obtained from Promega, USA (Fig ...
-
bioRxiv - Cancer Biology 2019Quote: ... Immune complexes were resolved on the SDS-PAGE and digested with Trypsin Gold (Mass Spectrometry Grade, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were analysed by SDS-PAGE and Western Blot using the Nano-Glo HiBiT blotting system (Promega).
-
bioRxiv - Neuroscience 2020Quote: ... Single bands were excised according to their predicted fragment size and gel-purified (Promega Wizard SV Gel and PCR Clean-Up System ...
-
bioRxiv - Molecular Biology 2020Quote: The protein bands of interest were excised precisely from SDS polyacrylamide gel and subjected to in-gel trypsin digestion using sequencing grade trypsin (Promega, USA). MALDI TOF was performed in HCT Ultra PTM Discovery System (ETD II-Bruker Daltonics ...
-
bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
-
bioRxiv - Plant Biology 2021Quote: ... 0.5% SDS (Promega 10% SDS solution), 0.2 M NaCl ...
-
bioRxiv - Biophysics 2020Quote: ... Slices from SDS gels were digested with 500 ng trypsin solution (10 ng/μL) (Promega, Madison WI) in 50 mM NH4HCO3 and incubated for 4 h at 37°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al ...
-
bioRxiv - Molecular Biology 2022Quote: ... Each single slice containing the whole sample was excised and subjected to in-gel tryptic digestion using modified porcine trypsin (Promega, France) at 10 ng/μl as previously described (Shevchenko A et al. ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.1 % SDS (Promega)) for 24 hours ...
-
bioRxiv - Genetics 2023Quote: ... 0.2% SDS (Promega) and 1 mg/ml Proteinase K (Merck ...
-
bioRxiv - Genomics 2019Quote: ... SDS (10% stock; Promega) was added (0.3% final concentration ...
-
bioRxiv - Microbiology 2022Quote: ... 70µl 10% SDS (Promega V6551) and 5 µl proteinase K (20 mg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... 70µl 10% SDS (Promega V6551) and 5 µl proteinase K (20 mg/mL ...
-
bioRxiv - Genomics 2019Quote: ... 3.5μl of SDS 0.2% (Promega, #V6551) was added to the solution and incubated in a thermal cycler for 7min at 55°C to dissociate the Tn5 that remained bound to the cDNA.
-
bioRxiv - Microbiology 2021Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
-
bioRxiv - Microbiology 2021Quote: ... 70 µL of 10% SDS (Promega V6551) and 5 µL of proteinase K (20 mg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... 1% SDS) containing protease inhibitor cocktail (Promega), Sodium fluoride (10 mM ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... gel-purified (Wizard SV Gel and PCR Clean-Up System, Promega) and blunted using PfuUltra HF DNA polymerase (Agilent Technologies) ...
-
bioRxiv - Biophysics 2023Quote: ... Then the gel was dried using a gel drying kit (Promega) and exposed to a phosphor screen (overnight) ...
-
bioRxiv - Cell Biology 2024Quote: ... Gel pieces were destained and in-gel trypsin digested (Promega, V5280). The peptide mixture was purified on PepClean C18 spin columns (Thermo Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... excised gel segments were subjected to in-gel trypsin (Promega, V5111) digestion and dried ...
-
bioRxiv - Molecular Biology 2022Quote: ... Each lane was sliced into nine gel bands and the excised gel pieces were subjected to in-gel digestion using trypsin (Promega) followed by C18 STAGE tip clean-up (as described in 4).
-
bioRxiv - Molecular Biology 2021Quote: ... Following gel extraction using Wizard SV Gel and PCR CleanUp System (Promega), 500 ng of purified and linearized vector and 200 ng of each specific gene were treated with T4 DNA polymerase enzyme (Fermentas ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... then gel extracted (Promega Wizard SV Gel and PCR Clean-Up System). Linear plasmids were subjected to bisulfite conversion using the EpiMark Bisulfite Conversion Kit (New England Biolabs) ...
-
bioRxiv - Cell Biology 2022Quote: ... and gel slices were subjected to in-gel digestion with Trypsin (Promega) at 37°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... Individual gel piece was subjected to in-gel digestion using trypsin (Promega). The resulting dried peptides were analyzed in technical duplicate on a ThermoFisher Orbitrap Fusion Lumos Tribrid mass spectrometry system equipped with an EASY-nLC 1200 ultrahigh-pressure liquid chromatography system interfaced via a Nanospray Flex nanoelectrospray source ...
-
bioRxiv - Microbiology 2023Quote: ... gel using the Wizzard SV Gel and PCR clean system (Promega, USA) and quantified with Qubit dsDNA HS assay (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... Complexed proteins were eluted in SDS Elution Buffer (Promega) for 30 minutes at room temperature ...
-
bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
-
bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
-
bioRxiv - Microbiology 2019Quote: ... coli strain JM109 single use competent cells were purchased from Promega and used for transformations in E ...
-
bioRxiv - Biochemistry 2021Quote: A single injection of 200 nanograms of K562 digest from Promega was injected on an EasyNLC1200 coupled to a TIMSTOF Flex mass spectrometer linked by a 25cm lonOpticks Aurora nanoLC column ...
-
bioRxiv - Genomics 2019Quote: ... and gel extraction (Wizard™ SV Gel and PCR Cleanup System [Promega A9282]) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... gel pieces were subjected to in-gel digestion with sequencing-grade trypsin (Promega), and the extracted peptides were resolved by reverse phase HPLC ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified by agarose gel electrophoresis and extracted from the gel using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen ...
-
bioRxiv - Microbiology 2023Quote: ... The PCR product was run on a 2% agarose gel using gel electrophoresis and the band was purified with the Wizard® SV Gel and PCR Clean-Up System (Promega, Madison, USA). Samples were submitted to Macrogen Europe (Amsterdam ...
-
bioRxiv - Microbiology 2021Quote: ... and gel bands excised before in-gel digestion using modified trypsin (Promega, sequencing grade). Resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
-
bioRxiv - Cell Biology 2022Quote: Gel bands were subjected to in-gel digestion with sequencing-grade modified trypsin (Promega) as detailed in Belluzzi et al. ...
-
bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... Amplicons were subsequently gel-extracted (Wizard SV Gel and PCR Clean-Up System, Promega), quantified ...
-
bioRxiv - Molecular Biology 2023Quote: ... We first introduced a single FRT site into a pGemT-vector (Promega). Therefore ...
-
bioRxiv - Genomics 2021Quote: ... and the protocol was completed according to manufacturer’s instructions (page 11, Promega #TM050).
-
bioRxiv - Cell Biology 2022Quote: ... The gel slices were reduced and in-gel digested with sequencing grade modified trypsin (Promega). The resulting samples were quenched by 10% trifluoroacetic acid and peptides were extracted and dissolved in 0.1% trifluoroacetic acid ...
-
bioRxiv - Microbiology 2022Quote: ... gel purified using a Wizard® SV gel and PCR clean up system (Promega, Australia) and sequenced using an Illumina MiSeq platform at the Ramaciotti Centre for Genomics ...