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Citations for Promega :
101 - 150 of 725 citations for SDS PAGE Single gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from agarose gel was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... DNA was finally released from chromatin by incubation with 0.4% SDS (Promega #V6551) and 0,5 mg/ml Proteinase K (Thermo Fisher Scientific #AM2546 ...
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bioRxiv - Microbiology 2022Quote: ... Seventy µl of 10% sodium dodecyl sulfate (SDS) (w/v) (Promega, Madison, USA) and proteinase K (final concentration 0.2 mg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... and the desired bands were recovered from the gel using Wizard® SV Gel and PCR Clean-Up System (Promega). Finally ...
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bioRxiv - Microbiology 2019Quote: ... The entire PCR reaction was run on a 2% agarose TAE gel followed by excising the bands and clean-up using Wizard SV Gel Clean-up (Promega). cDNA from WT EEEV and 11337 stocks were used as positive controls during PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Both PCR products were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation [37] ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then digested in-gel by rehydrating the gel slices in trypsin enzyme solution consisting of 6ng/µl trypsin (Promega) in 50mM ammonium bicarbonate solution ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 mM ammonium bicarbonate the gel-pieces were lyophilised and in-gel digestion performed by incubation with sequencing-grade trypsin (Promega) in 10% acetonitrile ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR amplified inserts were run on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR amplicons were gel purified using the Wizard® SV Gel and PCR Clean-Up System (Promega Cat. No. A9281). Next ...
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bioRxiv - Microbiology 2020Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified by means of Wizard SV gel and PCR Clean Up kits (Promega). The amplified gene was treated with Taq polymerase (Fermentas ...
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bioRxiv - Genomics 2019Quote: ... PCR products run on 1% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up System (Promega). Purified PCR products were sequenced and analyzed with the Sequence Scanner Software 2 Version 2 (Applied Biosystems).
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bioRxiv - Cancer Biology 2020Quote: Selected protein spots were excised from the gel and enzymatically digested in-gel essentially as previously described(4) using trypsin (Promega). Gel pieces were washed with 50% acetonitrile to remove SDS ...
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bioRxiv - Biophysics 2020Quote: ... The PCR products and restriction enzyme digestions were purified by agarose gel electrophoresis followed by processing with the Wizard SV Gel and PCR clean up system (Promega). Restriction enzymes were purchased from Fermentas ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The PCR fragments were purified over gel and the DNA was recovered using the Wizard SV Gel and PCR Clean-Up System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Plant Biology 2023Quote: ... Purified DNA was run on 2% agarose gel and bands corresponding to ∼150 bp were cut and purified with a Gel Purification kit (Promega). Libraries were constructed using the Nugen Ovation Ultralow Library System V2 following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2023Quote: ... 2D gels were stained by Coomassie brilliant blue and proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Zoology 2024Quote: ... The amplicons-containing were excised after gel-electrophoresis and extracted DNA by using Wizard SV Gel and PCR Clean-Up System (Promega). The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology ...
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bioRxiv - Microbiology 2024Quote: The denatured protein samples from co-immunoprecipitation were run into stacking gel and the sample gel band stained by Coomassie Brilliant Blue G250 were digested by trypsin (V5280, Promega) with prior reduction and alkylation in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... The resultant PCR products were subsequently subjected to purification through agarose gel electrophoresis employing the Wizard Sv Gel Clean-up system (Promega). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting products were checked on agarose gel (1%) and purified using the Wizard SV Gel and PCR Clean-Up System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... proteins were in-gel digested with trypsin (Promega) and the resulting peptide mixtures were processed on STAGE tips (92 ...
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bioRxiv - Developmental Biology 2020Quote: ... The PCR product was gel extracted (Promega, A9281). Wildtype embryos were injected with a mix containing two sgRNAs (56.25 ng/μl each) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko 37 ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko49 ...
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bioRxiv - Plant Biology 2021Quote: ... In-gel digest was performed with trypsin (Promega) in 50 mM ABC and 5 % ACN at 37 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... In-gel digestion using trypsin (Promega, Madison, WI) was performed as previously described74 ...
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bioRxiv - Biophysics 2022Quote: ... and subsequently purified by gel purification kit (Promega).
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko [46] ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko (Shevchenko et al. ...
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bioRxiv - Cell Biology 2023Quote: ... and in-gel digested with either ArgC (Promega) overnight at 37°C or trypsin (Promega ...
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bioRxiv - Genetics 2023Quote: ... Gels were stained using Diamond DNA stain (Promega) and colloidal blue protein stain to confirm loading ...
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bioRxiv - Biochemistry 2023Quote: ... Gels were stained with Diamond DNA stain (Promega) and the disappearance of the NCP band quantified using ImageLab ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were digested in-gel using trypsin (Promega) according to the method of Shevchenko78 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by in-gel digestion with trypsin (Promega) at 37°C for overnight ...
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bioRxiv - Systems Biology 2019Quote: ... Single cells were collected in 1 μL of cell lysis buffer (1 U RNasein plus [Promega, Madison ...
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bioRxiv - Microbiology 2022Quote: ... Single cycle monolayers were seeded in white bottom plates and read directly for NLuc activity (Promega) on a Synergy HT plate reader (BioTek) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Single cultures were used for DNA extraction using the Wizard® genomic DNA purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The single-stranded DNA generated using this method was purified using PCR purification kit (A9285; Promega) and quantified using Nanodrop ...
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bioRxiv - Genetics 2024Quote: ... Single-stranded cDNA was prepared from the DNase-treated RNA using GoScript Reverse Transcriptase System (Promega).
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were then single cell plated and positive colonies were selected with HaloTag TMR dye (Promega) and verified by PCR of genomic DNA.
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bioRxiv - Cell Biology 2020Quote: ... gel regions were diced and proteins were subjected to in-gel digestion with 6 ng/μL trypsin (Promega, Madison, WI, USA) in 50 mM ammonium bicarbonate at 37 °C for 10-12 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were than extracted from agarose gels and purified using the Wizard® SV Gel and PCR Clean-Up System (Promega). Eluted DNA solutions were then precipitated with ethanol and resuspended in RNase-free water.
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bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
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bioRxiv - Immunology 2021Quote: ... Products were isolated from a 2% agarose gel using the Wizard1 SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany). DNA concentration was determined via the Qubit1 1.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was separated on a 1% agarose gel and purified using the Wizard SV Gel and PCR Clean-Up System (Promega, USA).