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Citations for Promega :
351 - 400 of 1317 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: Human male genomic DNA obtained from Promega (Ref:G1471; Madison, WI) was used to generate the input library ...
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bioRxiv - Biochemistry 2021Quote: ... The residual magnetic beads were then incubated for two hours at 4°C and for one hour at 16°C in 83 microlitres of buffer A200 with 20 Units RNasin (Promega), 12mM DTT and 16 micrograms of TEV-Protease ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were digested using Lys-C (Wako, 1:40, w/w, overnight under gentle shaking at 30°C) and modified trypsin (Promega, 1:60 ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were diluted 1:5 with 100 mM ammonium bicarbonate and digested overnight at 37°C following the addition of 1 µg of Trypsin/Lys-C protease mixture (Promega) and calcium chloride to a concentration of 1 mM ...
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bioRxiv - Microbiology 2019Quote: ... samples were digested with 1mg/ml of RNAseA for 1h at 37°C followed by 15 min inactivation at 72°C and addition of RNAseIN (Promega), or with DNase using the Turbo DNA-free kit (Ambion ...
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bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
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bioRxiv - Genomics 2022Quote: ... samples were diluted 3 times with 50mM Tris buffer (down to 2M final urea concentration) and digested overnight at 37°C using a Trypsin/Lys-C mixture (Promega) at a ratio 1:100 w/w ...
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bioRxiv - Microbiology 2020Quote: ... All samples were digested overnight at 37 °C using a Trypsin/Lys-C Mix (mass spec grade, Promega, Madison, WI) (enzyme/substrate of 1:100 w/w ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted proteins from control HeLa cells with C-terminal mEGFP tag were digested with mass-spectrometry grade Lys-C and trypsin (Promega). Eluted proteins from a cell line lacking full-length Cdc20 (CDC20_M1-fs-M43 ...
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bioRxiv - Cell Biology 2021Quote: ... 50°C for 60 min and 70°C for 15 min) in the presence of 10 fg of Luciferase RNA (Promega) using the SuperSript III reverse transcription kit (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Mtb samples were inactivated at >95°C for 20 min and stored at -20°C until analyzing the ATP levels using the BacTiter Glo assay (Promega) as previously described (19) ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Cancer Biology 2023Quote: ... 56 °C for 30 seconds and 72 °C for 30 seconds (30 cycles) and 72 °C for 5 minutes using GoTaq DNA polymerase (Promega) and a forward primer (hChimera exon 1-F ...
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bioRxiv - Developmental Biology 2023Quote: ... The modified proteins were digested with lysyl endopeptidase (Lys-C) (Wako) for 3h at 37° C and subsequently digested with trypsin (Promega) overnight at 37° C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... in which proteins were reduced (DTT) and alkylated (IAA) before the digestion with Trypsin and Lys-C (Trypsin/Lys-C Mix, Mass Spec Grade Promega) for 14 hours at 37° C ...
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bioRxiv - Neuroscience 2024Quote: ... the bead and protein mixtures were washed twice with 80% ethyl alcohol and once with 100% ACN before being resuspended in 95 µL NH4HCO3 prior to overnight on-bead digestion (enzyme–protein ratio of 1:20) at 1 000 rpm at 37 °C using modified porcine trypsin/Lys-C Mix (Mass Spectrometry Grade, Promega). The digestion was stopped using trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were isolated from the positive clones using the Pure Yield Plasmid Miniprep System (A1222, Promega, USA), and some potential positive plasmids containing the cDNA insert were digested with EcoRI and HindIII to confirm the presence of the IFNε cDNA insert.
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bioRxiv - Plant Biology 2020Quote: ... We found that using rabbit reticulocyte (TnT® T7 Coupled Reticulocyte Lysate System, Recombinant RNasin® Ribonuclease Inhibitor- Promega) instead of wheat germ extract for in vitro transcription and translation of the ABFs significantly reduces the background noise and increased the quality of peaks that are able to be pulled down from DAP-Seq ...
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bioRxiv - Immunology 2022Quote: ... Alkylation was carried out with 10 mM chloroacetamide at room temperature before adding recombinant sequencing-grade trypsin (0.1 μg, Promega). Digestion took place at 37º C for 18 h ...
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... and then digested with trypsin/Lys-C (Promega) for 16 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... alkylated and digested with endoproteinase Lys-C (Promega) followed by modified trypsin (Promega ...
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bioRxiv - Microbiology 2022Quote: ... 400 ng/mL Trypsin/Lys-C (Promega, USA) were added on spin filter and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 0.5 µg of Lys-C / Trypsin mix (Promega) was added ...
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bioRxiv - Immunology 2020Quote: MS was performed on Trypsin/Lys-C (Promega), and Chymotrypsin (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or a combination of trypsin and AspN (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or a combination of Glu-C and AspN (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... a combination of trypsin and Glu-C (Promega), or AspN (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Trypsin/Lys-C mix (Mass Spec Grade, Promega) was added to protein solution at 1:50 ratio (trypsin ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Biochemistry 2023Quote: ... 200 ng of Trypsin/Lys-C mix (Promega) was added ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 μg of trypsin/Lys-C (Promega) on a rotisserie for 18 h at ambient temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 0.2 μg of Trypsin/Lys C (V5071, Promega) was added into each 100⍰μl sample in 50⍰mM ammonium bicarbonate overnight at 37⍰°C ...
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bioRxiv - Biochemistry 2024Quote: ... The proteases trypsin and Lys-C (Promega, V5073) were used to digest the LtEc proteins overnight at 37°C after reduction with 5 mM TCEP at 55°C and alkylation with 20 mM iodoacetamide at room temperature ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Proteins were digested with Lys-C/Trypsin (Promega) at 37 °C overnight with rotation ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
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bioRxiv - Molecular Biology 2019Quote: ... The recombinant plasmids were prepared from some positive clones using the PureYield Plasmid Miniprep System (Cat #A1222, Promega, Madison, USA). The sequencing of the cloned insert was carried out according to Sanger et al ...
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bioRxiv - Biochemistry 2019Quote: The 5’UTRs of each transcript variant were transcribed and purified in vitro as previously described46 from linearized recombinant psiCHECK2 vector (Promega) that contained one of the three UTR sequences downstream of a T7 promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: RNA was isolated from pooled neurons that were stored at −80 in saline solution with 1% (v/v) Recombinant RNasin Ribonuclease Inhibitor (Promega). The RNA was extracted with the Quick-RNA micro prep kit (Zymo ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... The N-glycans were then released using 10 U recombinant Elizabethkingia miricola N-glycosidase F (Promega, V4831, 10 U/μL) for 16 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: CHO K-1 cells overexpressing hPD-L1 and the recombinant TCR ligand (hPD-L1 Antigen Presenting Cells, hPD-L1 aAPCs, Promega) and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...