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Citations for Promega :
501 - 550 of 1317 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St ...
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bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM DTT containing 10 ng/μL trypsin/Lys-C mixture (Promega), and incubated overnight at 25 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then proteolyzed with Trypsin/Lys-C Mix (Promega, V5071) at a 25:1 protein ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Pathology 2020Quote: ... the 60°C annealing/extension temperature recommended by the mastermix manufacturer (Promega) produced more robust amplification (data not shown).
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Biochemistry 2021Quote: ... and finally digested overnight at 37 °C with 375 ng Trypsin (Promega). The peptides were extracted from the gel twice using 100 μl of 50 % acetonitrile and 0.25 % TFA buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteolytic digestion was carried out with a trypsin/Lys-C mixture (Promega) incubated for 18 h at 37°C at a protein to protease ratio of 25:1 ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... the plasmid was re-ligated overnight at 16°C (ligase from Promega) and transform in Stbl4 bacteria ...
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bioRxiv - Microbiology 2020Quote: ... The samples were treated with trypsin/Lys-C protease mix (Promega, V5072) at a protease:protein ratio of 1:25 for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... For digestion Typsin/Lys-C mass spectrometry grade proteases mixture from Promega Corporation was used ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
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bioRxiv - Biophysics 2020Quote: ... Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... which were digested with 1:50 Arg-C protease (Promega, cat. #V1881): histone protein at 37°C for 12-16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Bands digested overnigt at 37 °C with 800 ng of trypsin (Promega) and/or chymotrypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight with Sequencing Grade Modified Trypsin/Lys-C (Promega) at 370C ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 4 ug Trypsin/Lys-C mix (Promega V5073) for 3-4 hrs at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... in-gel digestion was performed using 0.10 µg trypsin/Lys-C (Promega) overnight in 25 mM NH4HCO3 at 30 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Histones were digested in 50 mM ammonium bicarbonate using Arg-C (Promega), and the resulting peptides desalted by C18 StageTip 86 ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were cleaved via trypsin and Lys-C endoproteinase (Promega, Madison, WI), at a ratio of 1:50 enzyme to protein overnight (12-16 hours ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega), and mixed gently at 37°C overnight to digest proteins ...
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bioRxiv - Bioengineering 2023Quote: ... and digested for 2 hours at 50°C using Trypsin Platinum (Promega) using 1:50 Trypsin to sample ratio by mass ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were digested with sequencing grade trypsin/lys C enzyme (Promega) using manufacture recommended protocol ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... samples were digested with 0.1 μg of trypsin/Lys-C mix (Promega) at 37 °C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were digested with sequencing grade Trypsin/Lys-C enzyme (Promega) in the presence of 10% Acetonitrile for 16 hours at 37°C ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... SEP was digested overnight at 37°C with sequencing grade pepsin (Promega) at a 1/20 (pepsin/SEP ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with 0.2 μg trypsin (Promega). Formic acid was then added to 2% and the samples were incubated at 37°C for 30 min to cleave RapiGest ...
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bioRxiv - Neuroscience 2024Quote: ... each well was supplemented with 0.32µg MS grade Trypsin/Lys-C (Promega) in 50 mM NH4HCO3 and incubated overnight at 37 °C within a humidified incubator ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin/Lys-C Mix (mass spectrometry grade) was from Promega (Madison, WI). Glu-1-Fibrinopeptide B was from Waters (Milford ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were digested using trypsin/Lys-C mixture (Mass Spec Grade, Promega) for 16 h according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were digested overnight at 37 °C with 1 μg of trypsin (Promega). Subsequently ...