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Citations for Promega :
451 - 500 of 1317 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Molecular Biology 2020Quote: ... 500-ng of Trypsin/Lys-C Mix (Promega Cat#V5072) was added and allowed to digest overnight for ∼18-hr at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Digestion was performed overnight at 37°C with trypsin (Promega V5113 ...
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bioRxiv - Plant Biology 2020Quote: ... in 50 mM NH4HCO3 or Glu-C (sequencing grade, Promega) in 50 mM Tris/PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Digestion was performed using mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... 50 mM Tris pH 8 overnight at 37 °C (Promega) at an estimated protein:enzyme ratio 1:100 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C with trypsin (Promega) with an enzyme ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl2 and 500 ng Trypsin Lys-C (Promega). On bead digest was carried out overnight at 37°C while shaking at 200 rpm ...
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bioRxiv - Cancer Biology 2022Quote: ... and digested 16 hours at 37°C with trypsin (Promega) and stopped by addition formic acid (FA ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested by Trypsin/Lys-C Mix (Promega). After the digestion ...
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bioRxiv - Molecular Biology 2022Quote: ... firstly with Endoproteinase Lys-C (ratio 1:33 enzyme:lysate) (Promega) for 1 hour at room temperature then with trypsin (ratio 1:33 enzyme:lysate ...
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bioRxiv - Neuroscience 2023Quote: ... In-gel digestion with Trypsin/Lys-C Mix solution (Promega) was performed as previously described (Gonzalez-Lozano & Koopmans ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of 0.1 µg/µL Trypsin/rLys-C (Promega) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing-grade porcine trypsin/Lys-C mix (Promega, Madison, WI) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sequencing-grade endopeptidase Trypsin/Lys-C Mix (Promega) was added at an enzyme–substrate ratio of 1:50 wt/wt ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 25L°C with trypsin (Promega) with an enzyme/substrate ratio of 1:250 ...
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bioRxiv - Physiology 2023Quote: ... and digested (0.4 ug of Lys-C/Trypsin mix, Promega). After 4 hours of digestion in the urea buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Biochemistry 2024Quote: ... and Glu-C (V1651) were obtained from Promega (Finnboda, Sweden).
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bioRxiv - Genetics 2019Quote: ... and digestion were performed by dithiothreitol (DTT, 10mM, 30min at room temperature), iodoacetamide (IAA, 10mM, dark for 30min at 37°C) and trypsin (MS Grade, Promega, 12.5ng/ul, 37°C overnight) sequentially before analyzing by Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Genomics 2022Quote: ... The primers were commercially synthesized (IDT) and tested on human genomic DNA (Promega) to confirm generation of only one amplicon product at the expected size ...
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bioRxiv - Genetics 2020Quote: ... Deidentified healthy donor DNA was obtained from Promega (Human Genomic DNA: Female, G152A). DNA from ALS cases (ND11836 and ND13803 ...
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bioRxiv - Cancer Biology 2020Quote: 2000bp human lncRNA-TANAR(ENST00000425110.1) promoter was cloned into PGL3 basic vectors (Promega). By mutating the crucial site of AR binding site in the lncRNA-TANAR 5’ promoter to EcoRI cutting site (-GAATTC) ...
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bioRxiv - Immunology 2022Quote: Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega). These plasmids were kindly provided by Dr ...
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bioRxiv - Genomics 2022Quote: ... diluted to 1% in purified human genomic DNA (Promega, female, catalog No. G1521). Briefly ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... for 1h at 37°C in presence of random hexamers (Promega). qPCR primers (supplement Table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were purified using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega) using S-Trap (Protifi ...
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bioRxiv - Plant Biology 2021Quote: ... 5 μg/mL Mass Spec Trypsin/Lys-C Mix (V5073, Promega) and 1 mM DTT was added to the samples ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
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bioRxiv - Cell Biology 2021Quote: ... digested overnight with Trypsin/Lys-C (1:20 w/w, Promega), desalted using C18 Hypersep Spin Column (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with mass-spectrometry grade Lys-C and trypsin (Promega). Digested peptides were cleaned up using C18 spin columns (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested with Trypsin/ Lys-C (Promega, WI, USA). According to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... dehydrated and proteolytically cleaved overnight at 37 °C with trypsin (Promega). The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The LgBiT fragment amplified from pBiT1.1-C [TK/LgBiT] Vector (Promega) using KOD One PCR Master Mix (TOYOBO ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
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bioRxiv - Cell Biology 2022Quote: ... in-gel tryptic digestion (250 ng Trypsin-Lys-C mix Promega V5072 in 50 mM ammonium bicarbonate overnight at 37oC) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 hour at 37 °C with RNasin Plus (N2611, Promega). The RNA was extracted with Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
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bioRxiv - Cell Biology 2021Quote: ... They were then digested with mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... stored at −80 °C and analysed using Dual-luciferase substrate (Promega) in a FLUOstar Optima luminometer (BMG labTech) ...