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Citations for Promega :
601 - 650 of 1317 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µg aliquots of Trypsin/Lys-C (MS grade; Promega Corporation, Madison, WI, USA) were dissolved in 400 µl 50 mM TEAB and 20 µg of this solution was added directly onto the resin of the filter (corresponding to 1 µg Trypsin/Lys-C per sample ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed at 42°C by using GoScript Transcription System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested for 18 h at 37°C with 0.5 μg trypsin (Promega). After digestion ...
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bioRxiv - Neuroscience 2019Quote: ... Destined and dried fragments were re-swelled with Trypsin/Lys-C Mix solution (Promega) overnight in a humidified chamber at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins (200 µg) were digested overnight with Lys-C (Wako Chemicals) and trypsin (Promega) using the filter-aided sample preparation protocol29 ...
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bioRxiv - Microbiology 2020Quote: ... and then ligated for 16 h at 4 °C with T4 DNA ligase (Promega). After a further purification step ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were further digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Immunology 2020Quote: ... The proteins were digested with Trypsin Gold overnight at 37°C (Promega, Madison, WI). The resulting peptides were de-salted using C18 SPE cartridges (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... Rat Gβ1 was cloned with a C-terminal SmBiT34 (peptide 86 or HiBiT, Promega) connected with a 15AA polypeptide linker ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were digested overnight at 37°C with Sequencing Grade Modified Trypsin (Promega) and the reaction was stopped by acidification ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized at 42 °C for 60 min with M-MLV-RT (Promega) using gene-specific primer SP1_nbdA ...
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bioRxiv - Microbiology 2020Quote: ... for 1 h at 55°C then with 200 μg/mL proteinase K (Promega) for 1 h at 55°C ...
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bioRxiv - Genomics 2021Quote: ... Samples were then treated for 1 hour at 37°C with RQ1 DNase (Promega) to remove possible contamination by DNA ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested overnight at 37 °C using trypsin (1:50, w/w, Promega). Peptides were extracted in ACN and formic acid and combined extracts were freeze-dried ...
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bioRxiv - Synthetic Biology 2021Quote: ... Samples were digested overnight at 37 °C using Trypsin Gold (Mass Spectrometry Grade, Promega). After digestion ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were then diluted to 4M urea and digested with Lys-C (Promega), 67:1 (protein ...
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Dual and Opposing Roles for the Kinesin-2 Motor, KIF17, in Hedgehog-dependent Cerebellar DevelopmentbioRxiv - Developmental Biology 2022Quote: ... for 15 minutes at 37°C before lysing with reporter lysis buffer (Promega, E397A). Samples were spun at 15,000 rpm for 10 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein digestion was accomplished by overnight incubation at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Biophysics 2022Quote: ... The protein was digested at 37 °C overnight by adding sequencing-grade trypsin (Promega) at a 24:1 substrate-to-enzyme ratio ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then washed in fresh C+Y medium and 1 % NanoGlo substrate (Promega) was added and luminescence was measured 20 times every 1 min in a plate reader (VarioSkan luminometer ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were digested overnight at 37 °C with 1 μg Typsin/LysC mix (Promega) and 0.01% ProteaseMAX (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then digested by Lys-C (Princeton Separations) and trypsin proteases (Promega) (Florens et al. ...
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bioRxiv - Neuroscience 2023Quote: ... proteins were digested overnight at 37°C using MS-grade trypsin (Promega, Cat# V5280). The next morning ...
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bioRxiv - Molecular Biology 2022Quote: ... 2ul/5-10mg DW of Trypsin/Lys-C (Endoproteinase LysC) Mix (Promega, Cat #V5071) was added to samples and incubated with continuous agitation at 150 rpm for overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... Peptides contained C-terminal biotinylations and were spotted on streptavidin-conjugated membranes (Promega #V2861) and imaged with Typhoon FLA 7000 phosphorimager (GE ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested for 18 h with trypsin/Lys-C Mix (Promega Corporation, USA) and 10 μg of each sample was used for proteome analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were digested overnight at 37°C with 10 nG MS Grade Trypsin (Promega). Trypsinization was quenched with 1 μL of 0.1% Trifluoroacetic acid (TFA ...
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bioRxiv - Microbiology 2023Quote: ... Plates were stored at -20°C before adding 100 μl Steady Glo substrate (Promega) to perform a luciferase assay as described earlier ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the assay was performed at 20 °C using a Glomax 20/20 luminometer (Promega) by the luciferin/luciferase system supplemented with 25 µM P1,P5-di(adenosine-5’ ...
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bioRxiv - Systems Biology 2023Quote: ... washing, and digestion with lysyl endopeptidase (Lys-C, Wako) and sequencing-grade trypsin (Promega). The capture and digestion steps were performed with a Versette liquid handling robot (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: N-terminal large (LgBit) and C-terminal small (SmBit) fragments derived from NanoLuc (Promega) were fused to the C-terminus of B56δ and inserted downstream of its N-arm at residue 103 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were incubated at 25°C for 5 days before CellTiter-Glo assays (Promega) were performed ...
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bioRxiv - Cell Biology 2023Quote: ... and cut finely prior to in-gel digestion with Trypsin/Lys-C Mix (Promega) at 37 °C for 12 h ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Biochemistry 2023Quote: Eluted proteins were digested with Lys-C (FUJIFILM Wako) and trypsin (Promega, Madison, WI) according to the PTS protocol21 ...
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bioRxiv - Biochemistry 2023Quote: Tryptic digestion was performed at 37° C with 125 μL Trypsin (Promega Cat# V511X) in digestion buffer (100 mM TEAB pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized from Gene Universal (USA) at 4°C overnight (T4 DNA ligase, Promega, USA). Cys-loop mutation ligation products were transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... The samples were digested overnight at 37°C with sequencing-grade modified trypsin (Promega) at an enzyme to protein ratio of 1:40 ...
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bioRxiv - Synthetic Biology 2023Quote: ... or zero min at 37°C before addition of Nano-Glo substrate (Promega N1110) in 80 µL ...
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bioRxiv - Biochemistry 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... followed by digestion with benzonase at 37 °C for 1 h and trypsin (Promega) digestion overnight at the same temperature ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL of buffer containing 35 mM iodoacetamide and Trypsin/Lys-C (Promega, V5072) was added to each sample ...
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bioRxiv - Cell Biology 2023Quote: ... after which samples were digested overnight at 37 °C with trypsin (Promega, Fitchburg, WI,) in a 1:60 trypsin to protein ratio ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were then diluted to 3M urea and digested with Lys-C (Promega) for 4 h at 25°C ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The NanoLuc coding sequence including linker was obtained from the pNLF1-C plasmid (Promega) and inserted at the C-terminus of the TDO2 gene sequence (Flash Therapeutics ...