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Citations for Promega :
101 - 150 of 4937 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... and cDNA synthesis using the GoScript RT Kit and oligo dT primer (Promega). To amplify the random 5’ and 3’ BC from the plasmid DNA or cDNA sample ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were cleaned up using PCR clean up kit (# A9282, Promega). sgRNAs were synthesized using the MEGAShortScript™ Kit (# AM1354 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Both PCR products were purified using the Wizard PCR cleanup kit (Promega, USA).
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bioRxiv - Zoology 2021Quote: ... PCR products were purified with Wizard SV Gel and PCR Cleanup kit (Promega). Purified products were sequenced at Sequetech DNA Sequence Service (Mountain View ...
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bioRxiv - Systems Biology 2022Quote: ... AMV RT (Promega) with 9 bp random primers was used for reverse transcription ...
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bioRxiv - Microbiology 2023Quote: ... AMV-RT (Promega) was used to reverse-transcribe the RNA-protein complex reaction at 30 °C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... and the 200 nM GoTaq® Probe 1-Step RT-qPCR System Kit (Promega). This kit uses GoTaq Probe qPCR Master Mix with dUTP (10 uL) ...
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bioRxiv - Physiology 2021Quote: ... RT was carried out using M-MLV RT enzyme (Promega #M1705) to obtain cDNA ...
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bioRxiv - Microbiology 2019Quote: ... The RT-PCR fragments corresponding to the LP region were also cloned into a pGem-T easy vector (Promega) and Sanger sequenced.
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was used to amplify the OGDH 3’UTR and sub clone it into pmirGLO vector (Promega, UK). 5 × 10^4 C2C12 cells were seeded into 12 well plates ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the PCR Promega kit (Promega Corporation, WI, États-Unis) following the manufacturer’s instructions and sent to Genewiz company (www.genewiz.com/en-GB ...
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bioRxiv - Microbiology 2021Quote: ... followed by a reverse transcriptase (RT)-step with Moloney Murine Leukemia Virus RT (MMLV-RT, Promega, Madison, WI, USA) in the presence of RNase inhibitor (RNAsin plus ...
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bioRxiv - Immunology 2023Quote: ... Promega GoTaq qRT-PCR kit (Promega, Fitchburg USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... Input linear DNA was produced by PCR and cleaned with PCR cleanup kit (Promega, A6754), quantified and diluted to desired concentrations using ultra-pure water ...
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bioRxiv - Immunology 2024Quote: The PCR products representing genomic and cDNA sequences were purified using PCR purification kit (Promega) and subsequently sequenced using Sanger sequencing at Micromon Genomics ...
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Ligations of the digested plasmid backbones and PCR products occurred for 5-10 minutes at RT using T4 DNA ligase (Promega) prior to transformation into NEB® Turbo Competent E ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Physiology 2019Quote: The expression profile of PKC isoforms in UMR106 cells was studied by RT-PCR using the GoTaq Green Master Mix (Promega) and the primers listed in Table 1 ...
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bioRxiv - Plant Biology 2019Quote: Semi-quantitative RT-PCR was performed on RNA isolated of 10 days-old seedlings using the total RNA isolation system (Promega). Reverse transcription was performed on 300 ng of total RNA using the iScript advanced kit (BioRad) ...
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bioRxiv - Bioengineering 2019Quote: ... a specific BYDV nucleotide sequence (294 bp, primers PVinterF+YanRA) amplified by RT-PCR was inserted into the vector pGem-T Easy (Promega) and cloned into E ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... QRT-PCR reactions were performed by using the GoTaq®1-Step RT-qPCR System (Promega Scientific, Madison, WI, USA) with Bio-Rad CFX Connect (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Each quantitative RT-qPCR reaction was performed using the GoTaq qPCR Master Mix kit (Promega). For a 10 μl reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... which were performed with the use of GoScript RT kit following the manufacturer’s instructions (Promega).
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bioRxiv - Genomics 2021Quote: ... and cDNA synthesis using the GoScript RT kit and oligo dT primer (Promega, Madison, Wisconsin). For STARR-seq samples ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by reverse transcription (RT) using M-MLV reverse transcription kit (Promega, Madison, WI, USA). Blank qPCR Master Mix kit (EURx ...
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bioRxiv - Neuroscience 2024Quote: ... using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega: A6020) in the CFX96 Touch Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2019Quote: ... or AMV RT (Promega) as described in manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: A GenePrint 10 System PCR Amplification kit (Promega Corporation) was used to determine the genetic signature of both samples based on the multiplex analysis of 9 loci and the Amelogenin sex-determining marker ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the GoTaq 1-step RT-qPCR system (Promega). Knockdowns were verified using primers described in Table I ...
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bioRxiv - Bioengineering 2021Quote: PCR products containing the trigger T7 transcriptional unit were cleaned by PCR cleanup kit (Promega, A6754) and 250 ng were processed by in vitro transcription using the HiScribe T7 kit (Promega ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System kit (Promega) and ligated into pET17b vector using the Gibson Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... Primer concentrations for RT-qPCR reactions were initially evaluated by performing 25μL PCR reactions using Go Taq® Master Mix (Promega Corporation) with approximately 100μg of gDNA ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were performed using 20 ng of cDNA of WT2 and NBS8 organoids using GoTaq® DNA Polymerase (Promega). Total RNA from commercially human fetal and adult brain (BioChain® ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qPCR) reactions were conducted in a 10-µL total volume using a 2× GoTaq® qPCR Master Mix (Promega) and run on an ABI 7500 qPCR thermocycler (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... Reverse transcription (RT) was performed with the GoScript™ Reverse Transcriptase kit Random Primers (Promega, A2801) to convert 1 μg of RNA into cDNA ...
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bioRxiv - Bioengineering 2020Quote: ... amplicons from three PCR runs for each root sample were combined (Clement et al., 1998) and then purified using a PCR purification kit (PCR Clean-up Kit; PROMEGA Inc., Wisconsin, USA).
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL GoScript™ RT Mix for 1-Step RT-qPCR (Promega, Madison, WI), 0.75 μL primer/probe sets for either N1 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... (vi) BP4_DM2_H-(GoScript RT, Promega) consisted of 9 DM2 and 4 control samples ...
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bioRxiv - Molecular Biology 2019Quote: ... (v) BP3_DM1_H-(GoScript RT, Promega) consisted of 2 DM1 and 4 control samples ...
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bioRxiv - Microbiology 2020Quote: ... The dcas9 PCR amplicon was purified using a Wizard SV Gel and PCR Clean-up kit (Promega) and digested with AscI and HindIII ...