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Citations for Promega :
451 - 500 of 4937 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR cleanup was conducted using the Wizard SV Gel and PCR Clean-Up System (Promega) with elution in 30 μl of double distilled water ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) in line with the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and gene expression levels measured using a 1-step RT-qPCR master mix (Promega, UK) and a 7500 Fast qPCR instrument (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2021Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized from nuclear and cytoplasmic RNA using Improm II RT (Promega, Cat. #A3800), and cDNA levels were measured by qPCR using the Brilliant III Ultra-Fast qPCR Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: Extracted vRNA from faecal and tissue samples were reverse transcribed using M-MLV RT (Promega) and random hexamer primers (Roche) ...
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bioRxiv - Microbiology 2023Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Molecular Biology 2023Quote: ... SYBR Green-based RT-qPCR reactions were set up using GoTaq qPCR Master Mix (Promega) and 20 ng of input cDNA per each 10-μL reaction ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Biochemistry 2024Quote: ... plates were placed for 15 min at RT to equilibrate before NanoBRET NanoGlo Substrate (Promega) was added ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Evolutionary Biology 2019Quote: ... Raw PCR products were then used to generate digoxigenin-labeled RNA probes using a T7 RNA in vitro transcription kit (Promega / Life Technologies). RNA was ethanol precipitated and resuspended in water to analyze on a Nanodrop ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Genomics 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA for PCR was isolated from 50 µl of tissue lysate using the Maxwell RSC tissue DNA Kit (Promega, Madison, WI, USA).
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bioRxiv - Plant Biology 2022Quote: ... The two PCR-amplicons were fused by overlap extension PCR and cloned into pGEM-T (Promega) by TA cloning ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were concentrated and gel purified using Wizard SV Gel and PCR cleanup protocol (Promega). Binding reactions consisted of the manufacturer’s binding buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR products were gel-purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and cloned into the pGEM-T Easy Vector (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using a Wizard® SV Gel and PCR Clean-up System (Promega). Samples were barcoded using Oxford Nanopore PCR barcodes (EXP-PBC001 ...
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bioRxiv - Immunology 2022Quote: ... The PCR product was then purified using the Wizard SV 96 PCR Clean-Up System (Promega) and barcoded in a second PCR using two-sided six-nucleotide barcoded primers to discriminate between TCRs of different T cell populations ...
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bioRxiv - Zoology 2020Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean–Up System (Promega, USA) and sent for sequencing to Macrogen Inc ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were purified (Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI)) and sequenced at the UC Davis DNA Sequencing Facility http://dnaseq.ucdavis.edu/.The DNA sequences were compared against the National Center for Biotechnology Information (NCBI ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega). Purified genomic DNA and RT PCR products (100 ng ...
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bioRxiv - Plant Biology 2021Quote: ... PCR reaction mixture was formulated using 5 μl GoTaq G2 green PCR master-mix (Promega, USA), 1 μl primer (forward and revers ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragment was gel-purified using Wizard SV gel and PCR Clean-up System (Promega), ligated into pGEM-T (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of exon 2 of the NR2F2 gene was performed using PCR Master Mix (Promega; primer pair is listed in Appendix Table S1) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Wizard® SV Gel and PCR Clean-Up system (Promega) and then sequenced by Sanger sequencing at GENEWIZ (South Plainfield ...
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bioRxiv - Microbiology 2021Quote: ... The PCR master mix (Promega, USA) was used as per the manufacturer instructions to amplify the 16s rRNA marker gene ...
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bioRxiv - Microbiology 2022Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of nsp4 RNA using mMESSAGE mMACHINE T7 Transcription Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard PCR conditions for GoTaq (Promega) were used as follows ...
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bioRxiv - Plant Biology 2019Quote: ... GoTaq PCR Mastermix (Promega, Mannheim, Germany) was used to monitor cDNA amplification ...
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bioRxiv - Developmental Biology 2020Quote: ... 64 µM PCR Nucleotide Mix (Promega), 1.5 mM MgCl2 and 0.2 µM of upstream and downstream primer each ...
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bioRxiv - Microbiology 2020Quote: ... PCR product was column purified (Promega) for subsequent in vitro transcription of N RNA using mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... PCRs were performed using GoTaq (Promega) on a Veriti thermal cycler (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed with GoTaq (Promega) and with primers listed in Table S9.
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bioRxiv - Microbiology 2023Quote: ... [26]) and PCR chemicals by PROMEGA© with the following PCR conditions ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR nucleotide mix (Promega, U144B). Quantitative PCRs were performed in triplicate using primers against BNIP3 (Pair 1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... TaqMan universal PCR master mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (BioRad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR nucleotide mix (Promega, #U144B) were used to synthesise cDNA from 1 mg of RNA ...
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bioRxiv - Microbiology 2023Quote: ... 0.2 mM PCR Nucleotide Mix (Promega) 10 μM of each primer ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Developmental Biology 2020Quote: ... All RACE-PCR products were purified using GeneJET Gel Purification Kit (Fermentas, Hanover, MD, USA) and ligated into pGEM-T vector (Promega, Madison, WI, USA) and transformed into competent JM-109 Escherichia coli cells (Promega ...