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Citations for Promega :
351 - 400 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... First strand cDNA synthesis was carried out using 2 ug of total RNA and reverse transcription was performed using an oligo (dT) 15 primer and Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA). qPCR was carried out using SYBR GREEN PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... oligo deoxythymidylic acid (oligo-dT) primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega, Madison, WI, USA). RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... PHH were infected with HepAD38-derived GTD virus at 500 viral genome equivalents per cell in maintenance medium supplemented with 4 % PEG 8000 (Promega, Madison, WI; V3011). For patient sera infections ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Genomics 2020Quote: ... Five micrograms of RNA was reverse transcribed into cDNA using Moloney-murine leukemia virus reverse transcriptase and random primers (Promega, Madison, Wisconsin, USA) in a 20 μl reaction volume ...
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bioRxiv - Plant Biology 2022Quote: ... Subsequently 2 μg of RNA were used to perform reverse transcription in the presence of Moloney murine leukemia virus reverse transcriptase (Promega, Madison, WI, USA) after annealing with an anchored oligo(dT)18 primer as described by (Wirth et al. ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... 400 µL PCR products were purified by Promega Wizard Gel/PCR purification columns ...
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bioRxiv - Neuroscience 2021Quote: ... purified with Wizard Genomic DNA Purification kit (Promega) and confirmed via sequencing (GeneWiz) ...
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bioRxiv - Bioengineering 2021Quote: ... and purified using the PureYield MidiPrep system (Promega).
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bioRxiv - Biophysics 2022Quote: ... and subsequently purified by gel purification kit (Promega).
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following the standard protocols ...
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bioRxiv - Biochemistry 2023Quote: ... purified PsVs were treated with DNase I (PROMEGA) to remove unencapsidated DNA associated with capsids ...
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bioRxiv - Microbiology 2022Quote: 0.5mg of plasmid DNA (purified pAdvantage plasmid, Promega), 1mg of bacterial genomic DNA (isolated from E ...
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bioRxiv - Neuroscience 2023Quote: ... Purified HaloTag protein (#G4491) was purchased from Promega. Pluronic F-127 (# P3000MP) ...
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bioRxiv - Microbiology 2020Quote: ... NanoLuc luciferase activity in the lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and a Modulus II Microplate Multimode reader (Turner BioSystem ...
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bioRxiv - Immunology 2021Quote: ... and NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... was in vitro transcribed and translated with a reticulocyte lysate system TnT T7 (Promega, Fitchburg, WI) by incubation of plasmid DNA (1 mg ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were lysed and bait proteins in the lysates were bound to HaloLink Magnetic Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Gaussia luciferase activity was then measured from 10ul of lysate using the Renilla luciferase kit (Promega) with a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: ... and Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and a Glomax Navigator luminometer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and luciferase activity was measured in cell lysates (Renilla luciferase assay system, Promega Madison WI, USA).
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase assays were performed on 20 μL of cell lysates using Luciferase Assay System (Promega) according to the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: ... free and total cholesterol concentration in lysates was determined using Cholesterol/Cholesterol Ester-Glo Assay (Promega) following manufacturer recommendations ...
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Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... the cell lysate was pelleted and read for RLU using a luminometer (Promega GloMax 20/20).
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bioRxiv - Molecular Biology 2022Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Molecular Biology 2020Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega, USA). Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... the luciferase activity in the cell lysates was determined using the Dual Luciferase Reporter System (Promega). Firefly luciferase activities were normalized to that of Renilla luciferase ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were assessed using the Dual-Luciferase Reporter Assay System (#E1910; Promega, Madison, WI, USA). Luminescence was measured using a TECAN M200 infinite-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... Luminescence activity in the resulting cell lysates was measured using the Luciferase Assay System (Promega, USA).
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bioRxiv - Molecular Biology 2019Quote: ... and lysates were assayed for dual luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Genomics 2020Quote: DNA extraction was performed on the phage lysate using the Wizard DNA Clean-Up kit (Promega) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Immunology 2020Quote: ... and NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Immunology 2021Quote: ... Luciferase activity in total cell lysates was measured using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Cell Biology 2022Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) used the SP64 vector as a backbone (Promega). For in vitro insertion reactions ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were used to measure Luciferase activities with the Dual-Luciferase Reporter Assay System (Promega) as indicated by the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity of lysates was then measured using the Nano-Glo Luciferase Assay System (Promega) with GloMax Discover System reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...