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Citations for Promega :
551 - 600 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... the cells were was lysed and Cell lysates were subjected to dual reporter luciferase assays according to the manufacturer’s instructions (Promega).
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bioRxiv - Microbiology 2024Quote: ... Luciferase activity in the cell lysates was determined using the Dual-Glo luciferase assay system (Promega, Madison, WI, USA). The degree of cell entry was directly proportional to the Renilla luciferase units and normalized to firefly luciferase activity.
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bioRxiv - Cell Biology 2024Quote: ... Aliquots of the cell lysates were used to read luciferase emission using Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were harvested and luciferase activity of cell lysates was measured using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Cell Biology 2024Quote: ... Stag2 and Pds5b variants were first expressed by ‘cold’ coupled in vitro transcription- translation (IVTT; TNT reticulocyte lysate, Promega). IVTT aliquots (3 µl ...
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bioRxiv - Immunology 2024Quote: ... cellular lysate was assayed for luciferase and Renilla activities using the Dual-Luciferase Reporter assay system (Promega Madison WI). Luminescence was measured using Glomax 20/20 luminometer (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Evolutionary Biology 2021Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we implemented a cell-free assay with purified recombinant luciferase (Promega) and luciferin (BrightGlo Luciferase Assay System ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Plasmids were purified using PureYield miniprep kits (Promega, Madison, WI, USA), and coding sequences were verified by complete sequencing ...
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bioRxiv - Cell Biology 2021Quote: ... In vitro kinase reactions using purified CDK1/CyclinA2 kinase (Promega V2961) were carried out in a 25 μl reaction containing 100 ng of 6His-MSH-5 ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Systems Biology 2021Quote: ... purified using Wizard SV Gel and PCR Clean-Up System (Promega), and all 4 reactions were pooled together ...
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bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
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bioRxiv - Immunology 2022Quote: Total RNA was purified using ReliaPrep RNA Miniprep System (Promega, #Z6011), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... purified RNA was reverse transcribed using the Reverse Transcription System (Promega) alongside parallel reactions without reverse transcriptase to exclude gDNA contamination ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GloSensor cAMP reagent and purified LgBiT NLuc fragments were from Promega Corporation (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... Halo-tag containing proteins were purified using Magnetic HaloLink Resin (Promega) at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Purified and predigested yeast standard was purchased from Promega (Madison, USA) and resuspended in 0.1% formic acid (FA) ...
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bioRxiv - Developmental Biology 2021Quote: The purified RNA was treated with RQ1 RNase-Free DNase (Promega). Reverse transcription was performed from 1 μg of total RNA using SuperScript™ II Reverse Transcriptase (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was then digested into peptides using Trypsin (Promega, V5111) and desalted using Pierce C18 Spin Columns (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... we purified the digested products using a spin column (A9281, Promega) to avoid organic solvent contamination ...
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bioRxiv - Developmental Biology 2020Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... purified genomic DNA (with 1% unmethylated lambda DNA spike-in, Promega) was sheared to a fragment size of 100 - 700 bp (primary size 250 bp) ...
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bioRxiv - Genomics 2020Quote: ... The cDNAs were subsequently purified with ProNex magnetic beads (Promega, WI) following the instructions in the Iso-Seq Express Template Preparation for Sequel and Sequel II Systems protocol (Pacific Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were purified using a Wizard DNA Clean-Up Kit (Promega) according to manufacturer’s standards.
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bioRxiv - Evolutionary Biology 2021Quote: ... gel-purified (Wizard SV Gel and PCR Clean-Up System, Promega) and blunted using PfuUltra HF DNA polymerase (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was purified with a purification kit (Promega, USA), and then sequenced by the dideoxy termination reaction using an AB3130 DNAxl sequencer ...
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bioRxiv - Immunology 2020Quote: ... and further purified using the ProNex size-selective purification system (Promega) to select products between 500-700 bp range ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were purified using PCR purification kit (Promega, WI, US), and ligated into PCR II Topo-vector (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was purified using the ReliaPrep RNA Miniprep System (Promega) or TRIzol Reagent (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Amplified cDNA samples were purified with ProNex beads (Promega, WI, USA) using the standard workflow and assuming that most transcripts are ∼2 kb ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments were purified using Wizard SV Plus Cleanup Kit (Promega), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Purified RNA was treated with RQ1 RNAse-free DNAse (Promega, M6101) using 1 μL DNase per 5 μL RNA in a final volume of 10 μL volume ...
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bioRxiv - Bioengineering 2024Quote: ... Quenchbody PCR products were purified using Wizard Clean up kits (Promega; as per the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified using the Wizard Genomic DNA Purification kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the purified protein was incubated with 50 µM Halo-Alexa488 (Promega) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Purified EDIII antigens were biotinylated using HaloTag PEG biotin ligand (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Samples were further purified using Wizard Genomic DNA Purification Kit (Promega) following the protocol for Isolation of Genomic DNA from Gram Positive and Gram-Negative Bacteria starting at step 3 ...
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bioRxiv - Immunology 2024Quote: Recombinant antibodies were purified using protein G magnetic beads (Promega #G7472), HiTrap protein G HP columns (Cytiva #17040401) ...
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bioRxiv - Microbiology 2024Quote: Viral genomes were purified using Wizard genomic DNA purification kit (PROMEGA). To determine the amplification kinetic ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µL lysates were loaded into a 96 well plate and luminescence following administration of 50 µL Luciferase Assay Reagent II (Promega) and 50 µL Stop & Glo Reagent (Promega ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... After 24 h luciferase activity was determined from at least four independent experiments with 20 μl of cleared lysate in an LB 9501 luminometer (Berthold) by using the luciferase assay system from Promega.
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reporter gene expression was assessed in 20 µl of cell lysates using the Dual Luciferase Assay and a GloMax luminometer (Promega). To control for transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...