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Citations for Promega :
501 - 550 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with ProNex size-purification system (NG2001, Promega) with 8:5 beads to sample ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA was purified using RQ1 RNase-free DNase (Promega) then cDNA generated using M-MLV reverse transcriptase and random primers (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with Anti-Beta-Galactosidase Purified Monoclonal Antibody (Promega), 1:200 in blocking solution for 2 h ...
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bioRxiv - Bioengineering 2024Quote: ... and purified using the PureYield MidiPrep System (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed 24 hours after transfection and the lysates were subjected to dual-luciferase reporter (DLR) assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2022Quote: ... Firefly and Renilla luciferase activity of cell lysates were sequentially assayed using the Dual-Luciferase Reporter Assay System (Promega) on a GloMax Explorer luminometer (Promega) ...
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bioRxiv - Microbiology 2019Quote: ... RLUs were determined by luciferase assay of cell lysates according to the manufacturer’s protocol (SteadyGlo Luciferase Assay System, Promega) and measured in a Victor Light 1420 plate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2019Quote: ... the Luciferase activities of whole cell lysate were measured using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) by a GloMax20/20 Luminometer (Promega) ...
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bioRxiv - Cell Biology 2019Quote: Target proteins were in vitro transcribed and translated in rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with 35S-Methionine (IsoLabel L-35S Steady Blue ...
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bioRxiv - Microbiology 2021Quote: ... 5 μL of DNA or 5 μL of cell lysate were mixed with 12.5 μL of GoTaq Colorless Master Mix (Promega), 1 μL of forward and reverse primer (5 μM ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Microbiology 2021Quote: IL-18 truncations were generated through in vitro transcription/translation using pET-pro-IL-18 as a template in 10 µl reaction volumes following the manufacturer’s recommendations (TNT Coupled Reticulocyte Lysate; Promega) as previously [40] ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from high titer lysates using the Wizard® Clean-Up Kit (cat. # A7280, Promega, WI, USA). Sequencing libraries were constructed with the NEBNext® UltraTM II DNA Library Prep kit (New England Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were harvested by washing once with PBS wash followed by addition of 22µl of Passive Lysis Buffer (Promega) under shaking for 15min ...
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bioRxiv - Molecular Biology 2020Quote: MC Stem-HalV and GUS-(HalV IGR) mRNAs (3 pmol) were translated in 20μl reaction volume of Flexi rabbit reticulocyte lysate (Promega) in the presence of [35S]methionine (>37.0 TBq/mmol ...
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bioRxiv - Microbiology 2020Quote: ... In vitro transcription/translation was performed with the corresponding primers in Table 1 using pET-pro-IL-1β as a template and following the manufacturer’s recommendations in 10 µl reaction volumes (TNT Coupled Reticulocyte Lysate; Promega). Loading for IL-1R reporter assays was normalized by total IL-1β product measured by enzyme-linked immunosorbent assay (R&D Systems).
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bioRxiv - Cell Biology 2021Quote: ... The Firefly luciferase and Renilla luciferase levels in the lysates were measured using Dual Luciferase Assay (Promega, Madison, USA). For overexpression by adenoviral mediated delivery ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of tissue or cell lysates were mixed with 50µl of Bright-Glo™ Luciferase Assay System (Promega) in a 96-well luminometer plate ...
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bioRxiv - Cell Biology 2022Quote: Total amounts of free cholesterol and cholesterol esters were determined in mouse islet lysates as per the manufacturer’s instructions (Promega). Phosphatidylserine levels were measured in mouse islet lysates as per the manufacturer’s instructions (LSBio).
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bioRxiv - Microbiology 2021Quote: ... and Tat-dependent increase of luciferase enzyme activity in cell lysates was determined with the Luciferase Assay system (Promega). Luminometric activity was analyzed with a Mithras luminometer.
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bioRxiv - Genomics 2022Quote: ... 2 µl of yeast lysate was used as a template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μL of cell lysate from each sample to 50 μL of the GSH-Glo™ Reagent 2X (Promega) with DTT (~2.5 mM final ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg lysate was mixed with 0.1 µl RNase A (~3 mg/ml) and increasing concentrations of RNasin (Promega), as indicated ...
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bioRxiv - Plant Biology 2020Quote: ... We found that using rabbit reticulocyte (TnT® T7 Coupled Reticulocyte Lysate System, Recombinant RNasin® Ribonuclease Inhibitor- Promega) instead of wheat germ extract for in vitro transcription and translation of the ABFs significantly reduces the background noise and increased the quality of peaks that are able to be pulled down from DAP-Seq ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with 50µL of luciferase assay reagent (Luciferase assay system, Promega). We immediately measured the luciferase activity with a Tecan SPARK multifunctional microplate reader (Tecan ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was determined in 30 μL parasite lysates by adding 30 μL luciferin substrate (Promega Luciferase Assay System) bioluminescence detected with a 10 s integration constant (GloMax®-Multi+ Detection System).4 MB and MMV39004853 were used as positive controls and IC50 determined with non-linear curve fitting (GraphPad Prism 6 ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in cell lysates were measured by using either Firefly or Renilla luciferase kits (Promega, Madison WI, USA), and light emission was measured in arbitrary light units on a Glomax 20/20 Luminometer (Promega ...
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bioRxiv - Cell Biology 2020Quote: Firefly and Renilla luciferases were assayed from whole cell lysates with the Dual-Luciferase® Reporter Assay System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and the lysate was used in dual luciferase assays using the Dual-Glo luciferase system (Promega), and measured on the GloMax Discover plate reader (Promega).
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bioRxiv - Microbiology 2020Quote: ... and lysates used to determine firefly and renilla luciferase activities on a Glomax luminometer using luciferase assay system (Promega) and coeleterazine (Promega) ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
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bioRxiv - Molecular Biology 2022Quote: ... The mRNA at a concentration of 214 ng/µL was translated in the Flexi Rabbit Reticulocyte Lysate System (Promega) for 25 minutes at 32°C resulting in run-off ribosome nascent chain complex (RNC) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were diluted 10-fold with 25 mM ammonium bicarbonate and digested with 3 µg of trypsin (Promega, v5111) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Physiology 2023Quote: ... neutralized with 1M Tris solution and lysates were further processed for measurement using the Glucose-Glo Assay (Promega, GmbH) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2023Quote: ... NGS of nanobiopsied mitochondria was compared to lysate obtained from single-cells collected by LCM (29,50) and control genomic DNA (gDNA) (Promega), sequenced using conventional sequencing methods (13).
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bioRxiv - Genomics 2023Quote: ... 2 µL of yeast lysate was used as a template in a 10 µL GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: ... Firefly and Renilla Luciferase activities in the lysate were then determined with a Dual-Luciferase Reporter Assay System (Promega) and a GloMax explorer plate reader (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... 20µL of the lysate was revealed using the Dual-Glo luciferase assay system following the manufacturer’s protocol (Promega, E1910). Luminescence was measured using a FLUOstar Optima plate reader (BMG).
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bioRxiv - Microbiology 2023Quote: ... and the light units (LU) per µl of lysates were measured using Nano-Glo® Luciferase Assay System (Promega) in a luminometer (BioTek Synergy LX Multimode Reader) ...
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bioRxiv - Microbiology 2023Quote: ... and the luciferase activity of the cell lysates was analyzed with the dual-luciferase reporter assay system (Promega, E1960) using a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and luciferase activity of cell lysates was measured using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Immunology 2024Quote: ... cellular lysate was assayed for luciferase and Renilla activities using the Dual-Luciferase Reporter assay system (Promega Madison WI). Luminescence was measured using Glomax 20/20 luminometer (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... cell lysates were harvested and luciferase activity was measured using the Dual-Luciferase® Reporter Assay System (Promega, USA) according to the manufacturer’s instructions ...