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Citations for Promega :
201 - 250 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... reverse transcribed with oligo(dT)15 in a 20-μL reaction mixture using the Moloney murine leukemia virus reverse transcriptase (Promega) or the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of culture supernatants were collected every 6 hours and nanoluciferase activities associated with released virus in the supernatants were measured using Nano-Glo luciferase assay system (Promega).
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated for three days before assessing viability and virus-induced cell death by CellTiter Glo according to manufacturer protocol (Promega). Luminescence was quantified using a plate reader (Cytation 5 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was used for first-strand cDNA synthesis using Moloney murine leukemia virus (MMLV) reverse transcriptase enzyme (Promega, USA) and random primers (Biodynamics ...
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bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of each RNA sample was reverse-transcribed using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT, Promega, #M1701) and random hexamer primers (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... Clear lysates were treated with 5U RQ1 DNase and 5U RNase inhibitor (Promega) at 37°C for 15 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... The lysates were sequentially treated with 12.6 μl of RNase-free DNase (Promega) and 7.5 μl of 1:1330 diluted micrococcal nuclease (11.3 gel units ...
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bioRxiv - Microbiology 2020Quote: ... 15µl of lysate were mixed with 50µl of Nano-Glo assay buffer (Promega) in a flat bottom white-walled luminescence plate ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were analyzed for luciferase activity using GloMax Discover Microplate Reader (Promega). For wild-type SARS-CoV-2 infection ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were collected and analysed using the dual-luciferase assay kit (Promega) following the manufacter’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates were collected 24 hours post-transfection using Passive lysis buffer (Promega). Samples from three technical replicate transfections were measured in duplicate using a Zylux FB12 luminometer and the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... Epe1 fragments were translated in vitro using TNT T7-coupled reticulocyte lysate (Promega) with 35S-labeled methionine (Roche) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were analysed using the Dual-Luciferase Reporter Assay System (Promega, #E1910). Luminiscence was measured using a TECAN infinite M200-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysates were subjected to luciferase activity testing (Promega, Madison, WI, USA) 24 h later ...
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bioRxiv - Immunology 2021Quote: PP2A activity in cell lysates was measured using the Phosphatase Assay Kit (Promega). Endogenous free phosphate was removed using the columns ...
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bioRxiv - Developmental Biology 2021Quote: ... lysates were simultaneously analyzed for firefly luciferase activity and Renilla luciferase activity (Promega).
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bioRxiv - Neuroscience 2021Quote: ... Lysate samples are generated by treatment of 1x Passive Lysis Buffer (Promega E1941) to 12 well plate cultures ...
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bioRxiv - Microbiology 2022Quote: ... Seventy five ug of each lysate were digested with sequencing-grade trypsin (Promega) 1:100 ratio for 12 hours at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were used for luciferase assays (20 µL) with Luciferase assay system (Promega) and RNA extraction (20 µL ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation assays were performed using nuclease-treated rabbit reticulocyte lysate (Promega). Reactions were in 10 µl containing 2 µl RRL ...
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bioRxiv - Immunology 2023Quote: ... cell lysates were analyzed using the Dual-Luciferase® Reporter Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The luminescence of lysates was detected by Luciferase Assay System (Promega, Cat# E1500) and analyzed using a plate spectrophotometer ...
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bioRxiv - Biophysics 2023Quote: ... Luciferase activity of lysates was measured using the Dual Luciferase Assay Reagents (Promega) on a GloMax Multi microplate luminometer (Promega) ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in cell lysates collected 8 hours after transfection (Promega). To correct for transfection efficiency ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). Capped and polyadenylated reporter mRNAs containing the first 135 nucleotides of NPR coding sequences (human ...
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bioRxiv - Molecular Biology 2024Quote: Rluc mRNA and Fluc mRNA were incubated with rabbit reticulocyte lysate (RRL, Promega) to generate the mRNA-ribosome complex ...
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bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Immunology 2021Quote: ... to each cell lysate and measuring the luminescence using a GloMax portable luminometer (Promega).
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer, Promega) was used to correct for transfection efficiency.
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bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase expression in the cell lysates was determined using a dual-luciferase kit (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Pathology 2021Quote: ... Fluorescence of the lysates was then analyzed using a multi detection plate reader (Promega Glomax Multi Detection System ...