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Citations for Promega :
701 - 730 of 730 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 10 μL of lysate was mixed with the appropriate luciferase assay substrate and light emission was measured by using a Glomax 20/20 Luminometer (Promega, Madison, USA).
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bioRxiv - Microbiology 2020Quote: ... 10 ul of the cell extract was mixed with the appropriate luciferase assay substrate and light emission was measured by using a Glomax 20/20 Luminometer (Promega, Madison, USA).
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2021Quote: ... the samples were incubated for 5 min at room temperature with equivalent volumes of Bright-GloTM firefly luciferase substrate (Promega, Madison, WI), and the luciferase signal was measured using a TopCount NXT plate reader (PerkinElmer Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... LU were measured on a Berthold LB 960 Centro luminometer (Berthold Technologies, Oak Ridge, TN) using a coelenterazine substrate mixture (Promega, Madison, WI). All samples were run in duplicate.
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed in 100 μL of passive lysis buffer and 50 μL lysate was incubated with 100 μL of luciferase assay substrate according to the manufacturer’s instructions (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 48h post-transduction the luciferase activity was read by adding luciferase substrate (ONE-Glo EX luciferase assay system, Promega, Wisconsin, USA) using a luminescence plate reader (Envision ...
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bioRxiv - Molecular Biology 2021Quote: ... a standard 12.5 μl translation reaction was mixed with 50 μl 1x Renilla-Glo substrate in Renilla-Glo assay buffer (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Bioengineering 2023Quote: Mice were administered either 200μL of 15 μg/mL D-luciferin or a 1:40 dilution of Nano-Glo substrate (Promega, diluted in DPBS) by intraperitoneal injection for firefly luciferase and Antares or nanoluciferase imaging ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl of supernatant was removed from all wells and replaced with 100 μl of Bright-Glo luciferase substrate reagent (Promega; catalog #E2610). After 2 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Dual-luciferase assays were performed using 40 μl of the substrate in the appropriate buffer for a 10 μl in vitro translation assay (Promega, Cat. No. E1960). Luminescence was measured using TECAN Infinite M1000 and plotted on GraphPad (Version 8.4.2).
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bioRxiv - Bioengineering 2019Quote: CYP3A4 and CYP2C9 enzyme activities were measured by incubating the cultures with luciferin-IPA or luciferin-H substrates (Promega Life Sciences, Madison, WI) for 3 hours ...
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bioRxiv - Neuroscience 2021Quote: ... plates were removed from the incubator after 70 hours and 10 µL of 4x RT-Glo substrate and enzyme per well (Promega, Madison, WI, USA) diluted in P/S free medium ...
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bioRxiv - Pathology 2021Quote: ... benthamiana leaves were collected at 48 h after infiltration and then incubated with 1 mM luciferin substrate (catalog no. N1110; Promega Biotech, Beijing, China). LUC images were captured with a Tanon-5200 Multi Chemiluminescent Imaging System (Tanon ...
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bioRxiv - Microbiology 2022Quote: ... Episomal expression of the nanoluc reporter gene was confirmed in luminescence assays using NanoGlo substrate according to instructions of the supplier (Promega, Leiden, the Netherlands). Subsequently ...
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bioRxiv - Molecular Biology 2020Quote: ... and digested with trypsin at an enzyme-to-substrate ratio of 1:50 (wt/wt, modified sequencing grade, Promega, Madison, Wisconsin. United States) for 16 hours at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... Nanoluciferase-based bioluminescence resonance energy transfer (BRET) was measured using NanoBRET™ Nano-Glo® Substrate on a GloMax® Discover Multimode Microplate Reader (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... or stimulated for 10 minutes or 1 hour by BRET through activation of NanoLuc’s bioluminescence with its substrate furimazine (1:100 dilution of Promega nano-Glo Live Assay). After treatment ...
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bioRxiv - Cell Biology 2023Quote: ... a master mix was prepared by diluting the Extracellular NanoLuc Inhibitor at a 1:1000 ratio and the NanoBRET Nano-Glo Substrate at a 1:333 ratio in PBS (Promega, Madison, WI, USA). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was rinsed 10 min three times in PBS 1X-Tween buffer before ECL (Western Blotting Substrate, # W1001 Promega, Madison, WI, USA) revelation by image acquisition with the Fusion Fx chemiluminescence imaging system (Vilber Lourmat ...
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bioRxiv - Immunology 2023Quote: ... prepared by combining ONE-Glo™ EX Luciferase Assay Buffer with ONE-Glo™ EX Luciferase Assay Substrate in 1:1 ratio (Promega, USA). After measuring the signal of the Firefly luciferase in the GloMax® 20/20 Luminometer (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Developmental Biology 2020Quote: ... Digests were carried out in 4M urea 100mM HEPES with LysC (Wako, #121-05063, 1/100 (w/w) protease/substrates) for 3 h at 37C and subsequent trypsin digest (Promega, #V5280, 1/100 (w/w) protease/substrates ...
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bioRxiv - Cell Biology 2021Quote: ... PBS was aspirated and replaced with CO2 independent medium plus live cell substrate according to manufacturer instructions (Promega Nano-Glo Live Cell Assay System, N2013). The cells were incubated for 5 minutes and immediately loaded onto the ClarioSTAR plate reader where luminescent signal was quantified at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were resuspended at 2×106 cells/ml in culture media containing Nano-Glo® Endurazine Live Cell Substrate™ (Promega Inc, WI, USA # N2571) and DrkBiT (Promega Inc ...
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bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
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bioRxiv - Microbiology 2020Quote: ... Light signals derived from reconstituted HiBiT/LgBiT association were measured using Tristar multiwell luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Mock-infected cells served as controls to determine basal luciferase activity for obtaining normalized relative light units in infected samples ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated at 37 °C in tissue culture incubator for 4 hours or 24 hours before measuring reconstituted HiBiT/LgBiT activity using a Tristar multiwell Luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Individual unmixed populations of cells were treated identically and served as controls to determine basal luciferase activity to obtain normalized relative light units ...