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Citations for Promega :
551 - 600 of 730 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... the culture medium was removed and 50 µL of 100x diluted NanoLuc substrate (Furimazine, Promega Nano-Glo, N1120) was added to each well of a 96-well microplate containing the transfected cells ...
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bioRxiv - Microbiology 2023Quote: ... 50 μL of diluted supernatant was then added to 50 μL LDH substrate mix as described by Promega. LDH activity was recorded using the VICTOR3 multilabel plate reader (Perkin-Elmer).
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bioRxiv - Biophysics 2023Quote: ... equal volumes of HiBiT-N VLPs and hACE2-LgBiT EVs were mixed with nanoluc substrate (cat#N2420, Promega) and trypsin (Sigma ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Microbiology 2024Quote: ... Media was removed from each well prior to addition of 100 μL Bright-Glo™ luciferase substrate (Promega) diluted 1:20 in 1x lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... CYP3A4 and CYP2C9 activities were measured by incubating cultures with luciferin-IPA or luciferin-H substrates (Promega, Madison, WI) for 1 or 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The splicing substrate XBP1u mRNA was prepared using a RiboMAX™ Large Scale RNA Production Systems—T7 kit (Promega) at 37°C for 3 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mM Tris–HCl [pH 7.5]) before AP activity was measured using the stabilized substrate for AP (Western Blue; Promega), as described previously [6].
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bioRxiv - Molecular Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white microplate ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...
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bioRxiv - Systems Biology 2019Quote: ... Luminescence was measured by auto-injecting 50 μL Renilla luciferase substrate (Renilla Luciferase Assay system, catalog No. E2820, Promega) per well and integrating light output for 4 seconds using a TriStar luminometer (Berthold).
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bioRxiv - Molecular Biology 2019Quote: ... 20 μl of f/2 and 10X concentrated substrate (10 mM firefly luciferin, 1,000X dilution of NanoLuc Dual-Glo reagent, Promega) were added to the respective wells.
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bioRxiv - Synthetic Biology 2021Quote: ... 10 μL of stopped reactions were added to 50 µL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a 96-well white flat bottom microplate ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were washed three times with TBS buffer containing 0.1% Tween 20 and finally the membranes were developed with BCIP/NBT color development substrate (Promega) to enable visualization of protein bands (Promega ...
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bioRxiv - Bioengineering 2022Quote: ... various concentrations of the compounds were mixed with 1 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: Plates were left to equilibrate at room temperature for 20 minutes before addition of NanoGlo live cell substrate (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 20 mM Tris–HCl [pH 7.5]) before AP activity was measured using the stabilized substrate for AP (Western Blue; Promega), as described previously [9] ...
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bioRxiv - Microbiology 2022Quote: ... and the cells were incubated in the growth medium supplemented with 5µM of cell-permeable Renilla luciferase substrate EnduRen (Promega) at 37C directly in an ID3 multiwell plate reader (Molecular Devices) ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Evolutionary Biology 2022Quote: ... diluted in a luciferase assay buffer (PBS, 0.1% BSA) and mixed with furimazine (0.25% Nano-Glo® Substrate, Promega). The samples were distributed in duplicates in 96 half-well white polystyrene microtiter plates ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was determined in 30 μL parasite lysates by adding 30 μL luciferin substrate (Promega Luciferase Assay System) bioluminescence detected with a 10 s integration constant (GloMax®-Multi+ Detection System).4 MB and MMV39004853 were used as positive controls and IC50 determined with non-linear curve fitting (GraphPad Prism 6 ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Cell Biology 2021Quote: ... Supersignal kit (Pierce) and a LAS-3000 imager (Fujifilm) or AP-coupled antibodies and NBT/BCIP as substrates (Promega). Rabbit polyclonal antibodies obtained and purified using full-length Arpin were previously described (Dang et al ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... NanoBRET™ Nano-Glo® Substrate - furimazine (#N1663) and HaloTag® NanoBRET™ 618 Ligand (#G9801) were from Promega. Dimethylsulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase intensity was then read on a Luminometer with luciferase substrate according to the manufacturer’s instructions (Promega, PR-E2620). 2 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was transferred into a fresh 384-well assay plate containing NanoGlo Luciferase Assay Substrate (Promega, Madison, WI), and luciferase intensity was measured by EnSpire Plate Reader (PerkinElmer ...
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bioRxiv - Microbiology 2022Quote: ... 20 μL of cells were mixed with 20 μL of the CellTiter-Glo 2.0 Cell Viability Reagent or Caspase-Glo 3/7 Assay substrate (Promega). Luminescence was measured after 2 min incubation for CellTiter-Glo 2.0 or 30 min incubation for Caspase-Glo 3/7 Assay using the Synergy H1 microplate reader as above ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Neuroscience 2022Quote: ... Half of the virus containing media was aspirated and replaced with luciferase substrate (Bright-glo luciferase assay system, Promega) and incubated for 5 minutes at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were harvested and the medium was exchanged to CO2 independent medium (Life Sciences) containing Endurazine substrate (PROMEGA) according to the manufacturers protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of assay cells were mixed with 10 μL of NanoGlo Live Cell Substrate (Promega, Madison, Wisconsin, USA) and 30 μL of LB to make up a total of 50 μL reaction mixture in an opaque ...
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bioRxiv - Bioengineering 2023Quote: ... imaging was performed 10 min after intraperitoneal (i.p.) injection with 200 μL of 15 mg/mL luciferin substrate (Promega). The total flux of luminescence was calculated by gating a region of interest (ROI ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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Contribution of circulating host and microbial tryptophan metabolites towards Ah receptor activationbioRxiv - Biochemistry 2023Quote: ... Luciferase activity was measured using a TD-20e luminometer (Turner Biosystems Inc.) and luciferase substrate (Promega, Madison, WI, USA), per the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Lysates (20 mg per well) were assessed in 96-well plates using 90 μl reconstituted Luciferase Assay Substrate (Promega) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2023Quote: Serine/threonine phosphatase activity assay of PP2A was performed using the artificial p-peptides RRA(pT)VA substrate (Promega) in lysis buffer containing 1 mM MnCl2 at 70 °C 26 ...
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bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... samples corresponding to 4.44×105 cell equivalents of the translation reaction were put on ice and mixed with 1x Renilla-Glo substrate (Promega) in Renilla-Glo (Promega ...
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:5000 in 5% milk in TBST were used for detection by the addition of an enhanced chemiluminescence (ECL) substrate (Promega). Western blot quantification was performed using Fiji software (Schindelin et al. ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Immunology 2022Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Data were normalised to a mock-treated control to generate a fold induction ...
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bioRxiv - Developmental Biology 2019Quote: ... The medium was supplemented with 1mM Luciferin (NanoLight Technology, Cat#306A) and 0.5µl of RealTime Glo Cell Viability Assay Substrate (Promega, Cat#G9711). For imaging in pluripotency maintenance conditions ...
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bioRxiv - Plant Biology 2019Quote: ... Samples for luciferase assay were prepared by mixing 25 μL luciferase assay reagent (luciferase assay buffer + 1x final concentration luciferase assay substrate) (Promega) with 5 μL of thawed samples in a 384-well microtiter plate ...
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bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells were then harvested and washed prior to cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...