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Citations for Promega :
501 - 550 of 730 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega, UK). Digestion was carried out overnight at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed and luciferase substrates provided using the Dual-Glo Luciferase Assay System (Promega E2920). Renilla and firefly luciferase activity were measured using the LUMIstar Omega luminometer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... firefly and renilla luciferase substrates were applied using the Dual-Glo Luciferase Assay System (Promega, Mannheim) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was imaged using 500 µl of 1:1000 NanoGlo substrate (Promega, catalogue number: N1120) diluted in 10 mM sodium phosphate buffer pH 7.0.
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bioRxiv - Microbiology 2020Quote: ... cells were washes twice and followed by the addition of 50 μL Nano luciferase substrate (Promega). After 5 minutes of incubation at room temperature ...
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bioRxiv - Cell Biology 2021Quote: Substrate RNAs were synthesized by in vitro transcription using a T7 RNA production system (Promega, P1300) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Cell Biology 2021Quote: ... the transfected cells were lysed and mixed with NanoLuc substrate (Nano-Glo Luciferase Assay System; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 μl of each supernatant was added to 50 μl of luciferase assay substrate (Promega). We used a Synergy Neo HTS multimode microplate reader (BioTek ...
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bioRxiv - Systems Biology 2019Quote: ... Luminescence was measured by auto-injecting 50 μL Renilla luciferase substrate (Renilla Luciferase Assay system, Promega) in each well and integrating light output for 4 s using a TriStar luminometer (Berthold ...
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bioRxiv - Neuroscience 2021Quote: ... Substrates, Coelenterazine native (CTZ, NanoLight technology cat. no. 303) and Furimazine (FMZ, Promega cat. no. N1110) were added using a micro-injector connected to the plate reader (BioTek Synergy HT) ...
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bioRxiv - Biochemistry 2020Quote: ... The reporter activity was determined using the dual luciferase substrate system (E1910; Promega, Madison, WI, USA), and normalized with the Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... the blots were developed with Western Blue® Stabilized Substrate for Alkaline Phosphatase (Promega, Madison, WI) for roughly 15 sec ...
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bioRxiv - Microbiology 2021Quote: ... PBS buffer having 1,000 volume diluted Nluc-specific furimazine substrate (Nano-Glo Luciferase Assay System, Promega) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... then 50 µL of drug buffer with RLuc substrate coelenterazine h (Promega, 5 µM final concentration) was added per well ...
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bioRxiv - Biochemistry 2022Quote: ... by quickly mixing 2 μL of above refolding mixture with 50 μL of luciferase substrate (Promega). The activity of unheated luciferase in buffer B was set as 100%.
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bioRxiv - Cancer Biology 2023Quote: ... then the firefly luciferase substrate (20 μl) was added (Bright-Glo™ Luciferase Assay Kit, Promega). Light unit values were normalized to the OD600 absorbance of each culture that was measured from the 96-well culture plates ...
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bioRxiv - Biochemistry 2023Quote: ... the membrane was visualized using 500 μL of 1:1000 NanoGlo substrate (Promega, catalogue number: N1110).
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bioRxiv - Biophysics 2023Quote: ... Bioluminescence was measured after addition of the substrate in a GloMax® Discover Microplate Reader (Promega). All experiments were performed thrice in triplicates.
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bioRxiv - Biochemistry 2023Quote: ... HRP (4 μM) was added followed by 20 μL of Nano-glo luciferase substrate (Promega, N1110) to each well ...
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bioRxiv - Synthetic Biology 2023Quote: ... then adding 30 μl of pre-mixed nGlo buffer with the recommended substrate concentration (Promega, #N1110). Luminescence was read in black opaque 96 well plates (Thermo Scientific ...
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bioRxiv - Pathology 2024Quote: ... Cell Titer-Glo® Buffer and Substrate were mixed per the manufacturer’s instructions (Promega, Southampton, UK), and then added 100 µl to each well containing the treated cells ...
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bioRxiv - Cell Biology 2024Quote: ... Nano-Glo assay reagent consisted of a 1:1000 dilution of Nano-Glo substrate (Promega; N1110) in buffer (100mM 2-(N-Morpholino)ethanesulfonic acid hydrate (M8250 ...
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bioRxiv - Biochemistry 2024Quote: ... after addition of the diluted NanoLuc substrate (10 μl/well, 30-fold dilution of the Promega substrate stock using the activation solution) ...
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bioRxiv - Bioengineering 2024Quote: ... the Firefly luciferase substrate (LAR II reagent, 100 µL/well, Promega Dual Glo kit, cat # E1960) was added to each well ...
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bioRxiv - Microbiology 2024Quote: ... Products and substrate molecules were analyzed by LC-MS or with NAD/NADH-Glo kit (Promega).
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bioRxiv - Microbiology 2024Quote: ... using 20 uL of each extract into which 20 uL luciferase assay substrate was injected (Promega). β-galactosidase activity was measured by absorbance at 420 nm in the Tecan Genios plate reader using 30 uL of each extract mixed with 30 uL of 2x Assay Buffer (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... the PI3K lipid kinase reaction was performed in the presence of PIP2:3PS lipid substrate (Promega #V1792) and ATP ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with the assay substrate Nano-Glo containing the furimazine at 200-fold dilution (#N1110, Promega, France).The bioluminescence signal of Nluc donor and the YFP acceptor emission were acquired using an EnVision Multimode Plate Reader (Perkin Elmer ...
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bioRxiv - Biochemistry 2019Quote: ... Vmax and kcat values for given kinase substrates were determined using the ADP-Glo Kit system (Promega). In all cases reactions were carried out in 384 well plates at room temperature for 30 min with a total kinase concentration of 50 nM and 1 mM ultra-pure ATP ...
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with trypsin (trypsin to substrate ratio of 1:50, Promega, cat. no. V5111) and 1 mM CaCl2 for 16 h at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activities were measured in 10 μl of lysate using 50 μl luciferase assay substrate (Promega). To normalise transfection efficiencies β-Galactosidase levels were measured using the β-Galactosidase Enzyme Assay System (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were detected using a G:BOX (Syngene) after treating membranes with ECL Western Blotting Substrates (Promega). Densitometry was performed using ImageJ software.
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bioRxiv - Microbiology 2023Quote: ... After the addition of 1x Nano-Glo Lysis Buffer and 1:1000 Nano-Glo substrate (Promega, USA), relative light units (RLU ...
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bioRxiv - Plant Biology 2023Quote: ... The chemiluminescent signal of HRP conjugated to secondary antibodies was detected with ECL Western Blotting Substrate (Promega) using a ChemiDoc XRS+ system (Biorad).
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bioRxiv - Cell Biology 2023Quote: ... the injectors were washed following the manufacturer’s protocol and primed with the luciferase assay substrate (Promega, USA) diluted in water (1:10) ...
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bioRxiv - Bioengineering 2023Quote: The luminescence of each well was measured 24 h later using the Bright-Glo substrate (Promega, E2610) and a Tecan Infinite M200 Pro plate reader.
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bioRxiv - Biochemistry 2023Quote: ... and assayed with 4 μM of HRP and 20 μL of Nano Glo luciferase substrate (Promega, N1110) diluted to 1:1000.
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bioRxiv - Immunology 2023Quote: Proteasome activity was measured in cell lysates using the Proteasome-Glo™ 3 Substrate System (Promega, G8531). Corresponding reagents for testing as chymotrypsin-like ...
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bioRxiv - Systems Biology 2024Quote: ... followed by 80μL Hank’s balanced salt solution (HBSS) containing 10mM HEPES and 10μM furimazine/NanoGlo Luciferase Assay Substrate (Promega). BRET measurements were performed at 37 °C using a PHERAstar Microplate Reader (BMG Labtech ...
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bioRxiv - Synthetic Biology 2021Quote: ... that was mixed with 100 μL of pre-prepared Nano-Glo® Luciferase Assay Buffer + Substrate (Promega # N1130) and incubated for 20 minutes at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... the culture medium was removed and 50 µL of 100x diluted NanoLuc substrate (Furimazine, Promega Nano-Glo, N1120) was added to each well of a 96-well microplate containing the transfected cells ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Cell Biology 2021Quote: ... Perifusate was collected in 96 well plates and analyzed for NanoLuc activity using NanoGlo Luciferase Assay Substrate (Promega) per manual instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was determined by mixing 50 μl of protoplast lysate with 50 μl luciferase substrate (Promega, E1501) in a white 96-well plate and light emission was measured at 1 second/well using a microplate luminometer (Centro ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was mixed with 100 μl luciferase substrate (Bright-Glo Luciferase Assay System, purchased from Promega, USA), and luminescence was measured immediately by the GloMax Navigator System (Promega ...
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bioRxiv - Biophysics 2022Quote: ... HaloTag substrates conjugated to Janelia Fluor® dyes ranging from 525 to 650 nm are available from Promega. Fig ...