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Citations for Promega :
651 - 700 of 730 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... on day three the media was completely removed and 60 µL lysis buffer together with 60 µL luciferase substrate (Promega) was added per well ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Microbiology 2023Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for 10min at room temperature according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: HEK293T cells were transfected with a FLAG-tagged human DHHC20-expressing plasmid and a corresponding HA-tagged substrate-expressing plasmid using FuGENE transfection reagent (Promega) and incubated overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... injecting 50 μl per well of coelenterazine substrate (Nanolight Technologies, 2 μg/ml) and analysing luminescence on a FLUOstar OPTIMA luminometer (Promega). Fold inductions were calculated by normalising to a mock-treated control.
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bioRxiv - Neuroscience 2023Quote: ... 1µl lysate was diluted with 39 µl PBS before being mixed with 40 µl of Dual-Glo FLuc substrate (Promega). After 10 min incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Bioengineering 2023Quote: ... luminescence was read by removing 150 µL of media and adding 50 µL of substrate from the Nano-Glo Luciferase Assay System (N1110, Promega). The mixture was incubated for 3 minutes in a white-walled plate before analysis with a SpectraMax M3 plate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Bioengineering 2024Quote: ... Bioluminescence measurements were taken by combining 25 µL of the sample solution with an equal volume of prepared buffer-reconstituted nluc substrate as detailed by the manufacturer (NanoGlo Luciferase Assay System; Promega). Measurements were taken every 20 min (225 min total ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Immunology 2024Quote: ... Cells were then collected and washed before cell lysis and addition of luciferin substrate from the Bright-Glo Luciferase Assay System (Promega). The resulting luminescent signal was measured using a Tecan Infinite M200 Pro ...
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... The hydrolase activity of the UDP-substrate was detected as the release of UDP by the UDP-Glo assay (Promega) after 20 min of incubation with UDP-Glo detection reagent ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cells were pre-incubated in Hanks’ balanced salt solution with increasing concentrations of compound for 60 minutes before stimulation with 10 nM CXCL12 and addition of 2 µM Renilla Luciferase substrate coelenterazine-h (Promega). After 20 minutes ...
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bioRxiv - Microbiology 2020Quote: ... At 24 h post-transfection media was removed and Nluc enzymatic activity was measured using a Berthold Centro-XS luminometer by injecting 50 μL of luciferase substrate reagent (#E2820, Promega, France) per well and counting luminescence for 2s ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were simultaneously lysed and supplemented with a luciferase substrate by addition of equal volume of Steady-Glo reagent (Promega, E2520) directly to the assay media ...
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bioRxiv - Physiology 2019Quote: ... Samples were mixed with the same amount of substrate provided by the One Glo Luciferase Assay System (Promega, Mannheim, Germany, #E6110) immediately before signal detection ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...
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bioRxiv - Microbiology 2021Quote: ... and nanoluciferase activity was measured using Tristar multiwell Luminometer (Berthold Technology) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate in nanoluc assay buffer (Promega Inc). An uninfected monolayer of Vero E6 cells treated identically served as controls for determining background and obtain normalized relative light units ...
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... per well before adding 50 µL NanoGlo® HiBiT lytic buffer supplemented with substrate and LgBiT according to the manufacturer’s instructions (Promega, N3040). The plates were incubated for 10 min on a shaker in the dark before measuring luminescence on the EnVision 2102 Multilabel reader (Perkin Elmer).
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bioRxiv - Microbiology 2020Quote: ... After 24 hours of growth luciferase activity was visualized by spraying one spray of 1:100 reconstituted NanoGlo Luciferase substrate (Promega N1110) per agar plate using a disposable spray flask ...
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bioRxiv - Immunology 2021Quote: ... Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate in nanoluc assay buffer (Promega Inc, WI, USA). Uninfected monolayer of Vero cells treated identically served as controls to determine basal luciferase activity to obtain normalized relative light units ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with TBS-T and imaged using 500 µl of 1:500 NanoGlo substrate (Promega, catalogue number: N1120) diluted in 10 mM sodium phosphate buffer pH 7.0.
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bioRxiv - Immunology 2020Quote: ... Neutralizing ability disproportionate with luciferase intensity was then read on a Luminometer with luciferase substrate according to the manufacturer’s instructions (Promega, #PR-E2620).
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bioRxiv - Cancer Biology 2022Quote: ... Gaussia princeps luciferase enzymatic activity was measured using a Berthold Centro LB960 luminometer by injecting 50 µl per well of luciferase substrate reagent (Promega, E2820) and counting luminescence for 10 seconds ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Microbiology 2023Quote: ... neutralization was measured as indicator of luciferase activity in the cells (relative luminescence unit) by using nano-Glo luciferase substrate (Promega Inc.). Luminescence was measured by using Cytation-5 multimode reader (BioTech Inc.) ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Microbiology 2023Quote: ... 2 μL of furimazine substrate was added to the reaction well from a working reagent stock of a 2:100 NanoDLR Stop & Glo Substrate to Buffer ratio (Promega #N1610). A total well volume of approximately 112.5 μL was incubated at room temperature for 2 minutes prior to measuring luminescence and OD600 readings on an EnVision plate reader (PerkinElmer).
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bioRxiv - Developmental Biology 2023Quote: ... and 1:2000 RealTime-Glo MT Cell Viability Assay Substrate (to visualise Nluc, first diluted 1:1 in DMSO, Promega G9711).
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bioRxiv - Immunology 2023Quote: ... for 90 min at 37°C (enzyme-to-substrate ratio of 1:100) and subsequently digested overnight at 37°C with trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Microbiology 2023Quote: ... Six and a half hours post-infection cells were lysed in 50 µL of 1X Passive Lysis Buffer and luminescence was determined using the Luciferase Assay Substrate of the Dual-Luciferase® Reporter Assay System (# E1910, Promega), measured on a plate reader (GloMax® Navigator Microplate Luminometer ...
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted 1:100 in fresh LB supplemented with chloramphenicol and kanamycin including a 1:5000 dilution of the Nano-Glo® Luciferase Assay Substrate (Promega). Preliminary growth curves in the presence of the substrate showed no impact on growth at this concentration ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken horizontally at 500 RPM for 5 min before addition of 25 µL of ready-to-use Nano-Glo substrate (Promega; N1130) for measuring luciferase intensity.
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bioRxiv - Synthetic Biology 2023Quote: ... 25 μL of samples was added into white-walled 96-well plates and equal volume of ready-to-use Tluc substrate (Promega; E1501) was injected to each well ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µL of the schizont culture was aliquoted into white 96 well luminometer plates to which 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
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bioRxiv - Microbiology 2024Quote: ... The activity of in vitro synthesized luciferase was assessed using 5 μL of the substrate from the Steady-Glo Luciferase Assay System (Promega, US). The signal was detected on plate reader Victor X5 2030 (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: The activity of the proteasome’s β5 sites was determined either by Succinyl(Suc)-LLVY-AMC (7-amido-4-methylcoumarin) fluorogenic substrate or by the Proteasome-Glo™ assay (Promega), a luciferase coupled assay ...
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bioRxiv - Cancer Biology 2021Quote: ... turnover of ATP to ADP by the phosphorylation of the lipid substrate was detected using the Promega ADP-Glo™ (Promega #V1792) assay ...
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bioRxiv - Neuroscience 2019Quote: ... Gaussia luciferase activity was measured in the dark 0.1 sec after injection of 100 μl/well of 40 mM coelenterazine, substrate (NanoLight, Pinetop, AZ) using a 2 second integration on a Veritas Microplate Luminometer (Promega, Madison, WI). The cells were subsequently assayed for red Firefly activity using the ONE-GloTM Luciferase Assay System kit (Promega ...
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bioRxiv - Immunology 2019Quote: ... generated by the presence of residual intact target cells that have not been lysed by the effector population in the presence of ADCC-mediating mAb (ViviRen substrate, Promega, Madison, WI). The % of specific killing was calculated using the formula ...
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bioRxiv - Immunology 2021Quote: ... generated by the presence of residual intact target cells that have not been lysed by the effector population in the presence of ADCC-mediating mAb (ViviRen substrate, Promega, Madison, WI). The % of specific killing was calculated using the formula ...