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Citations for Promega :
351 - 400 of 764 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: Total mRNA was isolated from tissues using a Maxwell® LEV simply RNA Cells/Tissue kit (cat# AS1280, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: The 3’ UTR of target mRNAs spanning at least 500 bp around the predicted miRNA binding sites were cloned into the psi-CHECK2 vector (Promega, C8021) downstream of the Renilla luciferase gene (Supplementary Table 1) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNAs were produced and purified by in vitro transcription using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega) according to the manufacturer’s protocol and subsequent phenol:chloroform extraction and isopropanol precipitation ...
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bioRxiv - Microbiology 2022Quote: Various KSHV mRNA 5’-UTRs containing uORFs were cloned up stream of the Renilla luciferase gene in a psiCHECK™-2 vector (Promega) using a Gibson Assembly® Cloning Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: Tethering assay in rabbit reticulocyte lysates was performed by applying in vitro transcribed and capped mRNA encoding mApple-YTHDF1N-MCP or mApple-MCP to the lysates (Promega, L4960) for effector protein translation ...
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The recombinant plasmid was digested with Sac1 and Nde1 restriction enzyme and sub-cloned in pGL3 Basic luciferase vector (Promega, Madison, USA).
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bioRxiv - Developmental Biology 2022Quote: ... β-galactosidase assays were performed with the same cell extract using the β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer (Promega, #E2000). The luciferase activity was normalized against the lacZ activity of each culture ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Microbiology 2020Quote: ... Transfection efficiency was normalized through co-transfection of a lacZ reporter construct and determination of β-galactosidase activity using a commercial β-galactosidase enzyme assay system (Promega).
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... Media samples were taken to detect the secreted alkaline phosphatase reporter (GRE-SEAP) before the cells were lysed and assayed for β-galactosidase activity using the β-galactosidase enzyme assay system (Promega) to correct for differences in transfection efficiency ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were digested and ligated into the BglII (AGATCT) and MluI (ACGCGT) restriction enzyme sites of the pGL3-Promoter luciferase vector (Promega; Madison, WI). The ligated products were transformed into chemically competent DH5α cells using ampicillin (100mcg/ml ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The PCR products were digested and ligated into the BglII (AGATCT) and MluI (ACGCGT) restriction enzyme sites of the pGL3-Promoter luciferase vector (Promega; Madison, WI). The ligated products were transformed into chemically competent DH5α cells using ampicillin (100mcg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Immunology 2023Quote: ... L-lactate concentration was measured in CM as well as in supernatants of CM stimulated macrophages using an enzyme-based bioluminescent assay according to the manufacturer’s protocol (Lactate-Glo™ Assay, Promega GmbH, Germany). CM and supernatants were used 1:50 (planktonic CM ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Microbiology 2024Quote: ... The beads were washed thrice with 180 µl 80 % (v/v) ethanol prior to the addition of the digestion enzyme mix (0.6 μg of trypsin (V5111; Promega, Madison, WI, USA) and 0.6 µg LysC (125-05061 ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Cytochrome B was amplified from the same saliva sample in which DrDV-B was detected using primers Bat 05A and Bat 04A (45) and GoTaq Green Master Mix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Pre-B Cell viability was assessed using trypan blue dye exclusion test54 and the CellTiter-Glo® assay (Promega, G7570). For the CellTiter-Glo assay ...
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bioRxiv - Immunology 2020Quote: The strategy for genome-engineering to tag HLA-B with the HiBit peptide was developed based on a previous publication from Promega Corporation ...
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bioRxiv - Microbiology 2023Quote: ... Specific adapters 1 and 2 described in Table B were self-hybridized and then ligated to the ‘A’ tail ends by T4 ligase (Promega). After purification with AMPure XP purification kit (Agencourt) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein for the 4-methylumbelliferyl-b-D-glucuronide (MUG) and luciferase (LUC) assays was extracted in 1x passive lysis buffer (PLB: Promega). MUG assays were performed by adding 40 mL of protein extract to 100 mL of MUG assay buffer [2 mM MUG ...
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bioRxiv - Microbiology 2021Quote: ... A pT7 plasmid encoding a messenger RNA with a firefly reporter gene was used to generate control mRNA for translation assays (Promega, Madison, USA), herein known as pT7 Fluc ...
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bioRxiv - Microbiology 2023Quote: Viral capped mRNA (rat HEV) was made from linearized DNA using the Promega Ribomax Large Scale RNA Production System SP6 (Promega PRP 1300) and ARCA CAP (TriLink Biotechnologies) ...
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bioRxiv - Cell Biology 2021Quote: ... LgBiT antibody (N710A) and HiBiT antibody were acquired from Promega. Chicken polyclonal MAP2 (ab5392) ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
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bioRxiv - Molecular Biology 2021Quote: ... antibody was detected using HRP-conjugated chicken secondary antibody (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated rabbit secondary antibody (Promega). Anti-Beta Tubulin III (Tuj1 ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...
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bioRxiv - Bioengineering 2019Quote: CYP3A4 and CYP2C9 enzyme activities were measured by incubating the cultures with luciferin-IPA or luciferin-H substrates (Promega Life Sciences, Madison, WI) for 3 hours ...
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bioRxiv - Neuroscience 2021Quote: ... plates were removed from the incubator after 70 hours and 10 µL of 4x RT-Glo substrate and enzyme per well (Promega, Madison, WI, USA) diluted in P/S free medium ...
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bioRxiv - Cell Biology 2021Quote: Cytochrome P450 3A4 (CYP3A4) enzyme activity assays were assessed by measurement of luciferase activity using P450-Glo CYP3A4 assay kit (Promega, Madison, WI, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and digested with trypsin at an enzyme-to-substrate ratio of 1:50 (wt/wt, modified sequencing grade, Promega, Madison, Wisconsin. United States) for 16 hours at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... pVHV-SARS-CoV-2-SpikeWT and pVHV-SARS-CoV-2-SpikeOpt replicon vector DNA and helper DNA plasmids were linearized with NotI restriction enzyme prior to in vitro transcription using RiboMAX™ Express T7 RNA polymerase and cap analog (Promega, Madison, WI). Importantly ...
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bioRxiv - Plant Biology 2023Quote: ... following the instructions of the supplier and the digested vector was dephosphorylated by TSAP enzyme to avoid its recircularization (Promega, Madison, WI, USA). After electrophoresis ...
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bioRxiv - Immunology 2023Quote: T-cell and prodrug cytotoxicity assays with secreted enzymes were performed after 24 to 48 hours using CellTiter-Glo (Promega, Madison, Wisconsin, USA) or luminescence using firefly (ffLuc ...
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bioRxiv - Cancer Biology 2023Quote: ... The β-Galactosidase activity was measured in 30 µl of lysate incubated with 20 µl of reporter lysis buffer and 50 µl of 2X β-gal Enzyme Assay buffer (Promega, Cat# E2000). The absorbance was read in iMark microplate reader (BioRad) ...
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bioRxiv - Immunology 2023Quote: ... Secondary antibodies (Promega) were incubated for 1 h at RT ...
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bioRxiv - Pathology 2020Quote: ... cDNA synthesis from the mRNA was performed using a Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Promega, Madison, Wisconsin, USA) in a Mastercycler® Gradient (Eppendorf ...
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bioRxiv - Pathology 2021Quote: ... The DNA-A and DNA-B fragments were purified and cloned into the pGEM-T Easy vector (Promega, Madison, WI, USA) and then transformed into Escherichia coli strain DH5α cells by the heat shock method ...
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bioRxiv - Immunology 2021Quote: MSCs and B cells proliferation were measured using the CellTiter 96® AQueous cell proliferation assay kit from Promega (Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Next nuclei were pelleted and the transposition reaction was performed incubating the lysate for 30 min at 37 °C under agitation in the presence of Transposition mixture (Tris-HCl pH 7.6 10 mM, MgCl2 5 mM, dimethyl formamide 10%, Tn5 enzyme 100 nM – Illumina #20018704, digitonin 0.01% - Promega #G9441, Tween-20 0.1%, PBS 33%). The transposition reaction was purified (Qiagen MinElute PCR Purification kit ...