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Citations for Promega :
201 - 250 of 764 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... primers (0.4μM) and 0.75U of Go Taq® DNA polymerase enzyme (Promega Madison, Wisconsin, USA) with its buffer (1x) ...
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bioRxiv - Microbiology 2021Quote: ... restriction enzymes and polymerase were used as recommended by manufacturers (Promega and New England Biolabs). Plasmids were verified by PCR and Sanger sequencing (Eurofins) ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was reverse transcribed into cDNA using random hexanucleotide primers and M-MLV enzyme (Promega). ChIP experiments were performed using 20μg anti-ΔNp63 (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was reverse transcribed into cDNA using random hexanucleotide primers and M-MLV enzyme (Promega). Quantitative RT-PCR was performed with SYBR Green mix (ABgene ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids pKHNH6 and pKHNH3 were linearised prior to transformation using restriction enzymes EcoRV (Promega, USA) and KpnI (Fisher ...
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bioRxiv - Systems Biology 2023Quote: ... 1 µL of enzyme mix (lysC & trypsin, 10 ng/µL in water, Promega, Cat. V5072) was dispensed into each well by using the pL-volume dispensing function of the cellenONE at 20°C and 85% humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... at an enzyme-to-substrate ratio of 1:20 and protease max 0.1% (Promega, UK). Digestion was carried out overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: PolyA-enriched RNA was extracted using PolyATract mRNA isolation systems (#Z5310 Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNAs were in vitro transcribed (T7 RiboMAX Express Large Scale RNA Production System, Promega, USA) from the Nluc PCR template ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNAs were transcribed in vitro (T7 RiboMAX Express Large Scale RNA Production System, Promega, USA) from the linear fragment of the pET28-MVHL-UAA plasmid containing the T7 promoter ...
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bioRxiv - Developmental Biology 2021Quote: ... the 5’-end of each mRNA was capped using the Ribo m7G Cap Analog (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase (FLuc) mRNA was transcribed from the Luciferase T7 Control DNA plasmid (Promega) linearized using XmnI and in vitro transcribed using the mMessage mMachine T7 Kit (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of the resulting mRNA was mixed with 1 μl of FluoroTect GreenLys (Promega) and 3 μl of the wheat germ extract and then transferred under 50 μl of the translation buffer (1x SUB-AMIX SGC ...
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bioRxiv - Immunology 2020Quote: In vitro transcribed (IVT) mRNA was synthesized using RiboMAX T7 Kit (Promega, Madison, WI, USA) as described (37 ...
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bioRxiv - Developmental Biology 2023Quote: ... Artificial mRNA (synthetic RNA) was transcribed in vitro using SP6 polymerase (Promega, Madison, WI, USA) after NotI digestion (New England Bio Labs ...
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bioRxiv - Biochemistry 2023Quote: ... coli HY Kit used to synthesize EmGFP mRNA were purchased from Promega (Cat. No. L4380) and Biotechrabbit (Cat ...
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bioRxiv - Cell Biology 2024Quote: ... Prior to imaging of mRNAs cells were exposed to 200 nM JF646 HaloTag ligand (Promega) for 20 mins ...
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bioRxiv - Cancer Biology 2022Quote: ... and inserted into the XhoI restriction enzyme site of pNL1.1[Nluc] vector (Cat. No. N1001, Promega), resulting in the construction of pNL1.1-NR0B1pro vector.
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bioRxiv - Cell Biology 2022Quote: ... was digested with the corresponding enzymes and ligated with amplified genes using T4 DNA ligase (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... using BglII/HindIII restriction enzymes and instered into corresponding sites of pGL4.12 (Promega, Madison, Wisconsin, USA). A 902bp DNA fragment centered on rs11172113 ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Neuroscience 2023Quote: ... and 50 mM Tris-HCl at pH 7.9) containing 600 units of the CIAP enzyme (Promega). Tissues were then washed in DM and heat-induced antigen retrieval (HIAR ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with 1 μg/μL of RNA concentration using MMLV-RT enzyme (Promega, USA). HvHsfA6a gene specific and Actin primers designed by using Primer express 3.0 software (Thermoscientific USA ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was synthesized using RiboMAX™ Large Scale RNA Production Systems-T7 (Promega, Madison, WI, USA). For efficient translation of the fusion proteins in embryos ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantification of mRNA was performed using the BRYT Green® Dye (GoTaq® qPCR, #A600A, Promega) in a real-time PCR analysis system (StepOnePlus ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Bioengineering 2023Quote: ... was used to transcribe luciferase mRNA from the linear luciferase DNA template (1,800 bases, Promega, UK). Generally ...
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bioRxiv - Immunology 2021Quote: ... B.1.351) with TMPRSS2 into 293T cells using Fugene 6 transfection reagent (Promega, Madison, WI)54–56 ...
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bioRxiv - Systems Biology 2020Quote: ... Enzyme activity was determined using the UDP-Glo™ or UMP/CMP-Glo™ Glycosyltransferase Assay (Promega) that determined UDP/CMP concentration formed as a by-product of the glycosyltransferase reaction ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Enzyme activity was assessed by SAH production as determined by luminescence through the MTase-Glo assay (Promega). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by A-tailing reaction using KapaTaq enzyme and cloned in pGemT-Easy vector system (Promega™) or TOPO TA cloning vector as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was digested with the same enzymes and ligated with the amplified receptors using T4 DNA ligase (Promega).
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bioRxiv - Immunology 2020Quote: ... To normalise transfection efficiencies β-Galactosidase levels were measured using the β-Galactosidase Enzyme Assay System (Promega). 20 μl of lysate was diluted 1:1 in 1x reporter lysis buffer before addition of 40 μl 2x assay buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested with the BamHI and Sall restriction enzymes and inserted into pGEM4Z (Promega) that had been digested with the same enzymes ...
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bioRxiv - Biochemistry 2021Quote: ... for 1 h at 37°C (sample to enzyme ratio 1:20) and then with GluC (Promega) (1:40 ratio ...
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bioRxiv - Microbiology 2022Quote: ... 25 ng of DNA were mixed with 12.5 ul of Go-Taq green master mix enzyme (Promega) and 10 uM of each primer (supplementary table 1) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Systems Biology 2024Quote: ... 75 µl of digestion buffer containing 0.8 µg Trypsin Gold (enzyme to protein ratio 1:25, Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Molecular Biology 2021Quote: ... and PolyA mRNA from two rounds of oligo dT selection was prepared according to the manufacturer (Promega). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... The NanoLuc mRNA was in vitro transcribed (T7 RiboMAX Express Large Scale RNA Production System, Promega, USA) from a template containing β-globin 5’-UTR ...
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bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
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bioRxiv - Biochemistry 2022Quote: Target mRNA was prepared by transcribing DNA using T7 RNA polymerase according to the manufacturer’s instructions (Promega). In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System ...
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bioRxiv - Bioengineering 2023Quote: ... We measured mRNA expression by bioluminescence assay using Nano-Glo® Luciferase Assay System (Promega, Catalog # N1110). Briefly ...