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Citations for Promega :
301 - 350 of 764 citations for Apolipoprotein B mRNA Editing Enzyme Catalytic Subunit 3G APOBEC3G Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and digested for 2 hours at 37°C with ApaI and SpeI restriction enzymes and ligated with T4 DNA ligase (Promega) overnight at 16° to a double-digested pKNG101 plasmid ...
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bioRxiv - Genomics 2021Quote: ... Inserts were cut out with appropriate restriction enzymes (Suppl. Table 1) and ligated with pre-digested pGL4.23[luc2/minP] (Promega, # E8411) plasmid upstream of a minimal promoter and a firefly luciferase gene ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then digested in-gel by rehydrating the gel slices in trypsin enzyme solution consisting of 6ng/µl trypsin (Promega) in 50mM ammonium bicarbonate solution ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
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bioRxiv - Cell Biology 2022Quote: ... Cloning into the mammalian pSVL expression vector was performed using restriction enzymes BamHI and XhoI followed by T4 DNA ligase (Promega) ligation ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified coding sequence was then subcloned into the pGHJ vector after double digestion with SmaI e EcoRI restriction enzymes (Promega). hTGR5 gene was in pCMV6-Entry (GPBAR1 Human cDNA ORF Clone ...
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bioRxiv - Molecular Biology 2020Quote: ... All psiCHECK2 constructs were generated by cloning sequences synthesized and cloned in pUC57 (Genescript) into psiCHECK2 using XhoI and NotI restriction enzyme sites (Promega).
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bioRxiv - Microbiology 2022Quote: ... was added to a final concentration of 3 mM followed by the addition of 5 µl of T7 Enzyme Mix and 50 U RNasin RNase Inhibitor (Promega). To produce transcripts for mock transfections the GTP concentration was increased to 7.5 mM and cap analogues were not added ...
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bioRxiv - Molecular Biology 2020Quote: ... 20μg of total cell extracts each from heavy and light SILAC labels were combined and diluted to a final urea concentration of 1M and then digested overnight with LysC enzyme (Promega) at a 1:100 (enzyme:protein ...
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bioRxiv - Microbiology 2020Quote: ... towards HeLa cells was determined as a release of the intracellular enzyme lactate dehydrogenase (LDH) into the cell culture media using CytoTox 96 assay (cat.no. G1780, Promega, USA) or as changes in cell membrane integrity using CellTox Green Cytotoxicity Assay (cat.no ...
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bioRxiv - Genetics 2020Quote: ... these primers were also designed with restriction enzyme site overhangs (BamHI and HindIII) to facilitate cloning into the pGEM-1 plasmid (Promega) (Fig ...
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bioRxiv - Genetics 2020Quote: ... The primers were designed with restriction enzyme site overhangs (BamHI and HindIII) to facilitate cloning into the pGEM-1 plasmid (Promega) (Fig ...
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bioRxiv - Microbiology 2020Quote: Cytotoxicity of macrophages after 8 hours of infection was quantified by measuring the release of the enzyme lactate dehydrogenase in cell supernatants using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... with restriction enzyme overhangs were ordered from Genewiz and cloned into pGL3-basic plasmid upstream of the firefly reporter gene (E1751, Promega). Minipreps were prepared with QIAprep Spin Miniprep kit (Qiagen) ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was used for first-strand cDNA synthesis using Moloney murine leukemia virus (MMLV) reverse transcriptase enzyme (Promega, USA) and random primers (Biodynamics ...
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bioRxiv - Plant Biology 2022Quote: ... were reduced with DTT and then alkylated with iodoacetamide and digested overnight using Lys-C/Trypsin (1:50, enzyme to protein; Promega). After terminating the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was measured combining by combining 20 μl of sample with 50 μl of enzyme substrate (Luciferase 1000 Assay System, #E4550, Promega), vortexing sample briefly and counting photons for 5 s in the luminometer (Luminoskan TL plus ...
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bioRxiv - Molecular Biology 2022Quote: ... REBOV Vp40- pGEM-T Easy and MERS-CoV UpE-pGEM-T Easy were first linearized by Sal I enzyme (Promega) creating a 5’overhang ...
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bioRxiv - Molecular Biology 2022Quote: The 3’ UTR of the human MAD2L1 with predicted miR-binding site was amplified from HCT116 genomic DNA and cloned to the psiCHEK2 located downstream of the Renilla translational stop codon into XhoI/NotI restriction enzymes (Promega). 3’UTR mutant was obtained by using the Site Directed Mutagenesis (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... and then reverse transcribed into complementary DNA (cDNA) using 30 enzyme units(U) of the MMLV-RT (Promega, WI, USA) and 1μM of the labeled primer ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Developmental Biology 2024Quote: pCS2+ expression vectors carrying membrane-localized fluorescent CAAX-GFP (mGFP) or Utrophin-RFP (utr-RFP) were linearized using restriction enzymes (Promega) for two hours at 37°C and precipitated at -20°C overnight in 70% ethanol ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-m6A conjugated beads were incubated with purified mRNA with rotation at 4°C overnight in 300 µL MeRIP buffer with 1 µL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
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bioRxiv - Plant Biology 2021Quote: Total mRNAs used for qRT□PCR experiments were isolated from Chlamydomonas using the Eastep Super total RNA extraction kit (Promega), and cDNA was synthesized with the PrimeScript TMRT Master Mix kit (TaKaRa) ...
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bioRxiv - Neuroscience 2022Quote: DNA constructs with different WT and mutated 5’UTRs of mouse Sox2 mRNA were assembled in the psiCheck-2 vector (Promega) including synthetic Renilla luciferase gene (hRluc ...
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bioRxiv - Bioengineering 2020Quote: ... Cas13a mRNA was translated in vitro using the Rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, WI, USA). RNaseAlert®-1 Substrate was mixed with crRNA or NTCR (500 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μL in vitro translation reactions were performed in the linear range using 2.8 nM mRNA in the Flexi RRL System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Immunology 2020Quote: ... anti-m6 A conjugated beads were incubated with purified mRNA with rotation at 4C overnight in 300 mL MeRIP buffer with 1 mL RNase inhibitor (recombinant RNasin; Promega). 10% of the mRNA sample was saved as the input fraction ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...
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bioRxiv - Physiology 2023Quote: ... 300 μl aliquots from each fraction after polysomal ultracentrifugation in sucrose density gradients were spiked with 100 pg of Luciferase mRNA (internal control, Promega). Then ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Cell Biology 2022Quote: ... The mRNAs were produced by in vitro transcription using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Poly(A) containing RNAs were separated from the RNA pool using a Poly(A) mRNA isolation system IV (PolyATract, Promega), followed by Ribozero purification ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Molecular Biology 2023Quote: ... were used in qRT-PCR reactions to determine the relative mRNA levels of Tc_wap genes using GoTaq® qPCR Master Mix (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1h ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...
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bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... All PCR fragments were digested with KpnI and HindIII restriction enzymes and ligated with pGL3-Basic vector (Promega, Madison, WI, USA).
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bioRxiv - Pharmacology and Toxicology 2023Quote: The experiments to evaluate the inhibitory activity levels of compounds were carried out according to the manufacturer’s instructions on the SYK Kinase Enzyme System (Promega, Wisconsin, USA) and ADP-GloTM Kinase Assay (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... RT-qPCR was performed to determine the mRNA levels of CD24 and EMT biomarkers (Table S3) using GoTaq® qPCR Master Mix with SYBR green (Promega). The qPCR was performed in a total volume of 20µL per-reaction containing ...
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bioRxiv - Microbiology 2021Quote: ... and the extracted mRNA was used as a template for reverse transcription using M-MLV reverse transcriptase (Promega, Madison, WI, USA). Virus was quantified using real-time PCR using SYBR Premix Ex Taq™ (Applied Biosystems ...