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Citations for Promega :
4101 - 4150 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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Cobalt(III) Schiff Base complexes stabilize non-fibrillar amyloid-β aggregates with reduced toxicitybioRxiv - Neuroscience 2020Quote: ... Cellular viability was assessed using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI). Cells were plated in 96-well tissue culture treated plates at a density of 5,000 cells/well ...
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bioRxiv - Immunology 2020Quote: ... and finally the luciferase activity in total cell lysates was measured with Glomax (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2020Quote: ... and cells were lysed with 120 μl of lysis buffer (ratio of medium and Steady-glo (Promega) at 1:1 ...
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bioRxiv - Neuroscience 2019Quote: PC12 cells were transfected with the indicated Firefly Luciferase-IMPA1 constructs and thymidine-kinase – Renilla Luciferase (Promega) using Lipofectamine 2000 for 48hrs ...
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bioRxiv - Biochemistry 2021Quote: Histones extracted from HeLa cells were dissolved in 50 mM NH4HCO3 buffer and digested with trypsin (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The cytotoxicity was evaluated by the CellTiter-Glo® luminescent-cell viability assay kit (Promega; WI, USA) following manufacturer protocol ...
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bioRxiv - Microbiology 2021Quote: Cells were washed once with PBS and harvested in 100 ml of Renilla Luciferase Activity buffer (Promega). 10 μl aliquots were used to measure luminescence using the Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2019Quote: Cells were washed with PBS and lysed with 100 μl Renilla Luciferase Assay System Lysis Buffer (Promega). Cell lysates (20μl ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were analyzed after 30 h incubation for luciferase reporter activity with DualGlo luciferin reagent (Promega) using a luminometer (Thermo Fluoroskan Ascent FL) ...
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CCG•CGG interruptions in high penetrance SCA8 families increase RAN translation and protein toxicitybioRxiv - Genetics 2021Quote: Cell toxicity and viability were assessed 42hrs post-transfection using the CytoTox 96 Nonradioactive Cytotoxicity Assay (Promega) or 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT ...
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bioRxiv - Microbiology 2021Quote: ... ATP levels were measured in 100µl cells as described previously(12) using a BacTiter Glo kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... into HEK 293T cells using 59ul of FUGENE HD transfection reagent (Promega, San Luis Obispo, CA, USA) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Cytotoxicity after treatment with single and combined UPR inhibitors was measured using the Cell Titer Blue (Promega) and trypan blue exclusion kits (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells were pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells are pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2020Quote: ... HeLa cells were transiently transfected with the PX459–sgRNF213-exon3 vector using FuGENE 6 Transfection Reagent (Promega). Forty-eight hours post-transfection ...
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bioRxiv - Biophysics 2021Quote: ... Constructs (2 μg) were transfected into HEK293 cells on glass coverslips using Fugene HD (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were verified to be mycoplasma-negative and genetically authenticated by STR profiling (Gene Print 10, Promega). HBEKT cells were maintained in keratinocyte serum free media with supplementation according to Lonza protocol ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were transfected with CMV-EPO-eGFP-p2A-A1AT-mCherry using Fugene HD transfection reagent (Promega). Transfected cells were selected with 350µg/mL hygromycin (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The identities of the T24 and HeLa cell lines were authenticated by PowerPlex16 STR (contracted to Promega). All of the cells used were mycoplasma free ...
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bioRxiv - Cancer Biology 2021Quote: ... cell viability and Caspase activity were detected with CellTiter-Glo 2.0 and CaspaseGlo-3/7 assay (Promega) respectively 2 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... and plates were incubated for 5 days before being assayed using the Cell Titer-Glo kit (Promega).
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bioRxiv - Biophysics 2020Quote: ... supernatants were harvested for virion purification and monolayers were lysed in cell culture lysis buffer (CCLR, Promega) for immunoblotting ...
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bioRxiv - Cell Biology 2021Quote: Cellular viability was measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... RNA from laser-microdissected areas were extracted with a ReliaPrep RNA cell Miniprep kit (Promega, Madison, USA) according to the manufacturer’s protocol and quantified/qualified with Nanodrop and Agilent 2100 Bioanalyzer apparatus (RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained with different concentrations of TMR HaloTag ligand according to the HaloTag labeling protocol (Promega) and 50,000 cells were analyzed by flow cytometry (BDLSR Fortessa ...
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bioRxiv - Genomics 2020Quote: ... Total genomic DNA was extracted from the pelleted cells using Wizard DNA purification kit (Promega, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Cells were lysed 24 hr after transfection and analyzed with Dual-Luciferase® Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The transfected cells were labelled with HaloTag TMR ligand following manufacturer’s protocol (HaloTag® TMR Ligand Promega). Nuclear extract from transfected HEK293 cells (Halo-SP140 ...
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bioRxiv - Immunology 2021Quote: ... TCR- and CD3-encoding pMIG II vectors were used to transiently transduce HEK293T cells using FUGENE (Promega). Cells were harvested after 48h and assessed for CD3/TCR expression and CD1d-tetramer binding using flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection was done on pre-seeded HeLa cells (70% confluency) with the FuGENE 6 Transfection Reagent (Promega) following standard protocol ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested and genomic DNA (gDNA) was extracted using the Wizard Genomic DNA Purification Kit (Promega). Quantification was performed with a BioDrop µLITE (BioDrop ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then harvested and luciferase activity was measured using the dual luciferase reporter assay reagents (Promega), as per the standard protocol ...
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bioRxiv - Microbiology 2021Quote: ... 100 μl of cell culture supernatant was replaced with 100 μl CellTiter-Glo® (Promega, Madison, WI) and incubated for at least 10 min at room temperature prior to measuring luminescence on a Perkin Elmer (Waltham ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells from the host shutoff assays above were harvested and lysed in passive lysis buffer (Promega) for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell growth was stopped by adding CellTiter-Glo as per manufacturer’s instructions (Promega, Madison, WI, USA; G7570). Data analysis was performed by normalisation on the vehicle treated of the respective plate set as = 100 ...
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bioRxiv - Cell Biology 2022Quote: eGFP-HSP27 immunoprecipitations were performed in p62KO HeLa cells 24hrs following transient transfection using Fugene 6 (Promega). HeLa cells were treated with 750µM LLOMe for 2hrs prior to immunoprecipitation ...
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bioRxiv - Cell Biology 2022Quote: ... The transfection of wild-type MCF10A cells and the mutated derivatives was carried out with FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from BMDCs using the ReliaPrep RNA Cell Miniprep System (#Z6012, Promega, Madison, USA) and from the skin using ISOGEN (#311-02501 ...
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bioRxiv - Molecular Biology 2022Quote: Cell viability was determined by a luminescent assay according to the manufacturer’s instructions (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... After 18h incubation time cell viability was measured by adding 20 μl CellTiter-Glo 2.0 Reagent (Promega). Luminescence signals of the CellTiter-Glo assay were detected on the EnVision 2104 Multilabel plate reader (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was produced as described before.25 Sf21 cells were transfected with bacmids using Fugene6 (Promega #E2693). V0 virus containing media was harvested after 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... The viability was measured using the CellTiter-Blue Cell Viability Assay (Promega, Catalog number: G8080 or G9242) following manufacturer instructions.
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bioRxiv - Developmental Biology 2022Quote: Cells were prepared for Luciferase and Renilla measurements using the Dual-Glo Luciferase Assay System (Promega, #E2920) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and aliquots of the cells were analyzed by genomic PCR using GoTaq DNA polymerase (Promega, WI, USA) and specific primers (F_C2 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA extraction from cells was performed using the Maxwell RSC simplyRNA Tissue Kit (Promega, Madison, WI, USA) as according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were harvested and luciferase was measured using a Dual-Luciferase Reporter Assay System (Promega, E1980). The promoter and enhancer activity were calculated by the ratio of firefly/renilla luciferase activity.
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bioRxiv - Cancer Biology 2022Quote: ... Following 5 days of drug incubation cell viability was assayed using 30μl of CellTiter-Glo® (Promega). Screens were performed in technical triplicate.
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bioRxiv - Genomics 2022Quote: ... cells in each well were first lysed with 50μL ice-cold 1X passive lysis buffer (Promega E1941), allowed to incubate on an orbital shaker for 10 minutes at room temperature ...