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Citations for Promega :
301 - 350 of 1739 citations for Recombinant Human PRL Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... protein was modified with an poly(A)25 insertion at the 3’ end of the gene within the pRL-null vector (Promega). The gene encoding the NanoLuc luciferase was transferred from the pNL1.1 vector (Promega ...
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bioRxiv - Cell Biology 2021Quote: HeLa cells cultured in 24-well plates were transfected with indicated firefly luciferase reporter construct (SBE×4-luc or BRE-luc) together with pRL-SV40 renilla luciferase control vector (Promega) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The WT or mutant plasmids were then transfected into WT and SIRT1 KO mESCs together with the control pRL-TK plasmid (Renilla Luciferase, Promega). Cells were cultured for 48 h and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were co-transfected with the plasmid pGL4.17 containing a YB-TATA-based minimal activity promoter and enhancer sequences (GGAA-msats of various lengths, genomic or synthetic alk-SE sequences) and pRL Renilla Luciferase Control Vector (Promega) (plasmid mass ratio of pGL4.17:pRL = 100:1) ...
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bioRxiv - Neuroscience 2021Quote: ... were transfected using Lipofectamine 3000 with each construct (400 ng) and the Renilla luciferase expression vector pRL-TK (40 ng; Promega) in triplicate ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Immunology 2022Quote: A total of 200,000 RAW cells were seeded on a 24-well plate and were co-transfected with NFATc1(pGL3-NFAT) and Renilla luciferase plasmid (pRL-TK, Promega) in a ratio of (2:1) ...
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bioRxiv - Plant Biology 2022Quote: ... and 5 μg plasmid DNA was co-transfected with same amount of low-rate constitutively expressed pRL-TK Renilla luciferase vector (Promega) into mesophyll protoplasts isolated from A ...
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bioRxiv - Immunology 2020Quote: ... HF and MEF cell lines were transfected with 10 ng of the internal control plasmid pTK-Renilla (pRL-TK, Promega) or 60 ng of the reporter plasmid pLUC-NF-κB (R ...
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bioRxiv - Developmental Biology 2019Quote: ... The University of Queensland) constructs were co-transfected (at ratio of 1:10) with renilla luciferase (pRL-TK vector, PROMEGA) in Non Targeting Control or H2a.z.2 siRNA treated B16 melanoma cells ...
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Biophysics 2022Quote: HEK293 cells with stably integrated firefly-luciferase-based Wnt reporter (TOPbrite) (Zhang et al., 2009) and pRL-SV40 Renilla luciferase (Promega) were maintained in a 5% CO2 humidified incubator at 37°C in DMEM with nutrient mixture F12 (50:50) ...
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bioRxiv - Cancer Biology 2022Quote: ... The wells were co-transfected with different PFKFB3-HRE luciferase constructs along with pRL-TK renilla luciferase plasmid (Promega, E2231). 12hrs post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... S2-VP10 cells were co-transfected with 0.5 μg of each luciferase reporter plasmid and 1 ng of pRL-SV40 (Promega, E2231) the day after plating (3.45 × 104 cells/well in a 24-well plate ...
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bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Flag-tagged STAU1 or STAU1RBDΔ3 or Flag plasmids with the wildtype or mutant 5’UTR-MTOR-LUC or luciferase empty vector constructs and Renilla luciferase plasmid (pRL-SV40 vector, Promega) according to an experimental set-up ...
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bioRxiv - Biochemistry 2022Quote: ... with 10 ng pGL4.32[luc2P/NF-κB-RE/Hygro] (NF-κB response element-dependent firefly luciferase) or pGL4 [luc2P/AP-1-RE/Hygro] and 5 ng pRL-TK using the Dual Luciferase Reporter Assay (Promega). 24 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... we transfected 500,000 ANBL6 cells with 1 ug of reporter plasmid plus 250 ng of Rinella plasmids pRL-SV40 and pGL3 control (Promega) in biological triplicates ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 49 ng of a TP1-Luciferase (Kurooka et al., 1998; Minoguchi et al., 1997) and 1 ng Renilla-Luciferase (pRL-TK, Promega) using Lipofectamine™ 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Double transfections were performed using 200 ng total DNA containing 100 ng of Fluc reporter vector and 100 ng of Renilla luciferase (Rluc) vector (pRL-TKl; Promega). After 4 hours of transfection ...
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bioRxiv - Genetics 2024Quote: ... Cells were co-transfected after 24 hours with 50 ng of the CNTNAP5 luciferase reporter constructs along with 10 ng of pRL-TK Renilla luciferase internal control using FuGENE HD transfection reagent (Promega). At 48 hours post-transfection ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Developmental Biology 2019Quote: ... Tagged-β-Catenin and Tagged-β-Catenin mutants were synthesized using the TNT coupled reticulocyte lysate system according to the manual (Promega, L5020, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... For these experiments pGL2M4-luc reporter plasmid37 (containing four CACGTG binding sites, a canonical E-box) and pRL Renilla Luciferase Control Reporter Vectors (CMV, Promega E2231) were used ...
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bioRxiv - Cell Biology 2019Quote: ... University of Wisconsin), and 1ng HSV-thymidine kinase promoter driven Renilla luciferase (pRL-TK-Renilla, referred to as Renilla) (Promega, E2241) per well using Lipofectamine 2000 (Life technologies ...
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bioRxiv - Genetics 2022Quote: ... was transiently transfected with the pRL-TK Renilla internal control vector (50 ng) using 4 μL Viafect™ transfection reagent (Promega) into suspension cell lines or adherent cell lines ...
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bioRxiv - Immunology 2020Quote: ... 50 ng of an IFNβ promoter reporter plasmid (p125-luc) and 20 ng of a Renilla luciferase reporter (pRL-TK; Promega E2241) under control of a thymidine kinase promoter were co-transfected ...
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bioRxiv - Molecular Biology 2022Quote: ... The luciferase construct of interest was co-transfected with or without the relevant Gal4-TAD and the pRL Renilla control (Promega, E2261). Cells were harvested 48hrs after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... AAA start codon variants) was constructed by subcloning RLuc from pRL-SV40 (which was a kind gift from Aaron Goldstrohm; Promega # E2231) via PCR amplification to insert the N-terminal 3XFLAG tag ...
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bioRxiv - Microbiology 2023Quote: ... pNL4.3.Luc.R-.E– and pRL Renilla were collected 48 hours post-nucleofection and processed for luciferase readings using Dual-Glo® Luciferase Assay System (Promega) following manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated antigens were immobilized using streptavidin-coated magnetic beads (Promega). In each round ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated RNAs were treated with RNase-free DNase I (Promega), purified on G-50 MicroSpin columns (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
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bioRxiv - Biochemistry 2021Quote: ... 10 units of recombinant RNAsin (Promega®) and 1 unit/μl Avian Myoblastosis virus reverse transcriptase (Promega® ...
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Regulation of skeletal muscle metabolism and contraction performance via teneurin-latrophilin actionbioRxiv - Physiology 2021Quote: ... Ultra-Glo recombinant luciferase (Promega, Wisconsin, USA) was added to the media to determine ATP levels ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant RNase inhibitor (0.2 U/μl; Promega, 2% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase refolding assay: QuantiLum Recombinant Luciferase (Promega) was diluted to 55 µM in denaturation buffer containing 6M Guanidium/HCl and 1 mM DTT during 30 minutes at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 1U/μL Recombinant RNase inhibitor (Promega, PAN2515), and 0.1% Triton X-100) ...
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bioRxiv - Biophysics 2023Quote: Transfected cells were stained with JF-549 tagged HaloTag ligand (Promega) and Vybrant DiO staining solution (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... HALO-tagged miniGs was labeled using HALO-JaneliaFluor595 (HALO-JF595) (Promega) by incubating cells in ∼2 μM of HALO-JF595 in DMEM supplemented with 10% FBS for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... with 200 ng of miR-211 overexpression construct or empty vector along with 5 ng of pRL-CMV plasmid vector encoding Renilla luciferase gene using FuGENE transfection reagent (Promega, Madison, WI). After 48 h incubation ...
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bioRxiv - Genomics 2022Quote: The 293T cells in 96-well plates were transiently transfected with 200 ng Firefly luciferase vector (pGL3) and 4 ng pRL-TK Renilla luciferase vector (Promega, Madison, USA) using 0.5 uL Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega, Cat. #E2241) using Lipofectamine®2000 transfection reagent (Thermofisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the CMV Promotor of pCDNA3 2xMyc-Su(H)-VP16 was replaced by the HSV-TK Promotor of the pRL TK Vector (Promega, Madison, USA). To this end ...
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bioRxiv - Immunology 2023Quote: ... pNF-kB-Luc that has the NF-kB promoter linked to firefly luciferase and a plasmid that constitutively expresses Renilla luciferase from the thymidine kinase promoter (pRL-TK-Luc) were purchased from Promega (Madison, WI). pRL-TK-Luc serves as an internal control to test the efficiency of transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 μg/well of either pGL3-B.HTSS2KB or pGL3-Intron3-DSCR1 reporter construct was transfected with 150 μg/well of pCDNA3-ΔCnA,and 300 μg/well pEF-BOS-hNFATp and/or 150 until 600 μg/well of pEGFP-VIVIT.7,17 pRL-TK (Promega; xx μg/well), containing the thymidine kinase promoter driving Renilla luciferase ...
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bioRxiv - Biochemistry 2020Quote: ... biotinylated RBD-T was immobilized on the Streptavidin MagneSphere particles (Promega) and added to the solution containing phage ...
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bioRxiv - Cell Biology 2021Quote: ... PolyA mRNA was detected using biotinylated oligo-dT probes (Promega #Z5261). Probe was diluted in ULTRAhyb-Oligo Hybridization Buffer (0.5 µL of 5 pmol/uL per mL of buffer) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 µl of 50 pmol/µL biotinylated oligo-dT probes (Promega), and 408 µl of diffusion buffer (3X Saline-Sodium Citrate (SSC) ...