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Citations for Promega :
201 - 250 of 1739 citations for Recombinant Human PRL Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 0.8 μg of the indicated reporter constructs and 0.4 μg of pRL-TK (Promega), as an internal control of transfection efficiency ...
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bioRxiv - Cell Biology 2021Quote: ... The empty pGL3-Basic was used as a negative control and pRL-CMV (Promega) was used as an internal control.
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bioRxiv - Biochemistry 2021Quote: ... 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK, Promega), and 40 ng ml-1 of pLEXm vector (34 ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 50 pg of the pGL4.23 luc2/miniP enhancer:luciferase plasmid and the following amounts of MOs or mRNAs into each dorsal marginal zone (dmz ...
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bioRxiv - Cancer Biology 2023Quote: ... 15 ng of pRL-null Renilla luciferase control reporter (#E2271, Promega; for background normalization), and compensated with empty vector control (pCMV2c ...
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bioRxiv - Biochemistry 2023Quote: ... The pGL3-ISRE-Luc vector and the pRL-CMV vectors were obtained from Promega.
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Microbiology 2021Quote: ... HA-tagged DDX1and HA-tagged TRIF were constructed by inserting indicated sequences into pCMV-HA (Promega), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Biophysics 2021Quote: ... Twenty-five ng of a plasmid encoding Renilla luciferase under CMV promoter (pRL-CMV, Promega) was co-transfected for normalization purposes ...
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bioRxiv - Developmental Biology 2021Quote: ... 25 ng or 50 ng pRL-SV40 Renilla luciferase reporter vector (Promega, Madison, WI, USA) was co-transfected ...
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bioRxiv - Genomics 2022Quote: ... The vectors were co-transfected with the pRL-TK Renilla luciferase control reporter vector (Promega) for normalization purposes ...
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bioRxiv - Immunology 2023Quote: ... 1μg of pGL4.10 test plasmid and 0.01μg of pRL-TK control plasmid (Promega Cat. E2241) were added per well ...
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bioRxiv - Microbiology 2023Quote: ... 800 ng pNL4.3.Luc.R-.E– (NIH Reagents program) and 200 ng pRL Renilla (Promega E2231) as control for nucleofection efficiency ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Plant Biology 2023Quote: Myc- and FLAG-tagged versions of the different ALOG proteins were produced using the Quick Coupled Transcription/Translation System (TnT; Promega). 25 µL of TnT reactions producing indicated proteins were mixed with Buffer 1 to reach 150 µL and rotated at 4 °C during 1 h ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Neuroscience 2023Quote: Nine DIV hippocampal slice cultures were randomly assigned to treatment groups with the following drugs dissolved in serum-free medium: recombinant human BDNF (250 ng/mL, Promega); BDNF + K-252a (200 nM ...
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bioRxiv - Systems Biology 2020Quote: ... albicans as well as of a commercially available human protein digest (Promega), which was exclusively used in this experiment ...
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bioRxiv - Biochemistry 2023Quote: MS-Compatible Human Protein Extract Digest (K562) was purchased from Promega (V6951), MassPREP E ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 10 minutes at room temperature with 0.35 µg of pRL-TK (Promega) and either 10 µg of each luciferase reporter vector or 5 µg of each reporter and 5 µg of an additional expression vector ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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bioRxiv - Genomics 2019Quote: ... 2 μg of each of these reporter plasmids along with 100 ng of pRL-TK (Promega), a Renilla luciferase expression plasmid to control for transfection efficiency ...
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bioRxiv - Cancer Biology 2021Quote: ... and pRL-TK vector (as an inner control that contains Renilla luciferase sequences (Promega; Madison, WI)) using Lipofectamine2000 transfection reagent (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... All luciferase constructs were co-transfected with pRL-TK Renilla luciferase plasmid (Promega, Madison, WI, USA) to normalize transfection efficiency variation between samples ...
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bioRxiv - Biochemistry 2021Quote: ... along with Renilla luciferase (50 ng of pGL4.70[hRluc] or 1 ng of pRL-null; Promega), and pCI expression vector encoding either FL-PC1 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transfected with 100 ng pGL3-RARE-luciferase and 10 ng pRL Renilla (Promega E2261) using Mirus TransIT-2020 Transfection Reagent (Mirus MIR 5404 ...
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bioRxiv - Cancer Biology 2022Quote: ... All luciferase assays were performed in triplicate and results were normalized to Renilla (pRL-TK; Promega).
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bioRxiv - Cell Biology 2022Quote: ... cells were co-transfected with the above BGT1 promotor constructs and pRL-TK (Promega, Cat# E224A). After osmotic stress treatment for 9 hours ...
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bioRxiv - Molecular Biology 2023Quote: SVG-A cells were transfected with a mixture of TP1-firefly luciferase and pRL-TK (Promega) plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Other plasmids were obtained from commercial vendors or from the Addgene repository: pRL-CMV-Renilla (Promega), pRS0045 (Addgene #131124) ...
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bioRxiv - Cell Biology 2021Quote: ... Janelia Fluor 646 tagged Halo ligand (Promega) was used at 200nM final concentration to label SINAPS-mRNA in cells prior to fixation/imaging.
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant (rTdT) enzyme (Promega). Cells were then incubated at 37 °C for 60 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
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bioRxiv - Molecular Biology 2019Quote: PUER cells were transfected with Cebpa promoter reporter vector and Renilla control vector pRL-CMV (Promega, E2261) in a 1:200 ratio using a 4D-Nucleofector (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: The resulting VTG-CpGL plasmid was co-transfected with a Renilla luciferase-expressing plasmid (pRL-SV40, Promega) into LMH/2A cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... of the relevant pCpGL-EF1 plasmid and 6ng (DNA methylation) or 1.5ng (SOX9 overexpression) of pRL-TK Renilla control plasmid and 0.3μl FuGeneHD reagent (Promega) per well ...
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bioRxiv - Cancer Biology 2019Quote: ... The luminescence values were transfection normalized with the internal control pRL-TK (50 ng, Renilla Luciferase; Promega). Experiments were performed with three biological replicates.
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bioRxiv - Cancer Biology 2020Quote: ... The 3’UTR fragments were cloned in the XbaI site of the pRL-CMV vector (Promega, #E2261) (pCMV-Luc-PTCH1 3’UTR wt/mut) ...
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bioRxiv - Cell Biology 2021Quote: ... or let7A2 generated in the laboratory and with 50 ng of pRL-TK Renilla luciferase plasmid (Promega). ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of 150 ng of pRL-TK-Renilla luciferase plasmid (Promega, Charbonnières-les-Bains, France). After 48h incubation with Doxycycline ...
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bioRxiv - Genetics 2023Quote: ... with 500 ng reporter and 50 ng pRL Renilla Luciferase Control Reporter vector (Promega Cat. No. E2231). Luciferase intensity was measured 24 hours after transfection with Dual-Luciferase® Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2023Quote: pISRE-Luc, pGAS-Luc (kindly provided by Eliane Meurs, Institut Pasteur) and pRL-TK Renilla Luciferase (Promega) plasmids were used for luciferase assays ...
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bioRxiv - Developmental Biology 2023Quote: ... 16/32-cell embryos were co-injected with 5 pg of pRL-TK:renilla luciferase plasmid (Promega E2241) + 80 pg of the pGL4.23 luc2/miniP HumanPDX1 enhancer:firefly luciferase plasmid (wt or 6RFX motif mutant ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...