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Citations for Promega :
151 - 200 of 1739 citations for Recombinant Human PRL Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... Firefly and pRL-SV40 renilla luciferase constructs (Promega, Genbank Accession # AF025845) were transfected using Solution SF (Lonza Catalog # V4SC-2096 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20 ng of the control pRL-TK Vector (Promega, E2241) into 293T cells using Lipofectamine 2000 Transfection Reagent (Themo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega), in the presence or absence of indicated expression vectors using FuGene 6 transfection reagent (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 ng of Renilla luciferase expressing pRL SV40 plasmid (E2231, Promega), as internal control ...
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bioRxiv - Cell Biology 2024Quote: ... was used to assay p53 transcriptional activity and pRL-CMV (Promega) was used as the transfection control ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... The Renilla luciferase expression vector pRL-TK (50 ng, Promega, Madison WI) was co-transfected for normalization ...
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bioRxiv - Developmental Biology 2021Quote: ... and 0.9 ng/well of pRL-CMV Renilla luciferase reporter plasmid (Promega) as a reference ...
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bioRxiv - Genomics 2019Quote: ... or an empty vector and 50ng of the vector pRL-SV40 (Promega) using the Lipofectamine 3000 reagent (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... the pRL-null construct encoding a Renilla luciferase gene (Promega, cat.no. E2231) was used ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... 50 ng PPRE X3-TK-luc and 6 ng pRL-SV40 (Promega), in the presence of the Fugene HD transfection reagent (Promega) ...
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bioRxiv - Genomics 2021Quote: ... cells were co-transfected with SEMA7A-Luc4 and pRL-CMV (Promega, # E2261) constructs and enhancer activity was measured with a luciferase assay.
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bioRxiv - Plant Biology 2021Quote: ... The Renilla luciferase CDS was amplified from a pRL-null vector (Promega) by PCR using PrimeSTAR GXL DNA Polymerase (TaKaRa ...
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bioRxiv - Cancer Biology 2021Quote: ... and 3 ng of the Renilla Luciferase expressing pRL-SV40 plasmid (Promega) for normalization ...
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bioRxiv - Microbiology 2021Quote: ... The cells were transfected with pISRE-TA-Luc and pRL-TK (Promega). After 24 h ...
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bioRxiv - Developmental Biology 2022Quote: ... or pRL-TK and pGL3-BARL (β-catenin activated reporter luciferase, Promega) or pRL-TK and pGL3-8xGli (a gift from Philip Beachy ...
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bioRxiv - Immunology 2021Quote: ... 4D6 cells were co-transfected with a renilla control plasmid (pRL Promega), and a luciferase reporter plasmid (pGL4.10[Luc2] ...
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bioRxiv - Cancer Biology 2023Quote: pRL-TK-renilla and pGL3 plasmids were obtained from Promega (Charbonnieres, France). pEF-cmyc-RIP140 and pEGFP-RIP140 have been previously described (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... TK-minimal promoter was excised from pRL-TK (Promega, Madison, Wisconsin, USA) using BglII/HindIII restriction enzymes and instered into corresponding sites of pGL4.12 (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... China) and pRL-SV40 (Renilla luciferase, obtained from Promega, Catalog No. E2231). All plasmids were DNA sequence verified.
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bioRxiv - Cancer Biology 2023Quote: ... Melanoma cells were transfected with combinations of reporter constructs: pRL-CMV (Promega) and either pCDH-Cuo-IRES-RFP or pCDH-Cuo-hMITF-M-IRES-RFP ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Transient transfection of the dual NanoLuc and Halo-Tagged tagged BRD4Tandem plasmid (Promega) was performed as described previously52 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 50 ng of plasmids encoding the HiBiT-tagged proteins using FuGENE HD (Promega). Cells were trypsinized (Gibco ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Genetics 2019Quote: ... A cDNA encoding Myc epitope-tagged human TRAPα was synthesized by Integrated DNA Technologies (IDT) and was subcloned into the pTarget mammalian expressing vector (Promega).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were co-transfected with pcDNA5/FRT construct encoding haemagglutinin (HA)-tagged human CB1 receptor cDNA and pOG44 (Flp recombinase plasmid) using transfection reagent Fugene HD (Promega) as previously described for AtT-20 pituitary tumour cells [26] ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... internal control plasmid pRL-TK (0.02 μg/well; Promega Corp.; Fitchburg, WI, USA), and the indicated expression constructs ...
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bioRxiv - Microbiology 2021Quote: ... was acquired from Agilent Technologies. The pRL-TK Renilla Luciferase vector (Cat. # AF025846) was purchased from Promega.
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bioRxiv - Microbiology 2021Quote: ... ISRE-firefly luciferase and pTK-renilla luciferase (pRL-TK) plasmids were from Promega. Vectors expressing MAVS ...
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bioRxiv - Cell Biology 2020Quote: ... Fluc reporter plasmids and pRL-TK renilla luciferase (rluc) transfection control plasmid (Promega) were co-transfected into these cells using jetPRIME® transfection reagent (Illkirch-Graffenstaden ...
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bioRxiv - Developmental Biology 2019Quote: ... the reporter vectors were co-transfected into HEK293T cells with pRL-TK (Promega) and Etv5 over-expression plasmid pTrip-CAGG-Etv5 using Dual Luciferase Reporter Gene Assay Kit (Beyotime) ...
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bioRxiv - Genomics 2020Quote: ... 0.02 ug of the pRL-CMV Renilla Luciferase plasmid (Promega Accession No. AF025843) was co-transfected with firefly luciferase plasmids ...
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bioRxiv - Genomics 2022Quote: ... as well as the WT pGL3-Basic vector and pRL-TK (Promega, E2241), were midi-prepped (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... as well as the WT pGL3-Basic vector and pRL-TK (Promega, E2241), were midi-prepped (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The pRL-TK Renilla luciferase reporter plasmid was purchased from Promega (Madison, WI). E ...
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bioRxiv - Developmental Biology 2023Quote: ... pCDNA3 HSV-TK 2xMyc Su(H)VP16 and pRL TK (Promega, Madison, USA). For the Luciferase assay ...
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bioRxiv - Cell Biology 2024Quote: ... the TK-minimal promoter was removed from pRL-TK (Promega, Madison, Wisconsin, USA) and inserted into the appropriate locations of pGL4.12 (Promega ...
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bioRxiv - Systems Biology 2023Quote: ... The biotinylated proteins bound to the beads were digested with sequencing-grade trypsin (Promega, Madison, WI, USA) in 100 mM ammonium bicarbonate (pH 8 ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Systems Biology 2019Quote: Cells were lysed and recombinant proteins were isolated using Magne® HaloTag® Beads (Promega) as previously described (15) ...
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bioRxiv - Developmental Biology 2020Quote: ... internal control pRL/TK-luciferase reporter and the dual luciferase reporter assay kit (Promega). Neural stem / progenitor cells were seeded in coated 24-well plates (1.6×105 cells per well ...