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Citations for Promega :
251 - 300 of 1739 citations for Recombinant Human PRL Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Biochemistry 2021Quote: ... yeast (Saccharomyces cerevisiae) cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc (WI ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... C-terminal large bit (LgBiT) tagged construct (Promega) which is transcribed under control of the HSV-TK promoter.
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bioRxiv - Molecular Biology 2023Quote: ... N-terminal small bit (SmBiT) tagged construct (Promega), with the native HSV-TK promoter swapped out for a CMV promoter ...
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bioRxiv - Cell Biology 2023Quote: ... the HaloTag-tagged SUV420H2 expression vector (FHC26822; Promega) was used as a template for PCR amplification of the target regions ...
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bioRxiv - Cell Biology 2023Quote: The HaloTag-tagged KDM4D expression vector (FHC06842; Promega), the PB533-based H2B-Halo expression vector (Uchino et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed and translated FLAG-tagged ERRα protein was made using the TnT reticulocyte reaction (Promega cat #L1170, 2h on 37°C) or the TnT wheat germ extract (cat #L5030 ...
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bioRxiv - Genomics 2019Quote: ... and Daudi cells were nucleofected with 0.9 μg of pGL3-Promoter vector along with 0.1 μg of pRL-TK Renilla luciferase vector (Promega) in 16 well strips in a 4D nucleofector with the following protocols and buffers in 20 μL of total volume ...
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bioRxiv - Cancer Biology 2022Quote: ... Renilla luciferase plasmid (pRL-SV40) and Control luciferase plasmid (pGL3) were obtained from Promega (#E2231 and #E1751, respectively). 48-hr post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 cells were co-transfected with the firefly reporter vectors and the Renilla luciferase plasmid pRL-TK (Promega) as an internal control ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected the next day with a mixture of TP1-firefly luciferase and pRL-TK (Promega) plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... HepG2 cells were seeded in 24-well plates at a density of 100,000 cells/well and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 3000 reagent ...
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bioRxiv - Synthetic Biology 2024Quote: ... Firefly Luciferase Arbitrary Units (Luciferase [A.U.]) were calculated by normalizing each sample’s Firefly Luciferase activity to the constitutive Renilla (pRL-TK, Promega) activity detected in the same sample ...
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bioRxiv - Developmental Biology 2023Quote: ... The Carm1-reporter or its mutated reporters was co-transfected into 293T with the pRL-TK plasmid (Promega) expressing renilla for normalization ...
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bioRxiv - Neuroscience 2020Quote: ... 1U/uL recombinant RNAsin(Promega), 10mM Na3VO4 ...
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bioRxiv - Immunology 2021Quote: ... Recombinant RNasin Ribonuclease inhibitor (Promega), and SuperScript II reverse transcriptase (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... and recombinant RNasin inhibitor (Promega). The transcripts for genes of interest were measured by real-time qPCR with a Lightcycler 480 II system (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... and ribonuclease (RNasin Recombinant, Promega) at concentrations of 1:1000 (sucrose buffer ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated lysine residues were added to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA complex (Transcend tRNA; Promega) and incorporated into nascent proteins during translation ...
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bioRxiv - Cell Biology 2021Quote: ... with two plasmids – one containing the pRL Renilla luciferase gene under the control of the CMV promoter (# E2261, Promega) and the other containing the firefly luciferase gene under the control of the Pomc promoter (−646bp to +65bp ...
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bioRxiv - Cell Biology 2021Quote: Cos7 cells were transfected with Nanog promoter-luc or Cyclin A2 promoter-luc along with pRL-SV40 (Promega, USA), Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences ...
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bioRxiv - Developmental Biology 2020Quote: ... with 100 ng of firefly luciferase reporter p12xAT_luc or p12xAT_luc(Mut) (Hojo et al., 2016) 15 ng of the pRL-TK Renilla luciferase control plasmid (Promega) and 100ng (unless otherwise indicated ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with 800 ng of pGL3 constructs and 20 ng of pRL-TK renilla reporter vector by Transfast transfection reagent according to manufacturer’s protocol (Promega). 24 h after transfection ...
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bioRxiv - Zoology 2020Quote: ... K562 cells were transfected with 0.1 μg of Renilla luciferase expression plasmid pRL-TK (internal control for normalizing transfection efficiency; Promega) and 0.4 μg of abcb4 promoter construct ...
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bioRxiv - Genomics 2020Quote: ... Each well was transfected with 25ng of the corresponding putative enhancer-cloned pGL3-promoter luciferase reporter along with 1.5ng Renilla (pRL-TK Vector, Promega) control reporter vector using FuGENE HD transfection reagent as previously described [20] ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with 800 ng of pGL3 constructs and 20 ng of pRL-TK renilla reporter vector by Transfast transfection reagent according to manufacturer’s protocol (Promega) and after 48h cells were lysed ...
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bioRxiv - Genomics 2022Quote: MM001 and MM047 were seeded in 24-well plates and transfected with 400 ng pGL4.23-enhancer vector + 40 ng pRL-TK renilla vector (Promega) with Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... All enhancer or mutant enhancer reporter constructs (200ng) were transfected with 20ng of pRL (Renilla luciferase control vector, Promega) and either empty vector (pcDNA3.1 ...
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bioRxiv - Microbiology 2022Quote: ... either with SARS-CoV-2 S and Jun or with ACE2 and Fos plus the pRL Renilla Luciferase (Promega) to normalize the signal ...
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bioRxiv - Evolutionary Biology 2022Quote: ... were transfected with 100ng of the TE containing luciferase plasmid and 10ng of the pRL-null renilla vector (Promega) with 0.1μl PLUS reagent (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... The pGL4.23 constructs were co-transfected with a renilla internal control vector pRL-TK (Promega, Madison, Wisconsin, United States) in a 50:1 ratio in SH-SY5Y cells in 6-well plates with a seeding density of 1 million cells per well ...
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bioRxiv - Microbiology 2023Quote: ... Other plasmids used in this study include the pEGFP-N1 (Clonetech) and pRL-CMV vector (E226A) acquired from Promega. Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 105 HEK293T cells were seeded into 24-well plates and co-transfected with pGL4.10-based plasmids and pRL-SV40 Renilla luciferase plasmid (Promega). After 48 hours ...
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bioRxiv - Cell Biology 2023Quote: ... we co-transfected 100 ng of pGL4-Dusp13 or pGL4-Dusp27 with 50 ng of pRL-TK vectors (Promega) and varying amounts (0 ...
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bioRxiv - Immunology 2023Quote: ... Renilla luciferase internal control vector pRL-TK and the dual luciferase reporter assay kit were from Promega (Madison, WI). Phusion PCR enzyme ...
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bioRxiv - Biophysics 2022Quote: ... Biotinylated tubulin was then protease digested by AspN (Promega) at 37 °C overnight (1:50 w/w ratio) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... were attached to biotinylated magnetic beads (Promega, Southampton, UK) were washed in 100μl 6x SSC ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNAs were captured using streptavidin magnetic beads (Promega), washed with salt buffers ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.625 μl of recombinant RNasin (Promega), 1 μl of random hexamer primers (Promega ...
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bioRxiv - Physiology 2019Quote: ... 100 U/mL recombinant RNasin (Promega), 100 μg/mL cycloheximide ...
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bioRxiv - Cell Biology 2022Quote: ... 0.2U/μL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Microbiology 2020Quote: ... Cell fusion activity was quantitatively determined after 24 h by measuring firefly luciferase activity expressed by pT7EMCVLuc and was standardized with Rluc activity expressed by pRL-CMV by using the Dual-Glo luciferase assay (Promega) (27 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were co-transfected with 200 ng of pANF638L vector and 20 ng of pRL-TK (a Renilla luciferase control vector; Promega) in 2.4 μL FuGENE HD (Promega ...
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bioRxiv - Genomics 2020Quote: ... Cells were co-transfected with MMTV LTR-Firefly Luciferase (pAGMMTVLu, gift from Laboratory of Patricia Elizalde) and CMV-Renilla luciferase (pRL-CMV, Promega) plasmids using lipofectamine plus 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Molecular Biology 2021Quote: ... NM_001016) and SLC25A6 (accession number: NM_001636) were custom synthesized (IDT) and cloned into pRL-CMV Renilla luciferase vector (Promega, E2261) using oligos in Supplementary file 1 ...
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bioRxiv - Plant Biology 2019Quote: ... the coding sequences of bes1-D(L) from cDNA of the bes1-D mutant and RLUC from the vector pRL vector (Promega) were amplified by PCR using the following primers: