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Citations for Promega :
2801 - 2850 of 5281 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The size of any positive IS2404 detection was assessed against 2 µL of 100 bp DNA Ladder (Promega) and run at 50 V for 1.5 hrs before being visualised with an EZ Gel Documentation System (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis and qPCR were performed using the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of Turbo DNase treated total RNA was incubated with or without 25 U RNase One (Promega) and 25 μg RNaseA (Thermo Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... a 27-nucleotide spacer sequence was inserted between the BbsI and ClaI restriction sites of psiCHECK-2 (Promega) to remove the firefly luciferase gene sequence and its promoter sequence ...
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bioRxiv - Cell Biology 2024Quote: ... 20 μl of CellTiter- Glo 2 reagent was added to each well according to the manufacturer’s protocol (Promega).
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bioRxiv - Genetics 2024Quote: ... 2 µl of the extracted genome solution was used as a template for Genome PCR by GoTaq (Promega). The sequences of gw182 gene specific primer set designed around the CRISPR target site were 5’ -GAG TCC AAT TTG AGA AAC GGA GGT CA-3’ and 5’ - CTG ATC GTT TGC GCT TAA CTT CAT TAA TTC T-3’ ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were further diluted to 2 M urea with 100 mM TEAB and trypsin (Trypsin Glod, Promega) was added at a ratio of 1:50 and the samples were incubated at 37°C over night ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 75 μL (1:1 volume) of One-Glo™ reagent (E6110, Promega) was added to each well ...
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bioRxiv - Neuroscience 2021Quote: ... IΒΑ1 (1:250, rabbit, Wako, 01919741) and p75NTR (1:1000, rabbit, G3231, Promega). Sections were washed 3×20 min in PBS prior to incubation with secondary antibodies (Alexa Fluor ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were collected and mixed 1:1 with BrightGlo Luciferase assay reagent (Promega) and luminescence was measured using a luminometer (Molecular Devices ...
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bioRxiv - Molecular Biology 2022Quote: ... alongside a 1:1 molar ratio of Luciferase (pGL4.23) to Renilla (pGL4.75, Promega) vectors ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then diluted 1:1 with Milli-Q water and trypsin (Promega) added at the same enzyme/protein ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM DTT) or with 1 unit of RQ1 DNase (Promega; RNase-free) in 40 μL of RQ1 buffer (40 mM Tris-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: First strand synthesis was performed in 25 μl reaction volumes using 5 μl of MMLV-RT 5X buffer (Promega, Wisconsin, UK), 1.25 μl of dNTPs (10mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Microbiology 2019Quote: ... proteins were firstly digested for 5 h at 37°C with 250 ng rLys-C Mass Spec Grade (Promega, Madison, USA). Samples were then diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration of 2M urea and then re-incubated overnight at 37°C with 1 µg Sequencing Grade Modified Trypsin (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... 25uL of reconstituted CellTiter-Glo Luminescent Cell Viability reagent was added to each well and incubated for 5 minutes (Promega, G7571). Luminescence readings were taken using the BioTek Synergy H1 Hybrid Reader ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... The gel particles were eventually rehydrated on ice in a solution containing 5 ng/μL of trypsin (Promega, modified sequencing grade) in 50 mM NH4HCO3 ...
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bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
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bioRxiv - Plant Biology 2020Quote: ... Individual reactions conducted with UGA-CPES consisted of a 10 µL mixture containing 5 µL of GoTaq Green Master Mix 2X (Promega Corporation), 0.5 µL of template DNA ...
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bioRxiv - Microbiology 2021Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2 h at 37° C with 5% CO2 ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Microbiology 2021Quote: ... an 150 µL aliquot of the protein sample was digested with 5 µg of sequencing grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Microbiology 2020Quote: ... Amplifications were performed in a 50 µl final volume using 5 µl of DNA template and 2.5 units of GoTaq polymerase (Promega, Madison, WI). Final concentrations of MgCl2 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... miR159 and U6 RNA (see Table S3) were 5’ end-labelled with [γ-32P]ATP using T4 polynucleotide kinase (PNK) (Promega).
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Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
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bioRxiv - Synthetic Biology 2021Quote: ... Media was again removed and replenished with 30 mL phenol-red free Opti-MEM supplemented with 5 mM CT-TAMRA (Promega, G8251), except for no CT-TAMRA control wells ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were vigorously shaken at 80 rpm for 5 min prior to adding 75 µl of CellTiter-Glo 3D Reagent (Promega #G968B) to each well ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was diluted 5 folds with distilled water and used in a reaction mix composed of 1x GoTaq qRT-PCR master mix (Promega, USA) and 10 nM primers for RT-qPCR ...
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bioRxiv - Plant Biology 2020Quote: ... All PCR amplifications were assembled on ice in 25 μl reactions using 5 μl of 5X Green GoTaq buffer (Promega M3001), 0.5-1 μl of each primer (10 μM) ...
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bioRxiv - Microbiology 2022Quote: ... the PCR was performed in a total volume of 25 μl containing 5 X GoTaq® buffer (Promega, Madison, WI, USA), 2.5 mM MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was washed three times for ten minutes and then incubated for 5 minutes with an enhanced chemiluminescence reagent (Promega W1001). The blot was imaged in an Azure Biosystems C600 imaging system ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Biophysics 2023Quote: DNA dT70 was diluted to ∼100 pM in buffer (50 mM Tris-acetate pH 7.7, 5 mM Mg-acetate, 50 mM Na-acetate) and SSB (Promega Corporation, USA) was supplemented in specified concentrations (Tab ...
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bioRxiv - Microbiology 2023Quote: ... The migrated cells in the lower chamber were stained with 5 μl of CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega # G3580) for 2.5 h at 37° C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.