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Citations for Promega :
2601 - 2650 of 5281 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of template DNA was added to 5 μl Green GoTaq Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 1.25 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Pathology 2022Quote: ... G490 5 × All-In-One RT MasterMix (abm) was used for reverse transcription according to the manufacturer’s protocol (Promega). The qRT-PCR analysis was performed in 96-well plates using the BIO-RAD CFX96 detection system ...
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bioRxiv - Immunology 2022Quote: ... Transduced cells were incubated for 24 hours at 37°C/5% CO2 and luminescence measured by addition of 40μl of ONE-Glo reagent (Promega) with detection using a Tecan Spark plate reader ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplifications were carried out in a total reaction volume of 10μL containing 5 μL of 2xGoTaq master mix (Promega) and 2.5 μL of 4M betaine ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were precipitated overnight with 5 volumes of cold 0.1 M ammonium acetate in 100% methanol and digested with sequencing-grade trypsin (Promega) and each sample was analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (40Ul/µl stock, 5 ml/ml of buffer, Promega). We rinsed sections ...
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bioRxiv - Microbiology 2021Quote: ... seriolae gDNA was extracted from 5-day old cultures using the Wizard® Genomic DNA Purification Kit (Promega, USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK cells were transfected with 5’UTR-reporters for 24h using ViaFect reagent according to the manufacturer’s protocol (Promega). Next ...
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bioRxiv - Immunology 2021Quote: ... Luminescence was measured after 20 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).
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bioRxiv - Immunology 2021Quote: ... Luminescence was measured after 20 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).
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bioRxiv - Immunology 2022Quote: ... Luminescence was measured after 22 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... a multiplexed caspase/viability assay was performed as described before [5] using the Multiplex Assay ApoLive-Glo (Promega, G6411) kit.
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bioRxiv - Molecular Biology 2024Quote: ... 400 μl cold cell lysis buffer (20 mM HEPES, pH 7.4, 100 mM KCl, 5 mM, MgCl2, 500U/ml RNasin-Plus (Promega), 1x protease inhibitor cocktail (200X ...
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bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
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bioRxiv - Systems Biology 2023Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Cell Biology 2023Quote: ... About 5 µg of the purified mRNA was used to generate cDNA using the Go script kit (Promega #A5001). The KRAS coding region was amplified using a pair of primers Kras_Exon1-F (5’ CCGCCATTTCGGACTGGGAGCGAGCGC 3’ ...
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bioRxiv - Immunology 2023Quote: ... Luminescence was measured after 22 hours of incubation at 37°C with 5% CO2 with a luminometer using the Bio-Glo-TM Luciferase Assay Reagent according to the manufacturer’s instructions (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... DNA from My-La cells was amplified by use of primers GATA3_AICE_KpnI s 5’-GCGGTACCATACAGACCCTTCCAGCCAC and GATA3_AICE_XhoI as 5’-GCCTCGAGAACAGATGTGGGGAGTCAGA and cloned via KpnI and XhoI into the multiple cloning site (MCS) of pGL3 (Promega). All constructs were verified by sequencing.
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bioRxiv - Immunology 2023Quote: Promoter constructs were created by cloning the immediate 4.5-kb region adjacent to the 5’ TSS of SPINK7 into the promoterless Nano-luciferase reporter vector pNL1.1-NL (Promega). The 4.5-kb sequence and subsequent constructs were created by using primers with the restriction enzyme sites KpnI-HF and XhoI ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5 µL of the supernatant were diluted in 45 µL of Luciferase Assay Reagent (Luciferase Assay System, E1500, Promega) and measured at a Synergy HT reader (BioTek ...
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bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using primers listed in Supplementary Table 5 and products cloned using the pGEM-T Easy Vector System I (Promega) or Phusion HF DNA polymerase (NEB ...
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bioRxiv - Biophysics 2024Quote: ... The next day cells were treated with compounds and assessed for cell growth 5 days later using CellTiterGlo (Promega).
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bioRxiv - Cell Biology 2024Quote: ... 5 regions of ieCTNNB1 and the region containing the mutation site were respectively cloned into pGL3- promoter vector (Promega). HEK293T ...
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bioRxiv - Systems Biology 2024Quote: ... Proteins were digested with 5 µL of 100 mM Ambic pH 8.8 that contained ∼250 ng/µL of Trypsin/rLysC enzyme mix (Promega) (Total amount 1.25 µg ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Thawed tissue was minced and incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, Madison, WI)) for 5 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then co-transfected with either 0.3 μg/well of Smad4 firefly luciferase reporter plasmid constructs (pLuc366 or pLuc207) or the control pGL3-Basic vector (Promega, San Luis Obispo, USA). The renilla luciferase plasmid was also co-transfected to correct for variations in transfection efficiency (45 ng/well) ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transfected with 1 μg of DNA (1 μg of an L1-expressing plasmid or 0.5 μg of the L1-expressing plasmid and 0.5 μg of either a pCMV-3Tag-8-Barr control or ISG-expressing plasmid) using 3 µL of FuGENE HD transfection reagent (Promega, Madison, WI, United States) and 100 µL of Opti-MEM (Gibco ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... Media was changed at 3 hpi and luciferase luminescence was measured at 24 hpi using the Nano-Glo® Luciferase Assay (Promega, Madison, USA, #N1130) kit ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Plant Biology 2020Quote: ... genomic DNA was extracted from 2-wk old seedlings using the Wizard Genomic DNA purification kit (Promega) and was subjected to PCR analysis using two AtNIP2;1 gene specific primers and the left border T-DNA primer (all primers used in this study are described in Supplemental Table 1) ...
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bioRxiv - Plant Biology 2021Quote: ... Selection was done twice for 2 weeks on medium containing 25 µg/ml G418 (Promega, Mannheim, Germany) starting 2 weeks after transformation with a 2-weeks release period in between ...
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bioRxiv - Developmental Biology 2022Quote: ... The results were calculated using the 2-ΔΔCT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or with 0.05 µg GLP-1R-SmBiT and 0.05 µg LgBit-β-arrestin-2 (Promega, Hampshire, UK) plus 0.9 µg pcDNA3.1 for 24 hours ...
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bioRxiv - Molecular Biology 2019Quote: The entire genome was amplified by PCR using GoTaq 2× Hot Start Colorless Master Mix (Promega, USA) using overlapping primer sets (Ali et al. ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Biophysics 2021Quote: ... Constructs (2 μg) were transfected into HEK293 cells on glass coverslips using Fugene HD (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
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bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
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bioRxiv - Molecular Biology 2022Quote: Cell proliferation was measured in BAG-1 CRISPR knockout cells or siRNA treated cell in response to vehicle (0.1% DMSO) and Thio-2 (5μM and 50μM) using CellTiter-Glo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM TCEP) was added to the sample followed by 40 µg of sequencing grade trypsin (Promega). Proteins were digested overnight rotating at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... measured using a Biotek Synergy 2 multimode plate reader and the BacTitr-Glo Cell Viability assay (Promega). There was a linear relationship between concentration and luminescence (R2 =0.998) ...
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bioRxiv - Immunology 2023Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg sequencing-grade trypsin (Promega) at 37°C overnight ...