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Citations for Promega :
2651 - 2700 of 5281 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 20 μl of 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) (AqueousOne, Promega) at 37°C for 1-2 hours for MTS colorimetric cell viability assay ...
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bioRxiv - Biochemistry 2024Quote: ... Each sample was divided into 2 aliquots and digested with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) and AspN (Sequencing Grade ...
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bioRxiv - Cancer Biology 2024Quote: ... Urea was diluted to working 2 M concentration with 20 mM HEPES (pH 8.0) and tryptic (Promega) digestion performed at an enzyme to substrate ratio of 1:25 overnight with incubation at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were incubated for 2 h at 37 °C followed by DNase RQ1 (Promega, Madison, WI) treatment (2 Units ...
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bioRxiv - Microbiology 2024Quote: ... ATP content was measured using the BacTiter-Glo™ Microbial Cell Viability Assay Kit (Promega, Cat.G8230/2). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were mixed in a 1:1 ratio with substrate (Promega; E1531) and luminescence was measured ...
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bioRxiv - Microbiology 2021Quote: ... the samples were diluted 1:100 in 1 × passive lysis buffer (Promega) and 5 µl were transferred into a white Nunc 96-well plate ...
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bioRxiv - Genomics 2023Quote: ... Samples were then diluted 1:1 with MilliQ water and trypsin (Promega) added at the same enzyme to protein ratio ...
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bioRxiv - Immunology 2023Quote: ... and 1 μL RQ1 DNase (1 U/μL, Promega, cat. no: M6101) and then incubated at 37 °C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl forward primer and 1 µl reverse primer (Promega corporation, USA), 3 µl extracted DNA and 14 µl nuclease free water and initial denaturation at 95°C for 10 minutes followed by 35 cycles including annealing temperature which varied in different reaction ...
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bioRxiv - Microbiology 2020Quote: ... 1 U RNasin (Promega) and 1 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:1,000 RNasin (Promega)) using a 2ml Dounce homogeniser ...
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bioRxiv - Neuroscience 2020Quote: ... furimazine (Promega, 1:2000), 20 mM HEPES ...
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bioRxiv - Neuroscience 2019Quote: ... RNasin (Promega, 1:1000) using a 2 mL glass-Teflon homogenizer with 12 gentle strokes ...
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bioRxiv - Neuroscience 2019Quote: ... 1× Protease Inhibitor (Promega), Hoechst 33342 10 ng mL−1 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1× Protease inhibitor (Promega), 0.1 mM DTT (Thermo Fisher)] per 100 mg of tissue with 15 strokes of the loose and 15–20 strokes of the tight pestle ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µl DTT (Promega), in 20 µl Superscript II buffer (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μg trypsin (Promega) was added to the S-trap for 90 minutes at 47 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... or (1:25,000) (ProMega) were used ...
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bioRxiv - Neuroscience 2022Quote: ... and 1 % RNasin (Promega), and incubated for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Luciferase (1:2500, Promega), Myc tag (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 mM luciferin (Promega), 14.5 mM NaHCO3 (Sigma) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1Uμl-1 RNasein (Promega), 0.1% IGEPAL (Sigma)) ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.5ml 1% digitonin (Promega), 0.5ml 10% Tween-20 (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2023Quote: ... 1 x buffer (Promega), 2.5 mM MgCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... 1:10000 (Promega: W4021) and in 3.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and further incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... diluted 1:5000 in 5% milk in TBST were used for detection by the addition of an enhanced chemiluminescence (ECL) substrate (Promega). Western blot quantification was performed using Fiji software (Schindelin et al. ...
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bioRxiv - Neuroscience 2021Quote: IP and pull-down probes of DG NSCs were subjected to on-bead digestion (Hubner et al., 2010) by trypsin (5 µg/ml, Promega) in 1.6 M Urea / 0.1 M Ammonium bicarbonate buffer at 27 C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 40% confluent HAP 1 WT cells (seeded in a 10-cm dish) were co-transfected with the two plasmids described above (5 µg each) using 30 µl Fugene6 transfection reagent (Promega) in OptiMEM medium ...
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bioRxiv - Cancer Biology 2019Quote: ... 5% CO2) with a final DMSO concentration of 0.3% and assayed for viability using the CellTiter-Glo Reagent (Promega Corporation) as described in manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Expression of RLuc fusion proteins was estimated by measuring the luminescence of the cells after incubation with 5 µM coelenterazine H (Promega). In parallel ...
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bioRxiv - Cell Biology 2020Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) at room temperature for 90 min ...
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bioRxiv - Bioengineering 2021Quote: ... Ligations of the digested plasmid backbones and PCR products occurred for 5-10 minutes at RT using T4 DNA ligase (Promega) prior to transformation into NEB® Turbo Competent E ...
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bioRxiv - Biochemistry 2021Quote: The samples were reduced and digested in 25 μL co-IP digest buffer (50 mM Tris-HCl, 5 ng/μL trypsin (sequencing grade, modified, Promega), 2 M urea ...
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bioRxiv - Cancer Biology 2020Quote: ... the blot was blocked with either 5% nonfat-milk or 5% BSA in TBST before addition of primary antibodies and followed with peroxidase-conjugated secondary antibody (Promega). Protein bands were detected using SuperSignal Chemiluminescent Substrate (Pierce ...
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times in TBST for 5 minutes and were then incubated with HRP-conjugated secondary anti-mouse-IgG-H&L chain (Promega) or anti-rabbit-IgG-F(ab’)2 (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... Halo fusion proteins were produced from 1.5 µg plasmid DNA in a 15 µL reaction using TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega). Protein expression was confirmed by Western blot with Anti-HaloTag monoclonal Ab (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... The samples were sonicated for 30 seconds before centrifugation and 5 μl of the supernatants were collected for luciferase expression reading by the dual-luciferase reporter assay system (Promega) using a GLOMAX 20/20 luminometer (Promega) ...
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bioRxiv - Immunology 2021Quote: ... cells13 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody–bead mixture was resuspended in 500 μl of the reaction mixture containing 10 μg of fragmented total RNA,100 μl of reaction buffer and 5 μl of RNasin Plus RNase Inhibitor (Promega), and incubated for at least 4 h at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... Proteins were digested to peptides by addition of 25 μl of 50 mM ammonium bicarbonate containing 5 μg of trypsin (Promega) and incubating (37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 150 ng trypsin (Trypsin Gold, Promega) for 90 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... Remaining iodoacetamide was quenched by adding 5 mM DTT and the proteins were digested with 200 ng trypsin (Trypsin Gold, Promega) at 37 °C ON ...
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bioRxiv - Biochemistry 2022Quote: ... which had been pre-incubated in 200 μL Ex-Cell 420 medium plus 5 μL FuGENE HD transfection reagent (Promega) for 20 minutes at 27 °C ...
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bioRxiv - Immunology 2022Quote: ... cells9 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2022Quote: ... for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...