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Citations for Promega :
201 - 250 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Half of the virus containing media was aspirated and replaced with luciferase substrate (Bright-glo luciferase assay system, Promega) and incubated for 5 minutes at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out with oligo(dT) primers using Moloney murine leukemia virus reverse transcriptase according to the manufacturer’s instructions (Promega). The qPCR reaction was performed on an Applied Biosystems QuantStudio3 thermocycler using a SYBR™ Green PCR Master Mix (Thermo Fisher scientific) ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized using 1μg total RNA with 200 units moloney murine leukemia virus reverse transcriptase (Promega, Southampton UK), primed with 2μM oligo dT (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Immunology 2023Quote: ... RNA was reverse-transcribed into cDNA using Maloney murine leukemia virus reverse transcriptase Taq polymerase (Promega, Madison, WI, USA). For quantitative RT-PCR ...
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bioRxiv - Developmental Biology 2021Quote: PCR (RT-PCR) was conducted using GoTaq DNA polymerase (Promega). PCR programs were the following ...
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table 3.
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bioRxiv - Microbiology 2021Quote: ... Colony PCR was performed using PCR 2x Master Mix (Promega) with primers listed in Table S3.
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bioRxiv - Cell Biology 2023Quote: ... PCR reactions were prepared with GoTaq PCR Master Mix (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... q-PCR was performed using GoTaq q- PCR Mastermix (Promega) according to manufacturer’s instructions on a Rotor-Gene Q instrument (Qiagen) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR buffer (Promega) and MgCl2 (2.5 mM) ...
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bioRxiv - Microbiology 2023Quote: ... PCR cleanup (Promega), and quantification ...
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bioRxiv - Genetics 2024Quote: ... PCR (Promega M7801) genotyping was performed by amplifying 5’ and 3’ junctions ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was synthesized by in vitro transcription of the PCR products using the T7 RNA polymerase (Promega kit). Then ...
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bioRxiv - Cell Biology 2020Quote: ... and gel extractions were performed with the Wizard SV Gel and PCR clean-up kit (Promega, Madison, USA). Sequencing of plasmids was performed at the Massey Genome Centre ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were inserted into the pGEM-T easy vector kit as described by the manufacturer (Promega, USA). Insert were then PCR-amplified and the resulting PCR products were sequenced.
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bioRxiv - Molecular Biology 2023Quote: ... The resulting templates were purified using the Wizard® SV Gel and PCR Clean-Up System kits (Promega) and transcribed using the Megascript T7 kit (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR mixtures were prepared as recommended by the GoTaq Flexi DNA polymerase kit protocol (Promega, Madison, Wisconsin, USA). The following temperature programme was used ...
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bioRxiv - Microbiology 2022Quote: ... PCR was conducted in Promega PCR master mix (Promega, Madison, WI). SYBR green-based qPCR and probe-based qPCR was conducted in SsoAdvanced universal SYBR green supermix (Bio-Rad Canada ...
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bioRxiv - Zoology 2024Quote: RT-PCR was performed using AccessQuick RT-PCR System (Promega, France) in total reaction volume of 25 µl with 5 µl of RNA extracts and 400 nM of forward primer (5’-ATTCGGGAGGAGACGTGGTA-3’ ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed using GoTaq® Long PCR Master mix (Promega) and each reaction consisted of ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA (50-250 ng) was reverse-transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega, M368B) and amplified using the iTaqTM Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... Reverse transcription was achieved with 4 µg of RNAs in the presence of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (RNase H minus, Point Mutant, Promega) after annealing with an anchored oligo(dT)18 primer as described (Wirth et al. ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus Reverse Transcriptase (Promega). RT-qPCR was performed on the ViiA 7 Real-Time PCR System using GoTaq qPCR master mix (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... Resulting ribosomal complexes were analyzed by toe-printing as described (Pisarev et al., 2007) using avian myeloblastosis virus reverse transcriptase (AMV RT) (Promega) and [32P]-labeled oligonucleotide primers complementary to HalV nt ...
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bioRxiv - Cell Biology 2022Quote: ... synthesis was done by using 1 μg RNA as template and the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (Promega), in 20 μl reaction volume according to the instructions manual ...
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bioRxiv - Genetics 2021Quote: ... Around 1 μg total RNA was used to synthesize cDNA by using oligo-dT18 primer and Moloney murine leukemia virus reverse transcriptase (M1701, Promega). The RT-qPCR was performed using iTaq Universal SYBR Green Supermix (172-5125 ...
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bioRxiv - Plant Biology 2021Quote: ... reverse transcribed with oligo(dT)15 in a 20-μL reaction mixture using the Moloney murine leukemia virus reverse transcriptase (Promega) or the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of culture supernatants were collected every 6 hours and nanoluciferase activities associated with released virus in the supernatants were measured using Nano-Glo luciferase assay system (Promega).
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated for three days before assessing viability and virus-induced cell death by CellTiter Glo according to manufacturer protocol (Promega). Luminescence was quantified using a plate reader (Cytation 5 ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was used for first-strand cDNA synthesis using Moloney murine leukemia virus (MMLV) reverse transcriptase enzyme (Promega, USA) and random primers (Biodynamics ...
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bioRxiv - Physiology 2022Quote: ... and 0.5 μg of reference plasmid pRL-TK carrying the Renilla luciferase gene under the control of the simian virus 40 enhancer and promoter (Promega). Lipofectamine 3000 reagent (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: Cells infected with a luciferase-encoding virus were lysed 2 days after infection with a Bright-Glo Luciferase Assay System (E2620, Promega) and the luminescent signal was measured using a GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNAs were synthesized according to Daniel-Vedele and Caboche (1993) using Moloney murine leukemia virus reverse transcriptase and oligo(dT)15 primers (Promega) or using a SuperScript III Fast strand synthesis system (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Diluted patient sera was mixed with 25μL of pseudovirus at a titer such that virus-only wells would achieve a luminescence of approximately 100,000 RLU on Promega Glomax plate reader (Promega; GM3000). Virus-serum mixtures were incubated for 1hr at 37C ...
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bioRxiv - Microbiology 2022Quote: PCR amplicons were gel extracted using the QIAquick gel extraction kit and ligated into pGEM-T Easy plasmid (Promega). Vectors containing the PCR products were then cloned into chemically competent E ...
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bioRxiv - Molecular Biology 2024Quote: ... The linearized plasmid was then purified using the Wizard® SV gel and PCR clean-up system kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: cDNA levels were measured by quantitative PCR using the GoTaq® qPCR kit (Promega Ref A6002, Madison, WI, USA) according to the manufacturer’s instructions under standard cycling conditions ...
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bioRxiv - Biochemistry 2024Quote: ... followed by gel purification of the products with a Wizard SV Gel and PCR Clean-Up System kit (Promega). Transcription products were separated on a 6% denaturing polyacrylamide gel (AccuGel 19:1 ...
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bioRxiv - Biochemistry 2020Quote: ... The PCR reaction was subjected to DpnI digestion and PCR cleanup (Promega Wizard SV Gel and PCR Clean-Up System). 30 ng of PCR amplified pUC19 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were carried out using GoTaq green PCR reaction buffer (Promega). DNA was cycled according to the following protocol ...