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Citations for Promega :
151 - 200 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701, Promega) for 90 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Cell Biology 2023Quote: ... A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 µl RT buffer 5x (Promega) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested by DNase I before reverse transcription with murine leukemia virus reverse transcriptase using random primers (Promega). qPCR was performed with and a CFx96 Touch Real-Time PCR system (Bio-rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA samples were converted to cDNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase enzyme (Promega), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... complementary DNA was synthesized using the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase point mutant synthesis system (Promega) with random hexamers ...
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bioRxiv - Microbiology 2020Quote: ... a PCR reaction using the PCR Master Mix (Promega) and the primers rplU_Fwd and rplU_Rev (Table 2 ...
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bioRxiv - Genetics 2022Quote: ... PCR was performed using GoTaq PCR Master Mix (Promega) with primers mo37 and ag489 ...
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bioRxiv - Biophysics 2022Quote: ... Two PCR reactions using GoTaq G2 PCR mix (Promega) and a pUC19 template were set up ...
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bioRxiv - Developmental Biology 2022Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using T3 ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA used for the different PCRs were purified by using the Wizard® Genomics Kit (Promega).
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bioRxiv - Bioengineering 2021Quote: ... and digested vector was isolated using the Promega Wizard SV gel extraction and PCR cleanup kit (Promega). Products were then ligated overnight at 16°C following the NEB T4 DNA ligase protocol with 50 ng of pSLQ615 ...
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bioRxiv - Neuroscience 2021Quote: ... was purified using gel clean-up kit (Wizard® SV Gel and PCR Clean-Up, A9281, Promega) (Figure S4B) ...
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bioRxiv - Zoology 2021Quote: ... Those containing single bright bands were purified using Wizard SV Gel and PCR Clean-up Kit (Promega), and sequenced in both directions using PCR primers at Sequetech Inc ...
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bioRxiv - Immunology 2021Quote: Targeted re-sequencing by the Sanger method (Microsynth AG) was performed on kit purified PCR products (Promega) from chromosomal DNA or expression vector templates using pre-mixed sequencing primers listed Table S2.
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bioRxiv - Immunology 2021Quote: ... isotypes from patients were pooled at equal concentrations and concentrated using Wizard PCR Clean-up kits (Promega) according to manufacturer’s instructions with 30 μl of elute ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were ligated into the pGEM®-T vector using a TA cloning kit (A3600, Promega), transformed into E ...
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bioRxiv - Molecular Biology 2022Quote: ... Complementary DNA (cDNA) was synthesized from total RNA using a RT-PCR kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The digested product was gel-extracted using the Wizard SV Gel and PCR Clean-Up Kit (Promega). The two fragments were assembled in a single In-Fusion reaction (In-Fusion HD Cloning Kit ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified using a Nucleospin PCR clean-up kit and subcloned into p-GEM T-easy (Promega). Antisense RNA probes were synthesized using SP6 RNA polymerase and the DIG-labelling kit (Roche ...
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bioRxiv - Biophysics 2022Quote: ... PCR products were separated on 1% agarose gel and purified using a Gel extraction kit (Promega, CA). Sequences of forward and reverse primers are listed in Supplementary Table S21 ...
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bioRxiv - Bioengineering 2022Quote: ... The resulting double-stranded DNA fragments were purified using a PCR Clean-up Kit (Promega, Cat# A9282). The purified DNA was then cloned into the entry vectors in place of the respective SERF using Golden Gate techniques [14 ...
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bioRxiv - Microbiology 2024Quote: ... Gel purified PCR products were ligated into plasmid pGEM-T using the pGEM-T easy kit (Promega) and the resulting plasmid was transformed into Escherichia coli DH5α through electroporation ...
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bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s protocol and reverse transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega) as follows ...
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bioRxiv - Microbiology 2022Quote: In vitro cytotoxicity of SMKIs on mock and/or virus infected A549 cells was determined using CellTiter-Glo 2.0 (CTG) Cell Viability Assay (Promega) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: Cytotoxicity of SMKIs on mock and/or virus-infected PCLS was determined using the LDH-Glo Cytotoxicity Assay (Promega) according to manufacturer’s protocols ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (500ng) was used for cDNA synthesis with mouse Maloney leukemia virus reverse transcriptase (Promega, Madison, WI, USA). SYBR Green RT-qPCR gene expression assays were performed in triplicate in an ABI StepOne Plus thermocycler using appropriate endogenous controls (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... virus was harvested and infectivity assayed by serial titration on HeLa TZM-bl reporter cells using Bright-Glo (Promega).
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bioRxiv - Microbiology 2022Quote: ... First-strand cDNA generation from total RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Relative mRNA quantitation was done using the SYBR green quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The viability of virus- and mock-infected cells were measured using Cell Titer Glow assay (CTG; Promega, Madison, USA). The luminescence was read with a PHERAstar FS plate reader (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and virus entry ability in the selected organs was evaluated by luciferase activity using the luciferase assay system (Promega) and a microplate luminometer ...
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bioRxiv - Microbiology 2021Quote: ... followed by a reverse transcriptase (RT)-step with Moloney Murine Leukemia Virus RT (MMLV-RT, Promega, Madison, WI, USA) in the presence of RNase inhibitor (RNAsin plus ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (MMLV-RT) according to the manufacturer’s protocol (Promega) using 200 ng of total RNA ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized using 1μg total RNA with 200 units moloney murine leukemia virus reverse transcriptase (Promega, Southampton UK), primed with 2μM oligo dT (Thermo Fisher Scientific) ...