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Citations for Promega :
101 - 150 of 4976 citations for Onion Yellow Dwarf Virus OYDV PCR Kits since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Cell Biology 2024Quote: ... and an Avian myeloblastosis virus (AMV) reverse transcriptase from Promega (M5101, Madison, WI, USA). The cDNA and primers were added to Power SYBR Green PCR Master Mix (4367659 ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Neuroscience 2022Quote: ... and the first second PCR kits were obtained from Promega (M7122, Madison, USA).
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bioRxiv - Molecular Biology 2023Quote: ... DNA was then purified using a Promega PCR Clean-Up Kit (Promega #A9282), quantified (Synergy HTX ...
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bioRxiv - Microbiology 2023Quote: ... Real time quantitative PCR was performed using GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Genomics 2024Quote: ... Products were purified with the SV Wizard PCR Purification kit (Promega, WI, USA).
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bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
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bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting complexes were analyzed by primer extension using avian myeloblastosis virus reverse transcriptase (Promega) and a primer (5′-GGTGTGATTGCCTTG ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified PCR products were visualized by blue light illumination and the 504/505 bp bands were excised and the amplified PCR product purified using the Wizard SV Gel and PCR Clean-Up kit (Promega, Madison, WI, USA). Extracted DNA was Sanger sequenced at the University of Michigan Advanced Genomics Core and analyzed for presence of the single base pair deletion using DNAStar LaserGene SeqMan Pro 15 ...
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bioRxiv - Molecular Biology 2023Quote: The DNA was extracted using the Promega PCR Cleaning Kit (Wizard® SV Gel and PCR Clean-Up System, Promega Cat.-No. A9285) as indicated in the manual ...
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bioRxiv - Microbiology 2020Quote: ... PCR products were purified with Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol and directly sequenced using 10F primer to identify individual bacteria isolates at the genus level ...
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bioRxiv - Plant Biology 2021Quote: ... Probes from PCR products were radiolabeled using the Prime-a-Gene kit (#U1100, Promega) in the presence of [α-32P]-dCTP (Hartmann Analytic ...
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bioRxiv - Molecular Biology 2020Quote: ... All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega. All PCR products were examined using the QIAxcel® capillary electrophoresis system 100 (Qiagen).
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bioRxiv - Genomics 2022Quote: ... products were gel purified using the Wizard SV Gel and PCR Cleanup kit (Promega) and ligated into pDONR221-b1b4 (Invitrogen ...
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bioRxiv - Evolutionary Biology 2022Quote: ... amplicons were purified with a WizardTM PCR Clean Up kit (Promega, Fitchburg, WI, USA). PCR products were sequenced using the BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA was purified using the Wizard SV gel and PCR clean-up kit (Promega), denatured at 95 °C for 10 min ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Microbiology 2021Quote: qPCRs were performed with MLV SuMLV primers using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Plant Biology 2021Quote: ... Probes against siRNA populations were PCR products labelled with the Prime-a-Gene kit (Promega). Over-night hybridization at 42°C was in PerfectHyb buffer (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... RNA-isolation for RT-PCR analysis was done using the PureYield™ RNA kit (Promega). For expression analysis of HvTB2 in vrs4 background the vrs4.k mutant (GSHO 1986) ...
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bioRxiv - Microbiology 2022Quote: ... PCR-amplification of both nosZ genes used the Promega GoTaq qPCR kit (Promega, Madison, WI) and 1 μL of DNA template (25-50 ng genomic DNA ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR2 products were cleaned up and concentrated by SV wizard PCR cleanup kit (Promega), Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Bioengineering 2020Quote: ... The first PCR products were purified using a commercial purification kit (Promega, Madison, WI, USA), diluted ...
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bioRxiv - Immunology 2021Quote: ... quantitative real-time PCR was carried out using the GoTaq qPCR Master Mix kit (Promega) with 20 µL of reaction mixture containing gene-specific primers or the PrimePCR assay kit (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The quantitative real-time PCR was performed using the GoTaq qPCR Master Mix kit (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA was purified using the Wizard SV Gel and PCR Clean Up Kit (Promega). Immunoprecipitated DNA was analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... the linearized DNA was purified using the Wizard® SV Gel PCR purification kit (Promega). For the synthesis of 5’-capped mRNA ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2022Quote: ... PCR was run with genomic DNA that was prepared with Wizard Genomic DNA Purification Kits (Promega).
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bioRxiv - Genomics 2023Quote: ... The single-stranded DNA generated using this method was purified using PCR purification kit (A9285; Promega) and quantified using Nanodrop ...
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bioRxiv - Biophysics 2023Quote: ... and extracted each from the gel using a standard protocol from PCR purification kit (Promega, A9282). We then mixed the three fragments together in 10 µl in molar ratio 1:2:1 ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...