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Citations for Promega :
2351 - 2400 of 2934 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Imaging was done in a perfusion chamber with artificial cerebral spinal fluid (ACSF) as described in36 using 1 μM furimazine (Promega), heated to 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were digested using Lys-C (Wako, 1:40, w/w, overnight under gentle shaking at 30°C) and modified trypsin (Promega, 1:60 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated 1:1 for 5 minutes in the dark in opaque 96 well plates with NanoGlo Substrate freshly diluted 1:50 in NanoGlo Buffer (Promega). Luminescence was measured on a GloMax 96 Microplate Luminometer.
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bioRxiv - Molecular Biology 2021Quote: ... cells were co-transfected with 2 µg of pWH464 (pSpCas9(BB)−2A-GFP (pX458)) expressing the corresponding targeting sgRNA and 1 µg of the corresponding donor oligo or plasmid using the Fugene6 (Promega). To generate miR-182Δ and miR-183Δ mESCs ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL RRV-P3 plasmid or 3 μL of cDNA from rose tissue or nuclease-free water (Promega, Madison, WI). The final volume of the LAMP reaction was 25 μL.
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from cells at the exponential phase (OD600 ~1) with the acidic hot phenol method 41 and treated with DNase (Promega). 50 μg of total cellular RNA was incubated with 1 μM of the corresponding DNAzyme ((Supplementary Table S2 ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 µl of the reaction was treated with 1 µl of RNase mixture (10% RNase A, Sigma + 20% RNase One, Promega), incubated at 37°C for 10 min and then mixed with 6 µl of 2 × SDS-PAGE buffer ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with carbon and 0.1% gelatin as described previously (Maeda et al., 2020) and treated with 20 nM HaloTag SaraFluor 650T ligand (GCKA308; Promega) for 15 min before fixation ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Molecular Biology 2021Quote: ... Liberibacter OmpA was amplified using SgfI and PmeI restriction sites (Table 1) and was digested using Flexi Enzyme Blend (Promega). Similarly ...
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bioRxiv - Molecular Biology 2021Quote: ... The GAL1 promoter was amplified from yeast genomic DNA using primers 4588-1/XH5103 and ligated into pGEM-Teasy (Promega) to create pCB4554 ...
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bioRxiv - Systems Biology 2020Quote: ... to a final concentration of 1% DOC before overnight digestion at 37 °C with lysyl endopeptidase (Wako Chemicals) and sequencing-grade porcine trypsin (Promega) at an enzyme-substrate ratio of 1:100 for both ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was removed and pellet was resuspended in 400 μl of sort buffer (1 mM EDTA 0.2 U/μl RNase inhibitor (Promega, N211B), 2% BSA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 20 pmols of folded pre-tRNA substrate was incubated with a final concentration of 5 U ml−1 RNasin plus inhibitor (Promega), 5 mM ATP and 8 pmols of TSEN complex in a final reaction volume of 20 μl for 1 h at 30 °C ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized via reverse-transcription of 1 μg of RNA using M-MLV Reverse Transcriptase (Promega (Wisconsin, USA). cDNA was used to analyse differences in gene expression by qRT-PCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2021Quote: ... JEG-3 viability after 1 h salubrinal pretreatment followed by 48 h ZIKV infection was measured by CellTiter-Glo Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then washed three times with 1 × PBS and blocked with 3% Blot-Qualified bovine serum albumin (BSA, Promega) in 1 × PBS containing 0.05% Tween-20 (TPBS ...
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bioRxiv - Biochemistry 2021Quote: ... Twenty microliters of HPLC-grade water were added to reduce urea concentration and trypsin digestion was performed using a 1:20 (enzyme:protein) ratio of sequencing grade modified trypsin (Promega, V5111) and 18 h incubation at 37 °C on a ThermoMixer (Eppendorf ...
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bioRxiv - Biochemistry 2020Quote: ... CDK2-cyclin A-SKP1-SKP2 (full-length SKP1 and full-length wild-type SKP2 and S64A mutant) and CDK2-cyclin A-p27KIP1M (residues 1-106) were assayed using the ADP-GloTM assay (Promega) using a p107 peptide (peptide SPIK+RXL ...
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bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were diluted 1:5 with 100 mM ammonium bicarbonate and digested overnight at 37°C following the addition of 1 µg of Trypsin/Lys-C protease mixture (Promega) and calcium chloride to a concentration of 1 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... Collagenase IV (all at a concentration of 1 mg/ml) and Dispase (2mg/ml) in the presence of RNase inhibitors (RNasin (Promega) and RNase OUT (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with the ImProm-II Reverse Transcription System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... ShhN dsDNA coding for residues 1-198 of murine Shh was obtained by PCR using Pfu polymerase (Promega cat # M7741) and the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... Firefly and renilla luciferase activities were measured using an Optocomp 1 luminometer and a Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
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bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg of DNase treated RNA was used for cDNA synthesis by oligo-dT primers and MLV reverse transcriptase (Promega) enzyme ...
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bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
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bioRxiv - Microbiology 2021Quote: ... A series of concentrations from 50-600nM in symmetric and asymmetric forward and reverse primer concentrations were added to the RT-qPCR reaction using the GoTaq 1-Step RT-qPCR System (Promega) according to the manufacturer’s instructions using the standard annealing temperature of 60°C ...
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bioRxiv - Genomics 2021Quote: ... Inserts were cut out with appropriate restriction enzymes (Suppl. Table 1) and ligated with pre-digested pGL4.23[luc2/minP] (Promega, # E8411) plasmid upstream of a minimal promoter and a firefly luciferase gene ...
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bioRxiv - Microbiology 2021Quote: ... A 10µl of sample was mixed with a 50µl portion of GAR-1 reagent (targeting Systems) and then measured in the luminometer (Promega, USA)36.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using a 1:3 ratio of human μOR and a splitluciferase based cAMP biosensor (pGloSensorTM-22F; Promega). Transit 2020 (Mirus Biosciences ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... To the first pool 1 µl/mL DMEM/F12 (no phenol red) of HaloTag® NanoBRET™ 618 Ligand (Promega) was added and to the second pool DMSO ...
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bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug (for cellular RNA) and 20 ng (for EV RNA) was treated with 1 U of DNAse I RNAse-free (#EN0521 PROMEGA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the pSARP-12R4-9 input library with a 3:1 transfection reagent:DNA ratio using Fugene 6 transfection reagent (Promega #E269A). At 72 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...