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Citations for Promega :
2251 - 2300 of 2934 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Urea concentration across control and sample tubes were dropped to ∼2M with 20 mM HEPES pH 8.0 and were digested with 1 μg sequencing grade trypsin (Promega) overnight at 37°C in a nutator ...
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bioRxiv - Microbiology 2023Quote: ... 300 µl of eluant (at 1.5 mg/ml) was treated with 6.5 µl of 1 mM HaloTag Alexa Fluor 488 Ligand (Promega PAG1001). Both labeled and unlabeled proteins were subject to size exclusion chromatography for further purification using Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: pcDNA5/FRT/TO-SFB-HELB (WT, D506G, and 3xA) plasmids (1 µg) were transfected into 1.0 × 105 cells using FuGENE HD (Promega) according to the manufacturer’s protocol followed by incubation at 37°C with 5% CO2 24 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then transfected with plasmid DNA (4µg per plasmid per flask) using FuGENE HD (1:3 ratio; Promega) and cultured at 28°C.
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bioRxiv - Microbiology 2024Quote: ... At 1 day after the treatment the reporter assay was performed using Dual-Glo Luciferase System (Promega, Cat# E2920) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified after migration on a 1% agarose TAE 0.5X gel using the Wizard PCR Clean-Up system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQTM SYBR® Green qPCR Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... proteolytic digestion was performed by adding Trypsin (0.1 μg/μL in 1 mM HCl, Sequencing Grade Modified Trypsin, Promega) at a ratio of 50:1 (w:w ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μL 1× Luciferase Cell culture lysis buffer (E1531, Promega) for 15 min at room temperature and centrifuged for 10 min at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Bioengineering 2023Quote: ... PB-CAG-H2B-mCherry-IPuroR and pCMV-hyPBase were co-transfected into KhES-1 using Fugene 6 (Promega, E269A) transfection reagent following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labelled antisense riboprobes were synthesized from cloned cDNA fragments (Supplementary file 1) using T7 or SP6 polymerases (Promega) and digoxigenin-labelled dUTPs (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng pCas9 mCherry Frame +1 and 100ng pCRISPaint HaloTag-Puro donor plasmids were transfected using ViaFect (Promega E498A). Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed using a trypsin solution with a concentration of 1 μg /μL (Trypsin Gold, V528A, Promega), and 0.6 μL of the trypsin solution was added to achieve a final ratio of 1:50 ...
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bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: Cells stably expressing epitope-tagged NK1R were transiently transfected (Lipofectamine™ 3000 Transfection Reagent) with 1 μg of pGL4.29[luc2P/CRE/Hygro] or pGL4.30[luc2P/NFAT-RE/Hygro] plasmids (Promega Corp.), seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck], 1 unit/mL RNasin Ribonuclease Inhibitor [N2515, Promega]) and frozen at -80 °C.
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bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... protoplasts were transferred onto BCD-growth medium containing ammonium tartrate and geneticin G418 antibiotic (50 µg ml−1; Promega) followed by two weeks growth period without the antibiotic and second selection period for two weeks with antibiotic again.
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Microbiology 2022Quote: ... twice and 20 µL of a 1:19 mixture of substrate:buffer from the Nano-Glo Live Cell Assay (Promega) were added to 80 µL of OptiMEM per well ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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bioRxiv - Neuroscience 2022Quote: ... 1–5 µl of gDNA was mixed with 0.5 µl of 10 mM dNTP mix (Promega, C1141, Madison, WI), 10 µl of 25 mM MgCl2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Precipitates were solubilised in 8M urea (20mM HEPES, pH 8) and digested with Lysine C (1:50 ratio; Promega) for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio ...
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bioRxiv - Bioengineering 2022Quote: ... samples were diluted 1:10 in 50 mM ammonium bicarbonate before the addition of 1:50 protein:protein by mass of sequencing-grade trypsin (Promega) for overnight digestion at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... DTT was added to a final concentration of 3 mM before adding 1 μg of sequencing-grade trypsin (Promega) and incubating at 37 °C overnight ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Genetics 2022Quote: ... Samples were then diluted 10fold with 20 mM Tris and trypsin (Trypsin Gold, Promega, #V5280, 1:200 w/w) and digested overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Molecular Biology 2023Quote: ... ncBAF (Brd9) and PBAF (Baf180) (supplemental table 1) were isolated by using the Pure yield plasmid midiprep system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from leaves or bacterial liquid cultures via phenol/chloroform extraction and treated with DNase 1 (Promega) to remove gDNA contamination ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.