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Citations for Promega :
2201 - 2250 of 2934 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... plates were washed 5 times with wash buffer (PBS with 1% v/v bovine serum albumin (Capricorn Scientific) and 0.05% v/v Tween-20 (Promega)) ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Immunology 2021Quote: ... SIV pseudovirus Env construct was cotransfected with env-deficient backbone plasmid (pSG3ΔEnv) in a 1:2 ratio with transfection reagent FuGENE 6 (Promega) in HEK 293T cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:1000 dilution and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary IgG (W401B, Promega) was used in a 1:20,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with 0.1M ammonium bicarbonate solution to a final concentration of 1% sodium deoxycholate before digestion with trypsin (Promega) at 37°C overnight (protein to trypsin ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 minutes in the dark, RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, overnight at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Microbiology 2020Quote: ... the medium was removed and the cells were lysed with 30 µl/well of 1× cell lysis buffer (Promega) for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Immunology 2020Quote: ... The HIV-1 infectivity was evaluated by the luminescence intensity following Steady-Glo® luciferase assay (Promega; WI, USA), following company specified methodology ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmIBH1-1 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat#G184A) (Promega). ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Immunology 2020Quote: ... Samples were then diluted with 50 mM HEPBS pH 8.3 to reduce urea concentration down to 1 M and digested with trypsin (Promega) at 1:10 enzyme:substrate ratio overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... MI) and digested overnight in 25 mM ammonium bicarbonate (ambic) with 1:200 (w/w) dilution of trypsin (Promega) for mass spectrometric analysis ...
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bioRxiv - Cell Biology 2020Quote: ... then diluted to a final concentration of 1 M prior to in-solution digestion with 0.2 µg/µl trypsin (Sequencing grade (Promega) overnight at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed by adding 3x lysis buffer (10 mM Tris-HCl, 1.5% Igepal, 150 mM NaCl, 1 U/µl RNasin (Promega)) ...
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bioRxiv - Biochemistry 2020Quote: ... for 4 h at 37°C and then the urea concentration was reduced to 1.5 M and digested was finalized with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (M1705, Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Microbiology 2022Quote: ... twice and 20 µL of a 1:19 mixture of substrate:buffer from the Nano-Glo Live Cell Assay (Promega) were added to 80 µL of OptiMEM per well ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
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bioRxiv - Neuroscience 2022Quote: ... 1–5 µl of gDNA was mixed with 0.5 µl of 10 mM dNTP mix (Promega, C1141, Madison, WI), 10 µl of 25 mM MgCl2 ...
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bioRxiv - Cancer Biology 2022Quote: ... Precipitates were solubilised in 8M urea (20mM HEPES, pH 8) and digested with Lysine C (1:50 ratio; Promega) for 4h at 37C in 6M urea followed by an overnight trypsin digestion (1:25 ratio ...
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bioRxiv - Bioengineering 2022Quote: ... samples were diluted 1:10 in 50 mM ammonium bicarbonate before the addition of 1:50 protein:protein by mass of sequencing-grade trypsin (Promega) for overnight digestion at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... DTT was added to a final concentration of 3 mM before adding 1 μg of sequencing-grade trypsin (Promega) and incubating at 37 °C overnight ...
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bioRxiv - Genomics 2022Quote: ... Single nuclei were sorted into 384 well plates containing 1 µl M-Digestion Buffer containing proteinase K and ~0.05 pg Lambda DNA isolated from dcm+ E.Coli (Promega #D1501).
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bioRxiv - Genetics 2022Quote: ... Samples were then diluted 10fold with 20 mM Tris and trypsin (Trypsin Gold, Promega, #V5280, 1:200 w/w) and digested overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: Edited loci were amplified using locus-specific primer pairs described in Supplementary Table 1 using GoTaq master mix (Promega) and 200ng of genomic DNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the cells were preincubated with 50 µl of a mix of Nluc substrate endurazine (1:50 dilution; #N2571, Promega) and LgBiT (1:50 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Molecular Biology 2023Quote: ... ncBAF (Brd9) and PBAF (Baf180) (supplemental table 1) were isolated by using the Pure yield plasmid midiprep system (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA reverse transcription and cDNA amplification was then carried out by the GoTaq 1-Step RT-qPCR System (Promega), using established primers (31) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL per input or ChIP sample was used in a qPCR using the GoTaq® Mastermix (#M7133, Promega) and primer pairs for the amplification of the Ifnb1 promoter region (ChIP_IFNb1_fwd 5’-GCCAGGAGCTTGAATAAAATG ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from leaves or bacterial liquid cultures via phenol/chloroform extraction and treated with DNase 1 (Promega) to remove gDNA contamination ...