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Citations for Promega :
2601 - 2650 of 2934 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... An amount corresponding to 5 % of the pull-down volume was saved for Western blot analyses and 1 µg of trypsin gold (Promega) was then added to the beads for 16 h at room temperature in a thermomixer at 600 RPM ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of cell lysates were diluted 1:30 and assessed for firefly and Renilla luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... elution buffer I (2 M Urea, 50 mM Tris pH 7.5, 1 mM DTT and 5 ng/µl Trypsin (Promega, #V5111)) were added and samples were incubated for 1 h at RT ...
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bioRxiv - Immunology 2024Quote: ... Blots were incubated with primary antibodies overnight at 4°C followed by 2-hour incubation with HRP-conjugated secondary anti-rabbit IgG-HRP conjugate antibodies (1:10,000; Promega W401B) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Secondary antibodies were diluted in blocking buffer and incubated with the membrane for 60 min at room temperature (1:10,000 anti-rabbit-HRP, Promega #W401B). After secondary antibody incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by a digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... For cDNA synthesis up to 1 µg of total RNA was reverse transcribed using random hexamer primers and M-MLV Reverse Transcriptase (Promega). Gene expression was measured using the primaQUANT CYBR qPCR mastermix (Steinbrenner Laborsysteme GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... The complementary DNA (cDNA) synthesis was performed with 1 μg of total RNA using ImProm-II™ Reverse Transcriptase (Promega). cDNA was diluted in DNase-free water and used for qRT-PCR with Go Taq® qPCR Master Mix reagent (PROMEGA ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was clarified and combined with an equal volume of a 1:50 Nano-Glo Luciferase Assay Substrate: Nano-Glo Luciferase Assay BuKer (Promega). Luminescence was read at 200 gain on the BioTek Cytation5 (Agilent Technologies).
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bioRxiv - Microbiology 2024Quote: ... After three rounds of washing in PBST the following Horseradish peroxidase-conjugated secondary antibodies were diluted in 5% milk-PBST and used with at a 1:10000 dilution anti-rabbit (W4018, Promega), 1:5000 anti-mouse (W402B ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then diluted with 50mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... The reactions were subjected to primer extension at 42[°C for 45[min using 1 unit of MMLV-RT (Promega) and 0.5[mM of dNTPs ...
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bioRxiv - Physiology 2024Quote: ... membranes were incubated at room temperature in anti-rabbit or anti-mouse IgG (Promega, Cat# W401B and W402B, 1:10,000) for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from half a volume of total RNA (1 μg) treated for 30 min at 37°C with RQ1 RNase-free DNase (Promega) using a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed on 1 μg total RNA using an oligo (dT)18 primer and MMLV reverse transcriptase (Promega). For qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... We harvest 1 OD600 unit of yeast in mid-exponential growth phase and resuspended it in 100 μl Passive Lysis Buffer (Promega) with mixing for 2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... the precipitated proteins were dissolved by 100 mM NH4HCO3 and digested overnight by trypsin (trypsin: protein ratio,1: 50, Promega) at 37°C ...
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bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
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bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... The transfected cells were treated with 5 ng/ml TGFβ1 or vehicle for 15 min and Luciferase activities were measured using the Dual-Luciferase Reporter Assay Kit (E1910; Promega).
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bioRxiv - Synthetic Biology 2024Quote: ... encoding each aaRS was incubated at 30 °C for 8 h in a customized PURE system (version 1) containing fluorescently labeled lysyl-tRNA (FluoroTect GreenLys, Promega). After expression ...
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bioRxiv - Genomics 2024Quote: ... Reverse transfection was done using a ratio 3:1 volume-to-mass ratio of FuGENE6 (0.3 µL reagent: 100 ng of DNA per well, Promega E2691). The transfection mix included the following vectors ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL of an aliquot of the reaction mixture was added to 30 µL of the luciferase assay reagent (Promega), and luminescence was measured as translational activity using a luminometer (GloMax ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL of an aliquot of the reaction mixture was added to 30 µL of the Luciferase Assay Reagent (Promega), and luminescence was measured as translational activity using a luminometer (GloMax ...
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bioRxiv - Biochemistry 2024Quote: ... and wild-type or mutant βarr1 with a C-terminal HA tag with a 1:5 DNA:FuGENE®6 (Promega) ratio according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 99 ng of RRE reporter plasmid (pCMV gagpol-IRES FFL-RRE) and 1 ng of the reference plasmid pNL1.1.TK [Nluc/TK] (Promega, N1501) were co-transfected with Lipo-fectamine™ 3000 (Invitrogen™ ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then digested with 1 μg of Lysyl-endopeptidase (Wako) at room temperature for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of the reaction product was combined with 30 μL of a 1:50 dilution of Nano-Glo substrate (Promega) into a 364-well microplate ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein : enzyme by weight, Promega V5280) at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested at room temperature overnight using 1:100 (m/m) sequencing grade trypsin (cat. no. V5111, Promega, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Thermolysin proteolysis was performed in 100uL of 50mM ABC/10mM KCl (pH=8) by adding Thermolysin (Promega, 1:50 ratio) to proteins and incubating at 70°C for 1 hour at 900 rpm prior to acidification to 1% FA ...
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bioRxiv - Cell Biology 2024Quote: ... A 250 ug was alkylated and digested by incubating overnight at 37oC in trypsin at a ratio of 1:50 (Promega). Digestion was acidified by adding formic acid (FA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips to ensure surface cleanliness and cell adherence at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1 μg of plasmid DNA using 3 μL Fugene HD transfection reagent (Promega). HaloTagged AP2-σ2 was visualized by adding the JF646-HaloTag ligand (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were washed with 100 µL ABC and supernatant was transferred to the digestion and the mixture was digested with 1 µg Trypsin (Promega) overnight at RT ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were dissolved in 40 mM ammonium bicarbonate and digested by 1 ug sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
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bioRxiv - Cell Biology 2020Quote: ... Heterologous expression of proteins was achieved by transfecting cells with 1 □g of plasmid DNA/35 mm using FuGENE HD (Promega; E2311), as previously described (Webb et al. ...
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bioRxiv - Cell Biology 2020Quote: ... 250 ng of each of the donor and Cas9-sgRNA vectors were transfected to RPE-1 cells cultured in 6 wells with 1.5 μl of FuGENE® HD (Promega, E2311) overnight ...
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bioRxiv - Genetics 2021Quote: ... cell supernatant from untransfected and cells transfected with IL2 constructs was diluted 1:12 with complete media and used to perform the IL2 Bioassay (Promega, JA2201). Luminescence (RLU ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... and then resuspended in tagmentation reaction mix for 30 min (2.5uL TD1, 1X TD Buffer, Illumina Nextera DNA, FC-121-1030; .25uL 1% digitonin, Promega G9441; 1xPBS;) gently shaking (300rpm on Eppendorf thermomixer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113).