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Citations for Promega :
2301 - 2350 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The 400 base pair region surrounding the sgRNA target was then amplified by PCR (forward primer: 5’-CCCAGAGAGGAGGCTGTAGA-3’; reverse primer: 5’-AAAGGCCTCCCAGGGGTTAT-3’) with GoTaq DNA Polymerase (Promega). The resulting PCR product was cloned into the pCR 4-TOPO vector using the TOPO TA Cloning Kit for Sequencing (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Developmental Biology 2023Quote: The highly conserved 998-bp core sequence of Xenopus tropicalis MFCS1 was amplified by PCR with specific primers (Table S3) and inserted into the Acc65I and XhoI sites of the pGL4.23 vector (Promega, E8411). Then the minimal promoter of the pGL4.23 vector was replaced with a 486-bp sequence of Xenopus laevis shh promoter (Fig ...
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bioRxiv - Bioengineering 2023Quote: ... Presumptive screening of 10 colonies per mPilA variant was performed via colony-PCR using GoTaq® Green Master Mix (Promega) and forward and reverse primers Diag:71301:MPilA:All:FWD and Diag:71301:MPilA:All:REV (SI Table S1) ...
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bioRxiv - Neuroscience 2023Quote: ... of neuropeptide and neurotransmitter marker genes were PCR-amplified from zebrafish brain cDNA or genomic DNA and cloned into the pGEM-T Easy vector (Promega). Three glutamate decarboxylase genes (gad1a ...
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bioRxiv - Plant Biology 2023Quote: ... Genotyping PCRs were performed using Promega Hotstart Green MasterMix following the recommended conditions from the manufacturer (Promega, Madison, WI, U.S.A.).
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Sequences encompassing the SD site and the env gene were acquired after cloning the PCR fragments inserted into pGEM-Teasy (Promega). Sanger sequencing with primers env7254(+ ...
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bioRxiv - Microbiology 2023Quote: ... gondii glyceraldehyde 3-phosphate dehydrogenase 2 (GAPDH2, ML5049/ML5680) or TgAPT1 (ML4097/ML4098) were used for subsequent PCR amplification with GoTaq polymerase (Promega) for twenty-five cycles as follows ...
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bioRxiv - Plant Biology 2023Quote: The cDNA of BdNRT2A was first amplified by PCR using specific forward primer 70F2 and reverse primer 70R2 before being cloned into the pGEMT-easy vector (Promega). Clones with correct sequence were used to produced appropriate PCR product for Gateway cloning ...
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bioRxiv - Microbiology 2023Quote: The region upstream of the genes of interest was amplified by PCR with proper primers (Table S4) and cloned into the pGEM-T easy plasmid (Promega). The insert was removed by digestion and subcloned into the pRKlacZ290 vector to generate transcriptional fusions to the lacZ gene ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR fragment was directionally cloned downstream of the Renilla luciferase ORF in the psiCHECK-2 vector (Promega, Madison, US) using the Quick Ligase kit (M2200 ...
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bioRxiv - Cell Biology 2023Quote: The Human SART1 mutant resistant to siSART1#3 and its deletions mutants (amplified by PCR) were subcloned into pCI-neo Mammalian Expression Vector (Promega).
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bioRxiv - Systems Biology 2023Quote: ... and the bands at the predicted product size were excised and purified with a gel and PCR clean-up system (Promega). Sanger sequencing was completed by the Micromon facility at Monash University ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleofected cells were incubated for 48 h prior to clonal amplification and screening for homozygous clones using target-specific PCR (GoTaq Hot Start, Promega) on genomic DNA using the following primers ...
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bioRxiv - Cell Biology 2024Quote: ... the Deaf1 promoter region was first PCR-amplified from genomic DNAs of C2C12 mouse myoblasts and cloned into the pGL4.20 vector (Promega, E675A). ...
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bioRxiv - Microbiology 2024Quote: The copy number of re-integrant strains was determined by extracting the genomic DNA and performing quantitative PCR (qPCR) using GoTaq polymerase (Promega) and a StepOnePlus real-time PCR machine (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... named rpsL*) was amplified by PCR with primers BlD-LLcfusARpsL/BlD-LLldacARpsL and cloned into the pGEM-T Easy vector (Promega), yielding plasmid pGEM-rpsL* ...
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bioRxiv - Immunology 2024Quote: ... miiuy MDA5 gene was amplified using PCR with the gene-specific primers and ligated into a pmir-GLO luciferase reporter vector (Promega). Meanwhile ...
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bioRxiv - Cell Biology 2023Quote: ... Nucleofected cells were incubated for 48 h prior to clonal amplification and screening for homozygous clones using target-specific PCR (GoTaq Hot Start, Promega) on genomic DNA using the following primers ...
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bioRxiv - Synthetic Biology 2023Quote: ... The extracted plasmid was linearized by SalI digestion and purified using the Wizard SV PCR cleanup system (Promega, Madison, WI). SMRT sequencing was performed using Sequel IIe (Pacific Biosciences ...
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bioRxiv - Plant Biology 2024Quote: For in vitro pulldown experiments the coding region of AtMED25Δq was fused N-terminally with a flag-tag by PCR with oligonucleotides containing restriction site overhangs for cloning into pF3A WG BYDV (Promega). RAP2.12 C-terminally fused to CFP was previously established (Schmidt et al. ...
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bioRxiv - Zoology 2024Quote: ... The amplicons-containing were excised after gel-electrophoresis and extracted DNA by using Wizard SV Gel and PCR Clean-Up System (Promega). The amplicon DNAs were further analyzed for Sanger sequencing with the QuantumDye Terminator Cycle Sequencing Kit (Tomy Digital Biology ...
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bioRxiv - Plant Biology 2023Quote: ... Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega). Sequencing was performed at LGC Genomics (Berlin ...
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bioRxiv - Microbiology 2024Quote: ... Kanamycin-sensitive exconjugants were screened for the mutant with colony PCR using altgenoF and altgenoR primers designed outside of the deletion flanks with the following PCR reaction mix: 10 µl of GoTaq Green master mix (Promega), 0.5 µl of each primer at 10 µM concentration ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL of extracted DNA was added to an amplification mixture containing 1 µl (10 µM) of primers and 12.5 μl of PCR Master Mix Plus (Promega, USA). To each mixture ...
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bioRxiv - Cell Biology 2024Quote: Genotyping was performed by PCR in 20 µl volume reactions that included 1x GoTaq Green Mas-ter Mix (Promega #M7123), 1.1 mM MgCl2 ...
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bioRxiv - Molecular Biology 2024Quote: ... The resultant PCR products were subsequently subjected to purification through agarose gel electrophoresis employing the Wizard Sv Gel Clean-up system (Promega). Following purification ...
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bioRxiv - Microbiology 2024Quote: ... and the resulting products were checked on agarose gel (1%) and purified using the Wizard SV Gel and PCR Clean-Up System (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... The master mix for the PCR reaction included 10 µL GoTaq Hot Start Green Master Mix (Promega, Madison, WI US), 2 µL of each primer at 10 µM ...
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bioRxiv - Developmental Biology 2024Quote: ... Amplicons were separated by electrophoresis and the corresponding bands were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) kit ...
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bioRxiv - Cell Biology 2024Quote: ... Deletion mutants of Caskin2 and Caskin2-4A were generated by site-directed mutagenesis with the PCR-based overlap extension method using Pfu DNA polymerase (Promega), and fragments containing the different mutations were exchanged with corresponding fragments in the pUC19-CASKIN2-GFP plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... The following primers were used to amplify PCR products which were used to generate in situ hybridization probes with T7 RNA polymerase (Promega) and Digoxigenin-UTP (DIG RNA Labeling Mix ...
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bioRxiv - Microbiology 2023Quote: ... using oligo (dT) as a primer and quantitative PCR analysis was performed using the GoTaq qPCR SYBR master mix (Promega) on a LightCycler 480 instrument (Roche) ...
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bioRxiv - Biophysics 2024Quote: ... was incorporated at the 5’ end of the FL open reading frame during PCR amplification from a pRL-CMV vector (Promega). A Kozak consensus sequence and a 50-nucleotide upstream region was incorporated before the translation start site to ensure enough space for the assembly of translation initiation complex77 ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA fragments were PCR-amplified with primer pairs containing a XhoI and BamHI site from human genomic DNA (Promega) and subcloned into the pCLL-NoPromoter-FLuc-CMV-RLuc-dsRed2 vector ...
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bioRxiv - Immunology 2023Quote: ... Double stranded RNA (dsRNA) was synthesized from purified T7-tagged PCR amplicons using the T7 RiboMax Express Large-Scale RNA production system (Promega) according to the manufacturer’s instructions and purified as previously described [33] ...
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bioRxiv - Plant Biology 2020Quote: ... carried out using the RQ1-DNase kit (Promega), and were reverse transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... using the Wizard Genomic DNA Purification Kit (Promega). Cytosine methylation on target loci was assessed by Methylation-Sensitive Restriction Enzyme qPCR (MSRE-qPCR ...
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bioRxiv - Microbiology 2020Quote: Wizard Promega DNA extraction kits (Promega, Madison, WI) was used to extract DNA from overnight broth culture ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... using the GoTaq qPCR Master Mix kit (Promega). Gene expression was normalized to the housekeeping gene Rplp0 and expressed as fold change compared to CD11cWT samples ...
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bioRxiv - Genomics 2020Quote: ... with the Wizard Genomic DNA Purification Kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The RealTime-Glo Annexin V Apoptosis kit (Promega) was used as instructed to quantify the degree of apoptosis in HCASMC ...
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bioRxiv - Neuroscience 2021Quote: ... purified with Wizard Genomic DNA Purification kit (Promega) and confirmed via sequencing (GeneWiz) ...
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bioRxiv - Evolutionary Biology 2019Quote: The Wizard® Genomic DNA Purification kit (Promega) was used to isolated genomic DNA from overnight cultures ...
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bioRxiv - Microbiology 2019Quote: ... using the LEV DNA kit (AS1290, Promega, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Dual-Glo® Luciferase Assay System Kit (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by cDNA synthesis (Promega and Fermentas kits) and SYBR Green (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... were determined via Quantifluor dsDNA assay kit (Promega), modified 1,9-dimethylmethylene blue (DMMB ...