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Citations for Promega :
2101 - 2150 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and NADP/NADPH-Glo Assay kit (Promega), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... using Fugene 6 Transfection Reagent kit (Promega) according to manufacturers’ instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Molecular Biology 2021Quote: ... and the final PCR product was gel-purified and subcloned into the pGEM-T Easy Vector (Promega, Guangzhou, China) for sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... a region of the COX1 gene was amplified by PCR using GoTaq® G2 Green Master Mix (Promega, Australia) and primer set LepF (5’-TTCAACCAATCATAAAGATATTGG-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... and SREBP-2 (NM_004599) expression plasmids were prepared by subcloning PCR products into a mammalian expression plasmid pCI (Promega). Likewise ...
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bioRxiv - Developmental Biology 2021Quote: ... Sequences of interest were amplified from the cDNA through PCR and ligated into the pGEM-T-Easy vector (Promega). Ligation products were cloned into JM109 cells ...
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bioRxiv - Immunology 2022Quote: ... on memory CD8 T cells were quantified by qRT-PCR using a final 0.5 μM concentration of each primer and SYBR Green GoTaq qPCR Master Mix (Promega). Amplification was performed in a StepOne Plus Real time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... The resulting amplicons were pooled by sample and purified using the Wizard SV-Gel and PCR Cleanup System (Promega). Samples were sequenced by the UW-Madison Biotechnology Center on an Illumina Hi-Seq 2500 in rapid mode ...
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bioRxiv - Synthetic Biology 2019Quote: Two to three colonies of TOP10 transformed cells were selected to verify through PCR with GoTaq DNA Polymerase (Promega) if the plasmids they contained were the desired constructions ...
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bioRxiv - Biochemistry 2019Quote: ... After confirmation of their amplicon size by 0.8% Tris-Acetate EDTA agarose gel electrophoresis and purification by Wizard SV Gel and PCR Clean-Up System (Promega), the sequences of their ORFs were confirmed using BigDye Terminator V3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... 2-3 μl of the clear part of the solution was used for PCR with either Gotaq (Promega, M7808) or LongAmp 2X (NEB ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
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bioRxiv - Microbiology 2020Quote: Approximately 105 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert:vector and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... and purified by gel extraction using the Wizard SV gel and PCR clean-up system (Promega, Fitchburg, WI, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was inserted into the multiple cloning site (MCS) of the psicheck-2 vector (supplied by Promega) downstream of the Renilla luciferase gene according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Presence or absence of transcript was detected by a regular PCR using GoTaq® G2 DNA Polymerase (Promega, M7841) from 100ng cDNA and visualized on an agarose gel.
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bioRxiv - Microbiology 2022Quote: ... The multiplex PCR was performed in a 12.5 μl volume containing 2.5 μL of 5x GoTaq® Reaction Buffer (Promega), 0.25 μL of dNTP (10 mM) ...
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bioRxiv - Microbiology 2022Quote: Each colony PCR consisted of a 25-μl reaction containing: 12.5 μl 2x GoTaq Green Master mix (Promega, UK), 0.5 μl of the reverse and forward primers (10 μM each) ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega). All mixtures were made in 1x NEBuffer 3.1 on ice with final concentrations of 80nM Cas9 ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng of starting RNA equivalent cDNA was subjected to PCR reaction with the GoTaq green master mix (Promega) and 30 amplification cycles (94°C for 30 s ...
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bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
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bioRxiv - Microbiology 2020Quote: Illumina P5 and P7-barcoded adaptors were added by PCR on gDNA samples according to the GoTaq protocol (Promega). PCR amplicons were gel purified and sequenced on a HiSeq4000 (Illumina ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into a T Easy plasmid using the pGEM®-T Easy Vector (Promega, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The 1400 bp long DNA fragments were purified (Wizard®SV Gel and PCR Clean-Up System, Promega, Germany) and sequenced using the same 27f and 1492r primer pair ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was subjected to adenine overhanging and inserted into pGEM T-easy vector (Promega, Madison, WI, USA) for sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... pKS was nicked with Nt.BbvCI and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Cat#A9281). The resulting short ssDNA fragment was then replaced with a 5-fluoro-2’-deoxycytidine (FdC)-modified oligonucleotide (400 nM ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2020Quote: ... The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Molecular Biology 2019Quote: ... The two fragments were then ligated using an overlap extension PCR reaction and ligated into pGEM/T-Easy (Promega). Positive clones were digested with AscI and SalI and the dropout was subsequently ligated into an AscI- and SalI-linearized pENTR-PpU6-sgRNA-NGG plasmid.
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...
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bioRxiv - Molecular Biology 2020Quote: Outward facing PCR reactions were performed on 6ng of random primed cDNA using the GoTaq G2 DNA polymerase (Promega) following the manufacturer’s recommendations in the Techne TC-3000 PCR thermal cycler (initial denaturation of 2 min 95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... STR profiling was carried out by PCR amplification of nine STR loci plus amelogenin (GenePrint 10 System; Promega, #B9510), fragment analysis (3730XL DNA Analyzer ...
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bioRxiv - Pathology 2021Quote: ... The DNA concentration of each PCR product was determined using a QuantiFluor™-ST fluorescent quantitative system (Promega, USA), and mixed with the appropriate proportion based on sequencing requirements ...
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bioRxiv - Microbiology 2020Quote: The PCR conditions for 50 μL were 25 μl of GoTaq G2 Hot Start Green Master Mix (#M7423, Promega), 0.5 μM of each primer (Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega, Madison, WI), resulting in the construction of pCjNC110::cat which was then transformed into chemically competent E ...
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bioRxiv - Microbiology 2021Quote: ... Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The library was amplified for six PCR cycles with short primers followed by a ProNex size selection step (Promega) in a 1:2.95 (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Regions of interest were amplified in a PCR reaction with GoTaq G2 Flexi DNA polymerase (Promega, Madison, WI, USA), and PCR-amplified samples were run on a 1,2% TBE-agarose gel for size separation ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The presence and orientation of DNA fragments were confirmed by colony PCR using GoTaq Green Master Mix (Promega, USA), whereas the sequence of the genetic construct was checked by Sanger sequencing reactions (Macrogen Inc. ...
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bioRxiv - Neuroscience 2022Quote: Riboprobes for in situ hybridisation were generated by cloning transcript-specific PCR products into pGEM-T-Easy (Promega, A1360) using the TA-cloning method ...
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bioRxiv - Genetics 2022Quote: ... The corresponding bands were cut out and purified using wizard SV gel and PCR clean-up system (Promega, A9282). The concentrations of purified PCR products were measured by Nanodrop 2000c and then diluted into 70-100ng/uL with distilled water accordingly ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments with expected size were purified and TA cloned in the pGEM-T Easy Vector (Promega, Madison, WI) for DNA sequencing ...